Faculty of Veterinary Medicine

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    THE INFLUENCE OF THE LEPTIN GENE ON MILK PRODUCTION IN THREE CONSECUTIVE LACTATIONS IN HOLSTEIN FRIESIAN CATTLE
    (Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02)
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    Markov, Nikolay
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    This study uses a PCR method to detect allelic polymorphisms of the LEP gene. The study was conducted among 100 Holstein cows owned by private breeders. The study was conducted on Pelagonija farm, located in the Southwestern part of Republic of North Macedonia, over a period of three lactations or 915 days. The research focused on the health status of the animal and animal welfare. This study will identify all genotypes of the LEP gene and determine associations between the polymorphism of the leptin gene and the dynamics of milk production during three lactations of the cows. The best indicators for all three lactations will be identified in the group of animals with the TT genotype of the LEP gene. These individuals are characterized by increased milk yield, a high yield index and a tendency to increase the average daily milk yield during three lactations. In cattle, the LEP gene provides instructions for synthesizing leptin, a protein hormone predominantly expressed by white adipocytes (fat cells). Leptin's physiological roles include the regulation of energy intake, body mass, immune competence, and reproductive function. Its mechanism involves binding to receptors in the lateral hypothalamus to curb appetite and to those in the medial hypothalamus to induce satiety. Genetic variations within the LEP gene have been implicated in modulating circulating leptin concentrations and are associated with economically significant production characteristics, such as milk yield and quality.
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    SCIENTIFIC AND REGULATORY PERSPECTIVES ON IRRADIATED FOOD: DETECTION STANDARDS AND SAFETY EVALUATION
    (Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02)
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    Food irradiation is a scientifically validated and internationally endorsed technology that applies ionizing radiation to improve the microbiological safety and shelf life of food products. By effectively inactivating pathogens, parasites, and pests without significantly compromising nutritional or sensory quality, irradiation serves as a potent alternative to conventional decontamination methods. This review provides a comprehensive analysis of the scientific principles underlying irradiation, detailing the mechanisms of action, microbiological efficacy, toxicological safety, and minimal impact on nutritional value. These findings are supported by extensive evaluations from authoritative bodies, including the World Health Organization (WHO), the Food and Agriculture Organization (FAO), the International Atomic Energy Agency (IAEA), the European Food Safety Authority (EFSA), and the U.S. Food and Drug Administration (FDA). The paper also explores the regulatory landscape surrounding food irradiation, with particular focus on the European Union’s limited list of approved irradiated food categories under Directives 1999/2/EC and 1999/3/EC. The implications of regulatory fragmentation within the EU and the contrasts with countries such as the United States, Canada, India, and Thailand are assessed. Key applications, such as pathogen reduction in meat and poultry, insect control in spices and grains, and phytosanitary treatment for exportable fruits are discussed alongside economic and logistical benefits. Consumer perception remains a major barrier to widespread acceptance, often shaped by misconceptions and negative associations with the term “irradiation.” Strategies for effective communication, labeling transparency, and public education are examined. The review further assesses CEN (the European Committee for Standardization)-standardized and emerging detection methodologies critical for regulatory enforcement and traceability. Finally, it highlights the role of irradiation in advancing climateresilient food systems, reducing post-harvest losses, and supporting global food security, while calling for harmonized regulation and greater international cooperation.
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    ASSESSMENT OF RADIOACTIVITY AND RADIOLOGICAL HAZARD FROM NATURAL RADIONUCLIDES CONTAINED IN RICE FROM NORTH MACEDONIA
    (Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02)
    Angeleska, Aleksandra
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    Jovanov, Stefan
    Studies on radioactivity in consumable food are becoming increasingly important from a safety perspective as it is necessary to estimate the dose of ingestion by the public. For this reason, the focus of this study was on determining the activity concentrations of 226Ra, 232Th, and 40K. Thirty-eight rice samples were collected during 2024 from rice fields (city of Kochani) in the Republic of North Macedonia and the samples were analyzed by using a high-purity germanium (HPGe) detector for assessment of natural and artificial radioactivity. The average activity concentrations of 226Ra, 232Th, and 40K of the tested samples were 2.17±0.70, 1.78±0.72, and 48.48±1.92 Bq/kg, respectively. No artificial radionuclide was found in any of these samples. The total absorbed dose (D) was 4.10 D (nGy h-1), while the average value of the radium equivalent activity in all samples was 8.10 Bq kg-1, which was less than the maximum permitted value of 370 Bq kg-1. The values of Hex, Hin and 𝐼γ for the samples were 0.02, 0.027 and 0.064, which are less than one in all samples indicating the harmlessness of the samples. The average activity concentrations of 226Ra, 40K, and 232Th (Bq kg-1) in the samples were used to calculate the annual effective dose equivalent (AEDE), whose average value for all samples was 0.005(μSv/y). The average calculated cancer risk due to the intake of the tested radionuclides found in the rice samples was estimated at 0.017, which is within the range of acceptable risk values recommended by USEPA. The results of this study indicate that the intake of radionuclides due to consumption of the mentioned food does not have a harmful effect on public health. These data would be useful for establishing a baseline for the concentrations of natural radioactivity in food products consumed in the Republic of North Macedonia.
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    ASSESSMENT OF NATURAL RADIOACTIVITY LEVELS IN AGRICULTURAL SOIL AND TRANSFER IN RICE IN THE KOCHANI REGION, NORTH MACEDONIA
    (Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02)
    Angeleska, Aleksandra
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    Soil can transfer radionuclides to plants which are subsequently consumed by humans. Sometimes their levels are so high that they are a concern for human health. Rice has an important share in the Macedonian diet, especially in the Kochani region, which is famous for its rice fields. Therefore, the main goal of this study was to measure the natural radioactivity in agricultural soil and rice crops in the Kochani region and to study the interaction between soil and rice by calculating the transfer factor (TF). The activity concentrations of 226Ra, 232Th and 40K were determined by using gamma spectrometry, i.e. a high purity germanium (HPGe) gamma-ray spectrometer. In agricultural soils, the mean activity concentrations of 226Ra, 232Th and 40K were 33.72 Bq kg-1, 37.62 Bq kg-1 and 152.90 Bq kg-1, while the mean activity concentrations of rice were 1.99 Bq kg-1, 2.51 Bq kg-1and 49.44 Bq kg-1, respectively. The world’s mean soil values of 226Ra, 232Th and 40K are 35, 30 and 400 Bq kg-1, respectively, hence these obtained values are lower compared to those obtained from research. This may be due to improved agricultural practices in the country and the geology of the area itself. However, we found that the radioisotopes have a heterogeneous distribution which must be caused by the nature and soil types in the area for which this study was conducted. However, the radioisotopes showed a heterogeneous distribution, likely influenced by the nature and soil types in the region. The mean transfer factors (TF) were very low for all samples, indicating a slow transport of these radionuclides to the rice grains. The results confirm that rice grown in these areas is safe for consumption. Further research on the TF across different rice varieties and geographical areas would allow for the development of predictive models for potential radiation exposure from rice consumption and help identify locations requiring additional monitoring. All mean transfer factors (TF) for the samples were very low, implying a very slow transport of these radionuclides to the rice grains. The obtained results make rice grown in these areas safe for consumption. By studying the TF among different rice varieties and geographical areas, researchers will be able to develop models to predict possible radiation exposure from rice consumption and locate activities or areas that require additional attention.
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    Implementation of biosecurity measures in ruminants farms
    (Zbornik radova 26. medunarodni kongres Mediteranske federacije za zdravlje i produkciju preživara - FeMeSPRum, 2024-01)
    Branislav Stanković
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    Slavca Hristov
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    Dejan Bugarski
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    Milan Ninković
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    Branislav Kureljusic
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    EXAMINATION OF DRINKING WATER FOR ANIMALS AS A BIOSECURITY MEASURE ON DAIRY FARMS
    (34. SAVETOVANJE DEZINFEKCIJA, DEZINSEKCIJA I DERATIZACIJA JEDAN SVET – JEDNO ZDRAVLJE, 2023-06)
    Ena Dobric
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    Miroslav Kjosevski
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    Elena Mitrevska
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    Monika Dovenska
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    Comparison of the two different protocols for biosecurity assessment in commercial pig farms
    (9th International Scientific Meeting Days of Veterinary Medicine 2022, 2022-09)
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    Vanja Kondratenko
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    Milenko Simovikj
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    Jovan Aleksandar Bojkovski
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    Miroslav Kjosevski
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    Review of Legal Requirements on Ammonia and Greenhouse Gases Emissons from Animal Production Buildings in European countries
    (2019 ASABE Annual International Meeting, 2019-06)
    Bjarne Bjerg
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    Tomas Joseph Norton
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    et al.
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    Sensitivity of the integrated Welfare Quality® scores of the dairy cattle protocol to changes in individual measures
    (Benelux ISAE conference 2016, 2016-10)
    Sophie de Graaf
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    Bart Ampe
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    Stephanie Buijs
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    Sine N. Andreasen
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    Alice De Boyer des Roches
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    Seroprevalence of Toxoplasma gondii infection in farmed sows in the Republic of Macedonia
    (7th International Scientific Meeting - Days of Veterinary Medicine 2016At: Struga, Republic of Macedonia, 2016-07)
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