Krstevski, Kiril
Preferred name
Krstevski, Kiril
Official Name
Krstevski, Kiril
Main Affiliation
Email
krstevski@fvm.ukim.edu.mk
35 results
Now showing 1 - 10 of 35
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Item type:Publication, Seroprevalence of Toxoplasma gondii infection in farmed saws in Republic of Macedonia(Faculty of Veterinary medicine in Skopje, 2016-09); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First reported cases of rabies in the Republic of Macedonia(BMJ, 2012-03-24); ; Dzadzovski, Igor - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Impact of measures to control brucellosis on disease characteristics in humans: experience from an endemic region in the Balkans(Informa UK Limited, 2018-05); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Molecular characterisation of rabies virus strains in the Republic of Macedonia(Springer Nature, 2013-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Isolation and molecular identification of Mycobacterium bovis and Mycobacterium caprae from slaughtered reactor cattle(Faculty of Veterinary medicine in Skopje, 2016-09); ; ; ; Angjelovski Branko - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Molecular survey of infectious bronchitis virus in commercial flocks in Macedonia reveals presence of different genotypes(COST - European Cooperation in science and technology, 2016-06); ; ; Introduction: Preliminary testing of commercial flocks using molecular and phylogenetic analysis revealed presence of genotypes QX and 4/91 in poultry farms with clinical signs and in poultry farms without clinical signs. Poultry farms are performing different vaccination programs without the knowledge of the genotype of the circulating strains. The purpose of this study was to detect presence of IBV in commercial flocks in Macedonia by molecular methods and to genotype the detected strains thus to establish a map of circulating genotypes in the country. Methods: Molecular survey was performed in 35 poultry farms representative for the whole territory of the country without clinical signs at different production age as part of active surveillance for avian influenza. From each farm 30 cloacal swabs were taken totalling 1050 samples. Samples were pooled by five in the laboratory. Additionally, samples (oropharyngeal swabs, affected organs) were taken from 15 farms with clinically signs (respiratory signs, drop in egg production, altered eqq quality) and post-mortem (respiratory, ovary and kidney lesions) findings associated to IBV. A real-time RT-PCR was performed targeting UTR region on all samples. A two-step nested RT-PCR was performed on all positive samples with primers targeting the S1 gene. Genotyping and subsequent phylogenetic analysis was performed by partial sequencing of the S1 gene region. Results: Results of the present study revealed presence of following genotypes of IBV’s circulating in commercial poultry in Macedonia: 4/91, Italy-02, QX, Mass, D274. This is first report of detection of Italy-02 in Macedonia. Conclusions: Establishing a map of circulating IBV genotypes will help towards better optimisation of vaccination protocol of poultry farms and will contribute towards better understanding of epidemiological situation on a country and regional level. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First comprehensive study on West Nile in the former Yugoslav Republic of Macedonia (FYROM)(EFSA, Parma, Italy, 2018-09); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, LABORATORY TESTS IN MONITORING HEALTH STATUS IN SHEEP FLOCKS(Serbian veterinary society, 2017-09); ; ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Application of Fluorescence Based Molecular Assays for Improved Detection and Typing of Brucella Strains in Clinical Samples(Faculty of Veterinary Medicine - Skopje, 2015-10-01); ; ; <jats:title>Abstract</jats:title> <jats:p> Bacteria from the genus Brucella are causative agents of brucellosis - a zoonotic disease which affects many wild and domestic animal species and humans. Taking into account the significant socio-economic and public health impact of brucellosis, its control is of great importance for endemic areas. The chosen control strategy could be successful only if adapted to the current epidemiological situation. This implies that a choice of appropriate diagnostic procedures for detection and typing of Brucella spp. strains are of essential importance. Significant advancement of molecular techniques and their advantages compared to classical methods, give strong arguments in promotion of these techniques as a powerful tool for comprehensive diagnostics of brucellosis. Considering this, the major tasks of the study were to select and implement molecular tests for detection and genotyping Brucella spp. and evaluate their performances using DNA from cultivated brucellae (islolates) and limited number of tissue samples from seropositive animals. The obtained results confirmed that implemented real time PCR for Brucella spp. detection, as well as MLVA-16 used for genotyping, have excellent analytical sensitivity (4.2 fg of Brucella DNA were successfully detected and genotyped). Furthermore, compared to bacteriological cultivation of Brucella spp., real time PCR and MLVA-16 protocols showed superior diagnostic sensitivity and detected Brucella DNA in tissues from which Brucella could not be cultivated. Based on the summarized study results, we propose a diagnostic algorithm for detection and genotyping of Brucella spp. bacteria. Routine use of proposed diagnostic algorithm will improve the effectiveness of infection confirmation and help for accurate evaluation of epidemiological situation.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Circulation of Crimean-Congo Hemorrhagic Fever Virus in the former Yugoslav Republic of Macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay(Public Library of Science (PLoS), 2015-03); ; There are only few assays available for the detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-specific antibodies in animals, and data about diagnostic sensitivity and specificity are incompletely documented for most of these tests. This is unfortunate since CCHFV antibodies in animals can be used as indicator for virus circulation in a geographic area and therewith potential risk of human exposure. This paper therefore reports on a novel ELISA for the detection of CCHFV-specific antibodies in cattle and on its application for testing ruminant sera from the Former Yugoslav Republic of Macedonia.