Faculty of Veterinary Medicine

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    Item type:Publication,
    Systematic multi-year surveillance of honey compliance and fraud in North Macedonia, 2020–2024
    (Springer Nature, 2026-02-20)
    Nestorovski Tome
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    Jovanov Stefan
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    Trajkovska Melita J.
    Honey adulteration represents a significant food safety and economic concern globally, yet comprehensive data from North Macedonia remains lacking. This study evaluated 538 honey samples (391 domestic, 147 imported) collected between 2020 and 2024 for compliance with national and EU quality standards. Overall, 9.48% of samples failed at least one quality parameter, with domestic honey showing higher non-compliance (12.53%) than imported (1.4%). The primary causes of non-compliance were elevated hydroxymethylfurfural (64.7%) and reduced diastase activity (43.1%), indicating heat-related exposure. Among 16 samples failing C4 sugar analysis, 7 (43.8%) met all other regulatory requirements, suggesting sophisticated adulteration methods that evade routine testing. Statistical analysis revealed very strong associations between heat-related parameters. Temporal analysis showed declining overall non-compliance but increasing prevalence of diastase-related failures. These findings, representing the first systematic assessment of honey adulteration in North Macedonia, indicate that while basic market surveillance appears effective, current testing protocols may miss economically motivated adulteration. Implementation of routine C4 sugar analysis, particularly for samples passing conventional parameters, is essential for comprehensive fraud detection and consumer protection.
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    The impact of the mineral zeolite on feed conversion and mortality rate on commercially grown rainbow trout
    (Institute of Animal and Fishery Science, 2025-11-10)
    Bodan Gjorgovski
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    Rodne Nastova-Gjorgioska
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    The aim of this study was to evaluate the effect of zeolite, added to feed, on feed conversion and mortality rate in commercially farmed rainbow trout. The research was conducted at the Riboexport DOOEL pond in Gostivar, at the Vrutok source. Three pools were included in the study: Group A – without zeolite, Group B – feed with 1 % zeolite, and Group C – feed with 2 % zeolite. The fish were reared under standardized conditions (length 4 m, width 1 m, depth 45 cm, total water volume 1.8 m³, temperature 11 °C, and oxygen concentration 9–11 mg/L). Each pool contained 200 juvenile rainbow trout with an initial weight of 46–48 g and length of 15–16 cm, monitored over a 12-week period. Measurements were taken 8 times, at 14-day intervals. Feed conversion was determined using the Feed Conversion Ratio (FCR), and mortality was recorded numerically. The results are presented using descriptive statistics, showing FCR values of 0.89 for Group A, 0.84 for Group B, and 0.89 for Group C. No mortality was recorded in Groups A and B (0 %), while 3 dead fish (1.5 %) were observed in Group C. The best feed conversion was observed in the group receiving feed with 1 % zeolite. Fish mortality was not related to zeolite application but was attributed to mechanical injuries during manual handling.
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    Item type:Publication,
    RADIONUCLIDES IN THE FOOD CHAIN: FOCUS ON ANIMAL DERIVED FOOD
    (Goce Delchev University - Shtip, 2025-12-31)
    Angeleska, Aleksandra
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    The paper presents an analytical overview of the occurrence, movement, and accumulation of both natural and anthropogenic radionuclides in food of animal origin. The research encompasses the main pathways through which these substances enter animal organisms, together with the environmental and agricultural factors influencing their distribution in soil, vegetation, and animal tissues such as meat, milk, eggs, and fish during the period 1989 to 2025. The review was conducted based on a literature search performed in the Elsevier, Hinari, PubMed, and other relevant scientific databases. The study focuses on isotopes including uranium-238, thorium-232, potassium-40, cesium-137, and strontium-90 as key indicators of naturally occurring and technologically induced radioactivity within the biosphere. The analysis demonstrates the interconnection between geological, climatic, and agricultural conditions that determine the levels and variability of radionuclides in animal-derived food. It was established that radionuclide concentrations in North Macedonia fall within internationally accepted limits and correspond with values observed in other European countries. The study emphasizes the importance of continuous monitoring of both natural and anthropogenic sources of radioactivity to enable timely detection of fluctuations in radiological balance and to ensure the long-term safety of food and public health protection.</jats:p>
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    Genetic characterisation of Echinococcus granulosus sensu lato infecting ruminants in the Republic of North Macedonia
    (2025-12)
    Rashikj, LJubica
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    Zagorka Popova Hristovska
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    Betim Xhekaj
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    Sand fly-associated phlebovirus with evidence of neutralizing antibodies in humans and dogs in Kosovo
    (Informa UK Limited, 2026-01)
    Kurum, Elif
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    Jakupi, Xhevat
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    Xhekaj, Betim
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    Platzgummer, Katharina
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    Hoxha, Ina
    The Balkan Peninsula is a hotspot for sand fly-borne phleboviruses (SbPVs), yet Kosovo had no confirmed viral detection invectors despite serological evidence of human and animal exposure. This study reports the discovery, genetic characterization, and seroprevalence of a novel phlebovirus, Grapi virus (GRPV), in Kosovo. Entomological surveys (2022-2023) collected 3,575 sand flies across seven districts. Morphological and molecular identification revealed Phlebotomus perfiliewi as the dominant species. Pan-phlebovirus RT-PCR screening identified GRPV in seven pools. Complete genome sequencing confirmed its tripartite genome, sharing 97.55-98.70% nucleotide identity with Bregalaka virus, classifying it within the Phlebovirus adanaense species. Phylogenetic analysis revealed segment-specific ancestry, suggesting recombination events between Bregalaka virus, Adana virus, and Medjerda Valley virus. Seroprevalence studies using neutralization assays detected GRPV-specific antibodies in 13.0% of humans and 2.7% of dogs. Human seropositivity peaked in adolescents and declined with age, while dogs showed higher rates in purebred and unhealthy ones. No cross-reactivity with Toscana or Sicilian viruses was observed, indicating distinct immunological responses. GRPV replicated efficiently in Vero cells and more slowly in mosquito cells, suggesting mammalian adaptation. GRPV detection in Kosovo underscores the role of the Balkan region in SbPV emergence. GRPV zoonotic potential is supported by the anthropophilic feeding behaviour of Ph. perfiliewi and by the significant seroprevalence rates in dogs and humans. Limitations include biased human/dog sampling and sparse northern Kosovo coverage. Investigating GRPV pathogenicity and ecology; integrated surveillance and diagnostics are essential for the future.
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    Performances of oxygenation devices on trout farms in Republic of Serbia, Republic of North Macedonia, and Bosnia and Herzegovina
    (Centre for Evaluation in Education and Science (CEON/CEES), 2025)
    Čanak, Stevan
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    Radosavljević, Vladimir
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    Gligorević, Kosta
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    <jats:p>Dissolved oxygen is universally recognized as the most decisive limiting factor in intensive trout aquaculture, exerting profound effects not only on production efficiency but also on fish welfare, physiological stability, immune competence, and the capacity to resist disease. When oxygen levels fall below optimal thresholds, trout are exposed to chronic stress, impaired metabolic processes, reduced feed intake, and a heightened susceptibility to pathogens, all of which compromise both productivity and sustainability. To counter these risks, water oxygenation—defined as the technological process of enriching aquaculture water with pure oxygen—has become an indispensable intervention for modern trout farming. In Republic of Serbia and Republic of North Macedonia, oxygenation systems have been continuously applied for more than two decades, while in Bosnia and Herzegovina their use has been established for over ten years, collectively enabling production intensification, seasonal stability, and improved economic outcomes. This study explores the diversity of oxygenation technologies currently employed, ranging from diffuser systems and low‑head oxygenators to pressurized tanks and devices with rotating mechanical components. Particular emphasis is placed on their operational principles, efficiency parameters, and exploitation characteristics, with attention to how these technologies are adapted to the specific environmental conditions and management practices of rainbow trout farms in the region.</jats:p>
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    Stats of fisheries and disease control in the Republic of North Macedonia in the erly 21th centery
    (Balkan Scientific Association of Agricultural Economists, 2022-07-30)
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    This review aims to show the current state of fisheries in the Republic of Northern Macedonia as well as the strategy for the prevention and control of World Organization for Animal Health (OIE) notifiable diseases in aquaculture in order to comply with European legislation. At the moment, Macedonia produces about 1,700 tons, which dominates the production of trout with 63% and carp with about 29% of the total production. This sector has had a constant. Positive growth in recent years, attracting new investors, especially in cold-water aquaculture. Although the consummation of 5 kg per capita of fish is modest, domestic production does not satisfy their needs, so the market is supplemented with fish imports. One of the conditions for this sector to continue to grow at such a pace is to limit losses in aquaculture production. The most serious limitation that serious producers deal with is the appearance of infectious diseases.
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    HONEY BEE HEALTH STATUS IN BULGARIA AND MACEDONIA, EVALUATION OF RISK
    (Institute of Knowledge Management, Skopje, 2017-03-31)
    Valtchovski Rumen
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    Hristovska Galina
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    Valchovski Petar
    Abstract: Today it is well known that beekeeping occupies one of the most important stakes in global agriculture.Besides the obvious contribution to the receipt of bee products (honey, pollen, wax, propolis and etc.), bees are one of the most effective natural pollinators of a wide variety of wild flora and crops. This activity led to thepreservation of biodiversity and improving the balance of the ecosystem. In the world as a whole the total economicvalue of pollination and bee products from beehives worth over 150 million euros a year (Gallai et al., 2008). To besuccessful this activity is particularly important to their health status. The mysterious mass extinction of bees, for which destroyed about a third of bee colonies every year since 2006, may be due in addition to the well-known Paenobacillus larvae and P. alvei, nosema, also of not a harmless part of viruses. Recent studies show an increase in mortality in winter, the 2016 and 2017, the and led to concerns about the continuation of the downward trend of bee population. We made a risk analysis of economically significant diseases in bees in Bulgaria and Macedonia. We believe that such analyzes have been made ahead of time for them this presents various issues related to the changed picture of the health of bees.
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    Antioxidant Potential of Pollen Polyphenols in Mitigating Environmental Stress in Honeybees (Apis mellifera)
    (MDPI AG, 2025-09-05)
    Tlak Gajger, Ivana
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    Honeybee populations are increasingly threatened by various environmental stressors, including pesticides, pathogens, and climate change. Emerging research highlights the vital role of pollen polyphenols in supporting honeybee health through a network of antioxidants, immune responses, and detoxification mechanisms. This review synthesizes current findings on the chemical diversity, bioactivity, and functional relevance of polyphenolic compounds in honeybee nutrition. Pollen polyphenols, which include flavonoids and phenolic acids, possess remarkably high antioxidant potential, up to 235 times greater than that of nectar. They also significantly increase the expression of antioxidant enzymes, immune system genes, and detoxification pathways such as cytochrome P450s and glutathione-S-transferases. These compounds also demonstrate antimicrobial effects against key pathogens and mitigate the toxic effects of pesticides. The content and composition of polyphenols vary seasonally and geographically, impacting the resilience of honeybee colonies. Field and laboratory studies confirm that polyphenol-rich diets improve survival, gland development, and stress resistance. Advanced analytical techniques, including metabolomics, have expanded our understanding of polyphenol profiles and their effects on honeybee physiology. However, knowledge gaps remain in pharmacokinetics and structure–function relationships. Integrating this evidence into conservation strategies and good beekeeping practices, such as habitat diversification and targeted feed supplementation, is crucial for maintaining honeybee health and ecosystem services in a rapidly changing environment.
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    Extended-Spectrum β-Lactamase-/AmpC-Producing Escherichia coli and Associated Risk Factors in Shelter Dogs: A Baseline Study in North Macedonia
    (MDPI AG, 2025-09-17)
    Shikoska, Ivana
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    Duvnjak, Sanja
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    Koritnik, Tom
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    Chapkunovska, Bojana
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    Vlahov, Jane
    Antimicrobial resistance (AMR) is a significant public health concern in companion animals, yet systematic surveillance in North Macedonia is lacking. This study investigated the prevalence of resistance in Escherichia coli isolated from 112 fecal samples from dogs in six shelters in North Macedonia and evaluated the associated risk factors, providing the first baseline dataset for this population. High resistance was observed for sulfamethoxazole (68.75%), ampicillin (52.68%), and ciprofloxacin (41.07%). Multidrug resistance was present in 50% of the isolates, with 17 (15.17%) confirmed as ESBL producers. Additionally, 18 isolates (16.1%) were identified as AmpC producers, 16 of which carried the blaCMY-2 gene. Notably, 72.2% of ESBL/AmpC isolates were resistant to ertapenem despite the absence of carbapenemase genes, a finding that warrants further investigation. Risk factors such as shared housing, longer shelter stays, and frequent empirical antimicrobial use were identified as probable contributors to the carriage of ESBL-/AmpC-producing E. coli. None of the shelters had antimicrobial stewardship protocols or routine diagnostic testing, revealing critical gaps in infection control and antimicrobial practices. These findings underscore the urgent need for antimicrobial stewardship and surveillance in North Macedonia’s companion animal populations within the One Health framework.