Faculty of Veterinary Medicine
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Item type:Publication, Depletion of [3H] amitraz in sheep(Springer Science and Business Media LLC, 2025-12-24); ;Jovanovski, Stefan ;Janevski, Aleksandar ;Musliu, Dea - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Monitoring Heavy Metals, Mycotoxins, Coccidiostats and Microbial Contamination in Animal Feed: A North Macedonia Study (2018-2022)(Walter de Gruyter GmbH, 2025-06-24); ; ;Enimiteva, Vangelica ;Angelovski, LjupcoFeed contamination can occur at every stage of the feed chain, including manufacturing, storage, transport, and utilization. Ensuring feed safety is fundamental for livestock health, animal performance, and food safety throughout the feed-to-food chain. The study aimed to make qualitative and quantitative assessment of microbiological and chemical hazards in animal feed in North Macedonia from 2018 to 2022. A total of 1,629 feed samples were analysed for heavy metals, mycotoxins, and coccidiostats, whereas 598 samples were examined for microbial contamination. The results indicate a significant prevalence of heavy metals, with lead (Pb) detected in 100% of the samples, although mostly within regulatory limits. Mycotoxins, notably ochratoxin A (OTA) and aflatoxin B1 (AFB1), were also identified, with non-compliance in 10.88% of cattle feed for AFB1. Coccidiostat residues exceeded the maximum levels in 4.6% of the samples. Microbiological analysis revealed that 1.34% of feed samples were contaminated with Salmonella spp., and 3.8% tested positive for sulphitereducing clostridia. The findings on both microbiological and chemical hazards indicate their potential to threaten the feed safety chain. Accordingly, this study emphasizes the need for continuous comprehensive feed safety monitoring and the enforcement of stringent safety regulations to safeguard animal and public health in North Macedonia. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Multi-class analysis of antimicrobial substances in poultry feed at cross-contamination levels by UHPLC-MS/MS – Method establishment, validation, and application(Society of Chemists and Technologists of Macedonia, 2025-12-24); ;Koceva, Dushica ;Angjeleska, Aleksandra ;Stojković, GoranThe Commission Delegated Regulation (EU) 2024/1229 establishes maximum limits (MLs) for the cross-contamination of antimicrobial substances in feed intended for food-producing animals. This study presents the development and in-house validation of two multi-class analytical methods for detecting antimicrobial substances in poultry feed at cross-contamination levels, using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Methods with and without solid-phase extraction (SPE) purification were developed for 26 antimicrobial substances across eight classes, covering a concentration range of 5–300 μg kg–1. The methods were validated for parameters in-cluding selectivity, limit of quantification, within- and between-day precision, accuracy, decision limit, matrix effect, and short-term analyte stability in the corresponding extracts. Both methods met the required performance criteria for detecting antimicrobial substances at cross-contamination levels, except for reduced sensitivity to lincomycin in the SPE method. Between-day precision was below 18 % and 19 %, with recoveries ranging from 95 to 103 %, for the method without SPE, and from 88 % to 101 % for the method with SPE, respectively. The analytes remained stable after two days of storage in the dark un-der two temperature conditions. These methods were applied to 39 poultry feed samples, revealing that approximately 41 % contained one or more antimicrobial substances, some of which exceeded the cross-contamination MLs. This study underscores the importance of simple, rapid, sensitive, and reliable analytical methods for controlling the presence of antimicrobials at cross-contamination levels. Such methods enable stringent control to prevent uncontrolled antimicrobial use in poultry production systems, thereby mitigating the development of antimicrobial resistance. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, RADIONUCLIDES IN THE FOOD CHAIN: FOCUS ON ANIMAL DERIVED FOOD(Goce Delchev University - Shtip, 2025-12-31) ;Angeleska, Aleksandra; ; ; The paper presents an analytical overview of the occurrence, movement, and accumulation of both natural and anthropogenic radionuclides in food of animal origin. The research encompasses the main pathways through which these substances enter animal organisms, together with the environmental and agricultural factors influencing their distribution in soil, vegetation, and animal tissues such as meat, milk, eggs, and fish during the period 1989 to 2025. The review was conducted based on a literature search performed in the Elsevier, Hinari, PubMed, and other relevant scientific databases. The study focuses on isotopes including uranium-238, thorium-232, potassium-40, cesium-137, and strontium-90 as key indicators of naturally occurring and technologically induced radioactivity within the biosphere. The analysis demonstrates the interconnection between geological, climatic, and agricultural conditions that determine the levels and variability of radionuclides in animal-derived food. It was established that radionuclide concentrations in North Macedonia fall within internationally accepted limits and correspond with values observed in other European countries. The study emphasizes the importance of continuous monitoring of both natural and anthropogenic sources of radioactivity to enable timely detection of fluctuations in radiological balance and to ensure the long-term safety of food and public health protection.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, SCIENTIFIC AND REGULATORY PERSPECTIVES ON IRRADIATED FOOD: DETECTION STANDARDS AND SAFETY EVALUATION(Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02); ; ; ; Food irradiation is a scientifically validated and internationally endorsed technology that applies ionizing radiation to improve the microbiological safety and shelf life of food products. By effectively inactivating pathogens, parasites, and pests without significantly compromising nutritional or sensory quality, irradiation serves as a potent alternative to conventional decontamination methods. This review provides a comprehensive analysis of the scientific principles underlying irradiation, detailing the mechanisms of action, microbiological efficacy, toxicological safety, and minimal impact on nutritional value. These findings are supported by extensive evaluations from authoritative bodies, including the World Health Organization (WHO), the Food and Agriculture Organization (FAO), the International Atomic Energy Agency (IAEA), the European Food Safety Authority (EFSA), and the U.S. Food and Drug Administration (FDA). The paper also explores the regulatory landscape surrounding food irradiation, with particular focus on the European Union’s limited list of approved irradiated food categories under Directives 1999/2/EC and 1999/3/EC. The implications of regulatory fragmentation within the EU and the contrasts with countries such as the United States, Canada, India, and Thailand are assessed. Key applications, such as pathogen reduction in meat and poultry, insect control in spices and grains, and phytosanitary treatment for exportable fruits are discussed alongside economic and logistical benefits. Consumer perception remains a major barrier to widespread acceptance, often shaped by misconceptions and negative associations with the term “irradiation.” Strategies for effective communication, labeling transparency, and public education are examined. The review further assesses CEN (the European Committee for Standardization)-standardized and emerging detection methodologies critical for regulatory enforcement and traceability. Finally, it highlights the role of irradiation in advancing climateresilient food systems, reducing post-harvest losses, and supporting global food security, while calling for harmonized regulation and greater international cooperation. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, ASSESSMENT OF RADIOACTIVITY AND RADIOLOGICAL HAZARD FROM NATURAL RADIONUCLIDES CONTAINED IN RICE FROM NORTH MACEDONIA(Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02) ;Angeleska, Aleksandra; ; ; Jovanov, StefanStudies on radioactivity in consumable food are becoming increasingly important from a safety perspective as it is necessary to estimate the dose of ingestion by the public. For this reason, the focus of this study was on determining the activity concentrations of 226Ra, 232Th, and 40K. Thirty-eight rice samples were collected during 2024 from rice fields (city of Kochani) in the Republic of North Macedonia and the samples were analyzed by using a high-purity germanium (HPGe) detector for assessment of natural and artificial radioactivity. The average activity concentrations of 226Ra, 232Th, and 40K of the tested samples were 2.17±0.70, 1.78±0.72, and 48.48±1.92 Bq/kg, respectively. No artificial radionuclide was found in any of these samples. The total absorbed dose (D) was 4.10 D (nGy h-1), while the average value of the radium equivalent activity in all samples was 8.10 Bq kg-1, which was less than the maximum permitted value of 370 Bq kg-1. The values of Hex, Hin and 𝐼γ for the samples were 0.02, 0.027 and 0.064, which are less than one in all samples indicating the harmlessness of the samples. The average activity concentrations of 226Ra, 40K, and 232Th (Bq kg-1) in the samples were used to calculate the annual effective dose equivalent (AEDE), whose average value for all samples was 0.005(μSv/y). The average calculated cancer risk due to the intake of the tested radionuclides found in the rice samples was estimated at 0.017, which is within the range of acceptable risk values recommended by USEPA. The results of this study indicate that the intake of radionuclides due to consumption of the mentioned food does not have a harmful effect on public health. These data would be useful for establishing a baseline for the concentrations of natural radioactivity in food products consumed in the Republic of North Macedonia. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, ASSESSMENT OF NATURAL RADIOACTIVITY LEVELS IN AGRICULTURAL SOIL AND TRANSFER IN RICE IN THE KOCHANI REGION, NORTH MACEDONIA(Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..., 2025-10-02) ;Angeleska, Aleksandra; ; ; Soil can transfer radionuclides to plants which are subsequently consumed by humans. Sometimes their levels are so high that they are a concern for human health. Rice has an important share in the Macedonian diet, especially in the Kochani region, which is famous for its rice fields. Therefore, the main goal of this study was to measure the natural radioactivity in agricultural soil and rice crops in the Kochani region and to study the interaction between soil and rice by calculating the transfer factor (TF). The activity concentrations of 226Ra, 232Th and 40K were determined by using gamma spectrometry, i.e. a high purity germanium (HPGe) gamma-ray spectrometer. In agricultural soils, the mean activity concentrations of 226Ra, 232Th and 40K were 33.72 Bq kg-1, 37.62 Bq kg-1 and 152.90 Bq kg-1, while the mean activity concentrations of rice were 1.99 Bq kg-1, 2.51 Bq kg-1and 49.44 Bq kg-1, respectively. The world’s mean soil values of 226Ra, 232Th and 40K are 35, 30 and 400 Bq kg-1, respectively, hence these obtained values are lower compared to those obtained from research. This may be due to improved agricultural practices in the country and the geology of the area itself. However, we found that the radioisotopes have a heterogeneous distribution which must be caused by the nature and soil types in the area for which this study was conducted. However, the radioisotopes showed a heterogeneous distribution, likely influenced by the nature and soil types in the region. The mean transfer factors (TF) were very low for all samples, indicating a slow transport of these radionuclides to the rice grains. The results confirm that rice grown in these areas is safe for consumption. Further research on the TF across different rice varieties and geographical areas would allow for the development of predictive models for potential radiation exposure from rice consumption and help identify locations requiring additional monitoring. All mean transfer factors (TF) for the samples were very low, implying a very slow transport of these radionuclides to the rice grains. The obtained results make rice grown in these areas safe for consumption. By studying the TF among different rice varieties and geographical areas, researchers will be able to develop models to predict possible radiation exposure from rice consumption and locate activities or areas that require additional attention. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Ionizing Radiation in Meat Processing and its Effects(VINČA INSTITUTE OF NUCLEAR SCIENCES, 2025-10) ;Angeleska, Aleksandra; ; ; From a historical perspective, even in ancient times there was an awareness of the connection between food and human health. Hippocrates (460 BC), the father of medicine, emphasized that many diseases could be linked to diet, highlighting the idea that “let food be thy medicine and medicine be thy food.” This early recognition of the importance of safe and high-quality nutrition laid the foundations for later efforts to preserve food and protect it from harmful influences. Over the centuries, various preservation methods were developed, ranging from drying, salting, and smoking to modern technologies that include ionizing radiation as an effective way of extending shelf life and maintaining the nutritional properties of meat. This paper provides a overview of ionizing radiation in meat processing. The advantages of this technology are evident in the elimination of pathogenic microorganisms, the extension of shelf life, and the preservation of nutritional properties, while acknowledging the limitations that may occur at higher doses. The analysis encompasses microbiological, chemical, physical, and sensory aspects, along with relevant regulatory frameworks at both international and national levels. It is concluded that ionizing radiation offers considerable potential for improving food safety and sustainability in the food industry, but requires careful application and transparent communication with consumers. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A SYSTEMATIC REVIEW OF THE IMPACT OF CLIMATE CHANGE ON AGRICULTURE AND FUTURE ADAPTATION STRATEGIES FOR NORTH MACEDONIA(Agrosym 2024, 2024-10-10); ; ; ; Angeleska, AleksandraClimate change poses significant challenges to agriculture worldwide, with implications for food security, livelihoods, and ecosystems. This review paper examines the impact of climate change on agriculture and explores adaptation strategies for North Macedonia. The paper synthesizes existing literature on the subject, drawing from peer-reviewed articles, academic papers, and reports. The introduction provides an overview of the interconnectedness between climate change and agriculture, emphasizing the vulnerability of the agricultural sector to changing climatic conditions. The materials and methods section outlines the methodology employed, including a systematic literature search, source selection criteria, data extraction, analysis, and synthesis. Results and discussion focus on five key areas: crop adaptation, water management, pests and diseases in crops, biodiversity, and agroforestry. Each subsection discusses the challenges posed by climate change in these areas and explores potential adaptation measures tailored to North Macedonian context. The conclusion highlights the urgency of addressing climate change impacts on agriculture and emphasizes the importance of adopting climate-smart agricultural practices. It underscores the need for collaborative efforts between stakeholders to promote knowledge exchange, capacity building, and policy interventions. This paper highlights the importance of adapting agricultural practices to mitigate the adverse effects of climate change on food production and rural livelihoods in North Macedonia. By embracing adaptation strategies, policymakers, farmers, and other stakeholders can enhance resilience and ensure sustainable agricultural development in the face of a changing climate. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, DEOXYNIVALONOL IN PIG FEED: AN OVERVIEW ON OCCURENCE IN THE PERIOD OF FIVE YEARS (2019-2024)(Faculty of Veterinary Medicine - Skopje, 2024-09-22); ;Dushica, Koceva ;Gordana, Ilievska ;Angeleska, AleksandraDeoxynivalenol (DON) belongs to the large group of mycotoxins called trichothecenes, occurring predominantly in cereal grains such as wheat, barley, oats, rye and maize. DON is primarily produced by the pathogenic Fusarium fungi which grow on the cereals in the field, preferably at temperate climates as they are commonly found in Europe. Cereal grains intended for food and feed may also become contaminated during storage. Deoxynivalenol can make animals refuse feed even at low doses, and at larger concentrations, it can cause vomiting, leading to growth depression, increase an animal's susceptibility to infections, cause diarrhea, and hemorrhage. DON also has been connected with the human disease ATA (alimentary toxic aleukia). DON is chemically stable and extent resistant to thermal processing. Because it is difficult to neutralize or remove DON from feed, it is important to detect contamination by these trichothecene mycotoxin. At least 40 countries have set maximum levels (MRLs) or guidelines for DON in animal feed on a global scale, ranging from 0.9 to 12 mg/kg. Total of 25 complementary feedingstuffs for fattening pigs, 28 feed samples for sows, 19 samples for piglets, 10 corn and 10 barley samples were analyzed in our laboratory for presence of DON. ELISA screening method was used for determination, which is validated (in compliance with 2002/657/EC) and accredited (ISO 17025). The results were evaluated according to Macedonian legislation (Official Gazette 53/2013) which are in accordance with European regulations (2006/576/EU). All cereal samples (corn and barley) were with DON concentration below LOD (45 µg/kg). Ten (10) out of 28 samples for sows (35.7%) were in concentration range of 65 – 898.74 µg/kg. DON was found in 6 out of 25 samples for fattening pigs (24%) in concentration range of 40.63 – 800 µg/kg. Both of these categories of pig feed fulfill the criteria set in the regulations (5 mg/kg for complementary and complete feedingstuffs). Concerning the feed for piglets, 6 samples out of 19 (31.5%) shown presence of DON in the concentration range of 77.3 – 143 µg/kg. MRL for pigs is set on 0.9 mg/kg. Although all feedingstuffs for pigs do not surpassed MRLs values proposed within the regulation, it is important to acknowledge the existence of DON, and mycotoxins in general, in feed samples. The strategies to be undertaken in the future should be aimed at prevention, elimination and detoxification, as three basic approaches in the fight against mycotoxins and their entry into food chain.
