Characterization and Epidemiology of Pigeon Paramyxovirus Type-1 Viruses (PPMV-1) Isolated in Macedonia
Journal
Avian Diseases
Date Issued
2017-06
Author(s)
Naletoski, I.
Savić, Vladimir
DOI
10.1637/11517-101816-reg.1
Abstract
We have characterized in this study 10 PPMV-1 isolated from domestic pigeons and one PPMV-1 isolated from a
feral pigeon in the period 2007–2012, using both classical methods (HI test and ICPI test) and molecular methods (RT-qPCR,
RT-PCR, and nucleotide sequencing). Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with
recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. All isolates possessed virulent cleavage site motifs with
variable morbidity and mortality in pigeons. The intracerebral pathogenecity indices of the five isolates ranged from 0.59 to 1.53.
The repetitive isolation of PPMV-1 viruses for several consecutive years led toward establishing enzootic presence of the disease in
pigeons. A high nucleotide sequence homology between the Macedonian isolates and EU/re isolates was shown. Co-circulation of
different isolates in the same holdings was detected. This is the first study to extensively describe the molecular epidemiology of
PPMV-1 isolated in Macedonia.
feral pigeon in the period 2007–2012, using both classical methods (HI test and ICPI test) and molecular methods (RT-qPCR,
RT-PCR, and nucleotide sequencing). Using phylogenetic analysis of partial fusion gene sequences, these viruses clustered with
recent European PPMV-1 isolates (EU/re) within the genotype VIb/1. All isolates possessed virulent cleavage site motifs with
variable morbidity and mortality in pigeons. The intracerebral pathogenecity indices of the five isolates ranged from 0.59 to 1.53.
The repetitive isolation of PPMV-1 viruses for several consecutive years led toward establishing enzootic presence of the disease in
pigeons. A high nucleotide sequence homology between the Macedonian isolates and EU/re isolates was shown. Co-circulation of
different isolates in the same holdings was detected. This is the first study to extensively describe the molecular epidemiology of
PPMV-1 isolated in Macedonia.
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