Dodovski, Aleksandar
Preferred name
Dodovski, Aleksandar
Official Name
Dodovski, Aleksandar
Main Affiliation
Email
adodovski@fvm.ukim.edu.mk
14 results
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Item type:Publication, Isolation and molecular identification of Mycobacterium bovis and Mycobacterium caprae from slaughtered reactor cattle(Faculty of Veterinary medicine in Skopje, 2016-09); ; ; ; Angjelovski Branko - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Molecular survey of infectious bronchitis virus in commercial flocks in Macedonia reveals presence of different genotypes(COST - European Cooperation in science and technology, 2016-06); ; ; Introduction: Preliminary testing of commercial flocks using molecular and phylogenetic analysis revealed presence of genotypes QX and 4/91 in poultry farms with clinical signs and in poultry farms without clinical signs. Poultry farms are performing different vaccination programs without the knowledge of the genotype of the circulating strains. The purpose of this study was to detect presence of IBV in commercial flocks in Macedonia by molecular methods and to genotype the detected strains thus to establish a map of circulating genotypes in the country. Methods: Molecular survey was performed in 35 poultry farms representative for the whole territory of the country without clinical signs at different production age as part of active surveillance for avian influenza. From each farm 30 cloacal swabs were taken totalling 1050 samples. Samples were pooled by five in the laboratory. Additionally, samples (oropharyngeal swabs, affected organs) were taken from 15 farms with clinically signs (respiratory signs, drop in egg production, altered eqq quality) and post-mortem (respiratory, ovary and kidney lesions) findings associated to IBV. A real-time RT-PCR was performed targeting UTR region on all samples. A two-step nested RT-PCR was performed on all positive samples with primers targeting the S1 gene. Genotyping and subsequent phylogenetic analysis was performed by partial sequencing of the S1 gene region. Results: Results of the present study revealed presence of following genotypes of IBV’s circulating in commercial poultry in Macedonia: 4/91, Italy-02, QX, Mass, D274. This is first report of detection of Italy-02 in Macedonia. Conclusions: Establishing a map of circulating IBV genotypes will help towards better optimisation of vaccination protocol of poultry farms and will contribute towards better understanding of epidemiological situation on a country and regional level. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Application of Fluorescence Based Molecular Assays for Improved Detection and Typing of Brucella Strains in Clinical Samples(Faculty of Veterinary Medicine - Skopje, 2015-10-01); ; ; <jats:title>Abstract</jats:title> <jats:p> Bacteria from the genus Brucella are causative agents of brucellosis - a zoonotic disease which affects many wild and domestic animal species and humans. Taking into account the significant socio-economic and public health impact of brucellosis, its control is of great importance for endemic areas. The chosen control strategy could be successful only if adapted to the current epidemiological situation. This implies that a choice of appropriate diagnostic procedures for detection and typing of Brucella spp. strains are of essential importance. Significant advancement of molecular techniques and their advantages compared to classical methods, give strong arguments in promotion of these techniques as a powerful tool for comprehensive diagnostics of brucellosis. Considering this, the major tasks of the study were to select and implement molecular tests for detection and genotyping Brucella spp. and evaluate their performances using DNA from cultivated brucellae (islolates) and limited number of tissue samples from seropositive animals. The obtained results confirmed that implemented real time PCR for Brucella spp. detection, as well as MLVA-16 used for genotyping, have excellent analytical sensitivity (4.2 fg of Brucella DNA were successfully detected and genotyped). Furthermore, compared to bacteriological cultivation of Brucella spp., real time PCR and MLVA-16 protocols showed superior diagnostic sensitivity and detected Brucella DNA in tissues from which Brucella could not be cultivated. Based on the summarized study results, we propose a diagnostic algorithm for detection and genotyping of Brucella spp. bacteria. Routine use of proposed diagnostic algorithm will improve the effectiveness of infection confirmation and help for accurate evaluation of epidemiological situation.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Characterization and Epidemiology of Pigeon Paramyxovirus Type-1 Viruses (PPMV-1) Isolated in Macedonia(American Association of Avian Pathologists (AAAP), 2017-06); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Bovine Tuberculosis in the Republic of Macedonia: Postmortem, Microbiological and Molecular Study in Slaughtered Reactor Cattle(Walter de Gruyter GmbH, 2017-03-01); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Detection of Chlamydophilapsittaci in free-living birdsusing ELISA and Immunohistochemicalmethods(2006); ; ; ; D. Mitevski - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Profiling <i>Mycoplasma hyopneumoniae</i> Infection in Commercial Pig Farms Using Serology and Lung Lesions Assessment(Walter de Gruyter GmbH, 2023-05-06); Prodanovic, Radisa<jats:title>Abstract</jats:title> <jats:p> <jats:italic>Mycoplasma hyopneumoniae</jats:italic> (<jats:italic>Mhyo</jats:italic>) is primary pathogen involved in porcine respiratory diseases complex (PRDC). The objective of this study was to evaluate <jats:italic>Mycoplasma hyopneumoniae</jats:italic> (<jats:italic>Mhyo</jats:italic>) infection in five commercial pig farms by using serology and lung lesion scoring at slaughter. Five Macedonian pig herds with a history of respiratory diseases were included in the study. Blood samples were taken from pigs at 6, 10, 14, 18, and 22 weeks of age. Ten animals per age group from each farm were sampled and tested for antibodies to <jats:italic>Mhyo</jats:italic>. At abattoir, 50 lungs per farm were scored for lesions associated with enzootic pneumonia (EP). All farms were seropositive to <jats:italic>Mhyo</jats:italic>. Higher seroprevalence to <jats:italic>Mhyo</jats:italic> was observed in grower and finisher pigs, while significant difference (p<0.001) was detected among farms in 10-, 14-, and 22-week-old pigs. Enzootic pneumonia-like lesions were detected in 91.2% of all tested lungs with range of 82 to 98% on farm level. Mean lung lesion score (LLS) obtained for all farms was 11.5 (8.04–14.4). Significant difference for LLS and significantly higher percentage of severe LLS grade (>10) were found among some of the farms (p<0.001). In conclusion, high seroprevalence to <jats:italic>Mhyo</jats:italic> in finishing pigs in most of the farms was most likely due to vaccination. Farms with higher seroprevalence to <jats:italic>Mhyo</jats:italic> obtained lower LLS. Serology monitoring of different pig categories and lung assessment at slaughterhouse is a practical tool for assessing vaccine efficacy of <jats:italic>Mhyo</jats:italic> in pig farms.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, An Outbreak of Infectious Laryngotracheitis Virus in Commercial Layers: Three-Month Observation of Mortality, Virus and Antibody Dynamics(Walter de Gruyter GmbH, 2024-07-19); Savić, Vladimir<jats:title>Abstract</jats:title> <jats:p>Infectious laryngotracheitis (ILT) is a WOAH-listed respiratory disease in poultry caused by <jats:italic>Gallid alphaherpesvirus 1</jats:italic>, known as ILT virus (ILTV). We monitored two unvaccinated commercial layer flocks of 46- and 64-weeks old birds, more than 3 months after the onset of ILT. For this purpose, tracheal swabs, cloacal swabs, and blood samples were collected. Molecular and serology results were compared with the mortality data. The increased mortality in flocks 1 and 2 lasted 9 and 15 days, reaching 13.0% and 11.3%, respectively. We isolated the virus by inoculation on chicken embryo’s chorioallantoic membrane. Tracheal swabs were positive at each sampling point, but cloacal swabs were negative. Based on the molecular and phylogenetic analysis of the ICP4 gene, the ILTV closely matched vaccine strains. In flock 1, seroconversion was evident at the second sampling (day 15). Thereafter, an increase in antibody titer was observed, eventually achieving levels that were nearly identical to those on day 15 and on 109. During the acute period of the outbreak, seroconversion was already visible in flock 2, and a similar pattern was then seen as in flock 1. Three months after the outbreak, the virus DNA was still persistently detected in tracheal swabs.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First detection and biological characterization of an avian metaavulavirus 8 isolated from a migratory swan goose in Qinghai Lake, Northwest China(Wiley, 2024-12) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, The first report and biological characterization of Avian Orthoavulavirus 16 in wild migratory waterfowl and domestic poultry in China reveal a potential threat to birds(2025-02)