Faculty of Veterinary Medicine

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    Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV
    (MDPI AG, 2021-06-06)
    Vidanović, Dejan
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    Tešović, Bojana
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    Šekler, Milanko
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    Debeljak, Zoran
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    Vasković, Nikola
    <jats:p>Lumpy skin disease (LSD) is an important animal disease with significant health and economic impacts. It is considered a notifiable disease by the OIE. Attenuated strains of LSDV have been successfully used as vaccines (LAV) but can also produce mild or systemic reactions. Vaccination campaigns using LAVs are therefore only viable if accompanying DIVA assays are available. Two DIVA qPCR assays able to distinguish Neethling-based LAVs and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic performance comparable to other published DIVA assays. This confirmed their potential as reliable tools to confirm infection in animals during vaccination campaigns based on Neethling vaccine strains.</jats:p>
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    Detection of Enterotoxigenic Potential of <i>Staphylococcus Aureus</i> Isolates from Cheese Samples with Two Different Methods
    (Macedonian Veterinary Review, 2022-03-29)
    Manovska, Marija Ratkova
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    Prodanov, Mirko
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    <jats:title>Abstract</jats:title> <jats:p>The primary objective of our study was to detect the occurrence of enterotoxigenic <jats:italic>Staphylococcus aureus</jats:italic> in diverse types of cheese (cow’s milk cheese and mixed milk cheese) samples from R.N. Macedonia. Cheese samples were analyzed for enumeration and isolation of the <jats:italic>S. aureus</jats:italic> strains according to ISO 6888-1. We detected the toxigenic potential of the strains by the use of the Enzyme Link Fluorescent Assay VIDAS system, and we confirmed the presence of the SEs (<jats:italic>sea, seb, sec, sed, see</jats:italic>) genes by multiplex PCR. The results showed that out of 270 samples of cheese, coagulase-positive staphylococci (CPS) were detected in 27 (10%), and coagulase-negative staphylococci in five samples (1.8%). Biochemically, all 27 CPS samples were confirmed to be <jats:italic>Staphylococcus aureus.</jats:italic> With VIDAS SET2 test we confirmed that 11 isolates are producers of one of the toxins limited by the test. With the conventional PCR we confirmed genes in only 7 isolates. Most common detected gene was <jats:italic>seb</jats:italic> n=3 (42.8%), followed by <jats:italic>sea</jats:italic> n=2 (28.6%), and <jats:italic>sec</jats:italic> n=2 (28.6%). Additionally, <jats:italic>sed</jats:italic> and <jats:italic>see</jats:italic> genes were not detected in any of the <jats:italic>S. aureus</jats:italic> isolates. Discrepancies between the two test methods for detection of enterotoxigenic potential are not uncommon. The presence of viable <jats:italic>Staphylococcus aureus</jats:italic> cells that have enterotoxin potency demonstrates the importance of appropriate hygiene practices in the diary process and also the maintenance of the products in order to obtain a safe final product for the consumers.</jats:p>