Faculty of Natural Sciences and Mathematics

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Lipoxygenase Inhibitory Activity and Prostate Cancer Cytotoxicity of In Situ- and In Vitro-Cultivated Balkan Endemic Sideritis scardica Griseb
(MDPI AG, 2025-10-25)
Danova, Kalina
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Stoyanova, Elena
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Alipieva, Kalina
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Sideritis scardica Griseb., a Balkan endemic species traditionally used for respiratory conditions and wound-healing, was investigated for its 15-lipoxygenase (15-LOX) inhibitory and cytotoxic activities in relation to its phenolic composition. Aerial parts from the wild-collected and in vitro-cultivated plant were successively extracted with hexane, chloroform, and methanol and the methanol extract was further fractionated into ethyl acetate, butanol, and water fractions. This study presents the first combined evaluation of LOX inhibition and cytotoxicity against prostate cell lines WPMY-1 (normal epithelial fibroblast/myofibroblast), PC-3 (epithelial adenocarcinoma, Grade IV), and LNCaP (epithelial carcinoma) and detailed phytochemical profiles of wild-collected and in vitro-cultivated S. scardica. The phytochemical analysis revealed distinct profiles: increased LOX-inhibition activity was related to a higher phenylethanoid/flavone glycoside ratio, while PC cytotoxicity was rather related to flavone glycoside domination in the plant preparations. The cytotoxic effect of the in vitro-obtained preparations was characterized by a marked selectivity when comparing their effects on WPMY-1, PC-3 and LNCaP cells. To our knowledge, this is the first report linking phenylethanoid/flavone glycoside profiles of in situ and in vitro S. scardica plants with dual LOX-inhibitory and anti-prostate cancer activities, supporting the plant’s potential as a sustainable source of bioactive compounds.
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LC-HRMS and NMR study of the esterification products of ibuprofen with solketal: Formation, isolation and identification
(Society of Chemists and Technologists of Macedonia, 2025-12-24)
Jakimovska Pokupec, Viktorija
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Popova, Milena
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Bankova, Vassya
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Ibuprofen is a widely used non-steroidal anti-inflammatory drug dispensed in tablets, capsules, suspensions, oral solutions, creams, and gels. Ibuprofen's poor water solubility and gastrointestinal side-effects present ongoing formulation challenges. Alcoholic excipients are often employed to enhance solubility and minimise adverse effects. Solketal (1,2-isopropylidene glycerol), a ketal produced by the condensation of glycerol with acetone, offers further versatility as an excipient due to its free hydroxyl group, which enables esterification reactions with acidic active pharmaceutical ingredients like ibuprofen. Introducing any excipient, especially in direct contact with the active pharmaceutical ingredient, necessitates careful evaluation of potential drug–excipient interactions, as these can alter the drug's physicochemical properties and impact clinical performance. Chromatographic techniques coupled with mass spectrometry and nuclear magnetic resonance spectroscopy remain essential for identifying and characterising related and degradation products in pre-formulation studies. In this study, we investigated the esterification of ibuprofen with solketal to identify possible interaction products. Two major compounds were isolated and thoroughly characterised by MS and NMR, confirming their chemical structures: 1-mono-glycerol ester of ibuprofen and ibuprofen-solketal-ester, which contained a 1,3-dioxolane ring. This finding highlights the importance of comprehensive analytical evaluation of drug–excipient interactions during formulation development, as these can affect drug stability and performance.
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Passive sampling-based characterization of volatile organic compounds in Skopje: seasonal trends and source identification
(Springer Science and Business Media LLC, 2025-10-27)
Sofronievska, Ivona
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Sofronievski, Bojan
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Establishment, evaluation, and application of a gas chromatography–mass spectrometry method for quantification of volatile organic compounds in ambient air using anisole as an internal standard
(SHTM, UKIM, 2024)
Sofronievska, Ivona
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In Vitro and in Silico Insights on the Biological Activities, Phenolic Compounds Composition of Hypericum perforatum L. Hairy Root Cultures
(Computers, Materials and Continua (Tech Science Press), 2023)
Tusevski, Oliver
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Petreska Stanoeva, Jasmina
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Gadzovska Simic, Sonja
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In Vitro and in Silico Insights on the Biological Activities, Phenolic Compounds Composition of Hypericum perforatum L. Hairy Root Cultures
(Computers, Materials and Continua (Tech Science Press), 2023)
Tusevski, Oliver
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Petreska Stanoeva, Jasmina
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Gadzovska Simic, Sonja
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Phenolic fingerprint of Macedonian propolis
(Society of Chemists and Technologists of Macedonia, 2024-05-19)
Petreska Stanoeva, Jasmina
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Stojchevski, Cvetan
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Bankova, Vassya
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Propolis is a chemically complex resinous material collected by honeybees (Apis mellifera) from tree buds and resins, comprising plant exudates, secreted substances from bee metabolism, pollen and waxes. Its chemical composition depends strongly on the plant sources available around the beehive, which have a direct impact оn the quality and bioactivity of the propolis. In this study, the composition of phenolic compounds in 13 Macedonian propolis extracts was investigated by HPLC-DAD-ESI-MS/MS. Overall, the UV spectra, the MS and MS/MS data allowed the identification of 36 compounds. The major constituents of propolis were phenolic acids (caffeic and coumaric) and their esters (methyl, (iso)prenyl, benzyl, phenylethyl, cinnamyl), flavonols (quercetin, kaemferol), flavones (chrysin, apigenin, acacetin), flavanonols (pinobanksin) flavanones (pinocembrin naringenin, hesperetin, pinostrobin) and their methylated/esterified derivatives. The results reveal that Macedonian propolis contains a diversity of phenolic compounds confirming that it is a poplar type of propolis with higher phenolic content (ranging from 43.75–637.94 mg/g) than reported in previous studies in the region and beyond in Europe (< 80 mg/g). This suggests the potential significance of Macedonian propolis as a valuable source of bioactive compounds with health benefits as well as for unlocking its economic potential for industry and beekeepers.
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Anthocyanin fingerprinting as an authentication testing tool for blueberry, aronia, and pomegranate juices
(Springer Science and Business Media LLC, 2023-12-19)
Petreska Stanoeva, Jasmina
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Damjanovski, Viktor
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Cichna-Markl, Margit
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UHPLC-Q-TOF analysis of pyrrolizidine alkaloids in North-Macedonian honey
(Informa UK Limited, 2023-10-26)
Cvetanoska, Marinela
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Pocrnić, Marijana
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Galić, Nives
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Petreska Stanoeva, Jasmina
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State of antioxidant systems and phenolic compounds’ production in Hypericum perforatum L. hairy roots
(Springer Science and Business Media LLC, 2019-07-09)
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Spasenoski, Mirko
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Gadzovska Simic, Sonja

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