Faculty of Veterinary Medicine

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    Comparative analysis of conjugated linoleic acid content in cow, sheep and goat milk
    (Faculty of Food Technology Osijek (University of Osijek) and Faculty of Technology (University of Tuzla), 2019-10-24)
    Arapcheska, Mila
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    Tuteska, Jovanka
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    In recent years there has been increased interest in research on conjugated linoleic acid (CLA) and its potential health benefits: anticarcinogenic, antiatherogenic, antidiabetic and immunomodulatory effects. Term CLA describes a group of positional and geometric isomers of linoleic acid characterized by conjugated system of double bonds, separated by one single bond. The objective of this study was to analyze the differences in fatty acid (FA) profiles in cow, sheep and goat milk with an emphasis on the content of CLA. Milk samples were collected at conventional cow, sheep and goat farms, and according milk type were grouped in three groups. FA profiles were analyzed by gas chromatography. FAs were analyzed as methyl esters (FAMEs), and identified by comparison with methyl esters of standards. According to obtained results, significant difference (p<0.05) in CLA content was noted between three groups of samples, and also considerable individual variations in CLA content were noted inside each of group. Sheep milk samples were richer in CLA compared to goat and cow’s milk samples. Milk fat is the richest natural dietary source of CLA, and its content can be increased by manipulation of feeding regimes and genetic selection of dairy animals.
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    Comparative analysis of fatty acids composition between grass and lucerne forage
    (Trakya University, 2019-11-07)
    Arapcheska, Mila
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    Tuteska, Jovanka
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    Forages represent a major part of ruminant diets. Fatty acids composition of forage feed has major influence on energy intake and productivity of animals and also has significant effects on nutritional quality of milk and meat. The fatty acids composition of forages depends on many factors, including: species, variety, climate, growth stage, growth period, fertilization and conservation method. The aim of this study was to perform comparative analysis of fatty acid composition between grass and lucerne forage. The reliability and accuracy of the analytical method for the detection of fatty acids were ensured by use of the certified reference matrix that consisted of FAME standards of C14:0; C14:1; C16:0; C16:1; C18:0; C18:1; C18:2; C18:3 and C20:4, purchased form Supelco, Sigma-Aldrich. The content of the particular component was expressed as percentage from the sum of all analyzed fatty acids. According obtained results from analysis of fatty acid profile of feed samples higher content of saturated fatty acids was noted in samples of grass forage (41,87 %), compared with lucerne (31,24 %). The contents of monounsaturated fatty acids in samples of grass forage was 11,28 % and 11,08 % in samples of lucerne forage, while contents of polyunsaturated fatty acids in samples of grass forage was 33,59 % and 43,80 % in samples of lucerne forage. The contents of unsaturated fatty acids in meat and milk depend of their contents in animal feed. Forage feeding is main approach to increase the supply of beneficial fatty acids from plants into ruminant products. Analysis of fatty acids profiles of grass and legume forage is very important because of their effect on nutritional quality of and health benefits of ruminant products.
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    Gas chromatography analysis of omega-3 fatty acids content in fish oil supplements
    (Faculty of Agriculture Goce Delcev University - Stip, North Macedonia, 2019-04-12)
    Arapcheska, Mila
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    Tuteska, Jovanka
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    Jovanovska, Vangelica
    Fish oils are excellence source of omega-3 polyunsaturated fatty acids. Two main bioactive components responsible for health benefits of fish oil are omega-3 fatty acids: eicosapentaenoic (EPA) and docosahexaenoic (DHA), which are essential for humans as cannot be synthesized by the human body. They have been shown to lower risks of cardiovascular disease and reduce inflammation. Fish oil dietary supplements are widely available at various health food stores and pharmacies. The main objective of this study was to analyze omega-3 fatty acids content in encapsulated fish oil dietary supplements present at local market. Samples of four commercial types of encapsulated fish oil products sold on the local market were analysed by gas chromatography (GC-FID). The content of their eicosapentanoic acid (EPA) and docosahexaenoic (DHA) content was examined. The obtained values for EPA and DHA content in analyzed samples were lower than product declared values ranging from 6.06% to 10% for EPA and from 0.45% to 8% for DHA. These results indicate that the label claims for EPA and DHA for analyzed encapsulated fish oil products are presented with reasonable accuracy.
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    Item type:Publication,
    Health benefits of conjugated linoleic acid
    (Trakya University, 2019-11-07)
    Arapcheska, Mila
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    Tuteska, Jovanka
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    In recent years, the bioactive compounds present in food, even though in minor amounts, have received increased attention because they may have an important nutritional role. Conjugated linoleic acid (CLA) is a generic term used to describe a group of geometric and positional isomers of linoleic acid with a conjugated double bond mainly at carbons 9 and 11 or 10 and 12. Milk fat is the richest natural source of CLA. The CLA-isomers are produced via biohydrogenation of the unsaturated fatty acids presented in animal feed into saturated by rumen bacteria and by the enzymatic activity of the Δ9-desaturase in the mammary gland. Numerous physiological properties have been attributed to CLA including action as an antiadipogenic, antidiabetogenic, anticarcinogenic, antiatherosclerotic agent and chronic inflammatory diseases. In addition, CLA has effects on bone formation and the immune system as well as fatty acid and lipid metabolism and gene expression in numerous tissues. Because potential health benefits have been associated with dietary consumption of CLA, enhancement of CLA concentrations in meat and milk is an important objective in nutrition research.
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    Item type:Publication,
    Animal Stress as a Risk Factor in Animal Rescuing Operations
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, University of Ljubljana, Veterinary Faculty and Harran University, 2017-07-08)
    Martin Nikolovski
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    Miroslav Radeski
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    Dean Jankuloski
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    Investigetion of the cultivation influence on the levels of natural radionucleides in soil from the surrounding of the city of Skopje.
    (Society of physicists of Macedonia DFRM, 2017-04-28)
    Aleksandra Angjeleska
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    Elizabeta Dimitrieska-Stojkovikj
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    Biljana Stojanovska-Dimzoska
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    Item type:Publication,
    Evaluation of dose for the population due to natural radioactivity of uncultivated soil from the surrounding of the city of Skopje
    (BENA, 2016)
    Aleksandra Angjeleska
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    Elizabeta Dimitrieska-Stojkovikj
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    Bosko Boskovski
    Determination of natural radionuclides in the environment is important as relevant parameter for radiological risks estimations. The objective of this research was focused to define the activity concentrations of 226Ra, 232Th, and 40K in samples of uncultivated soil, taken from 13 locations in the surrounding of the Skopje city. Within the research performed, the obtained data indicated that the value of 232Th and 226Ra activity was within the range from 27.06 to 43.66 Bq kg–1, and from 20.38 to 39.44 Bq kg–1, respectively. As expected, the 40K concentration in soil was significantly higher, ranged between 392.48 and 625.80 Bq kg–1. The concentrations obtained were the basis for calculation of other parameters that are fundamental for determination of the environmental radiation safety for the population. The obtained results from this study suggest that in the surrounding of Skopje there is no significant radiation risk for the population, i.e. the safe limits were not exceeded, pointing out the insignificant risk that arises from naturally occurring radionuclides in the environmental soil.
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    Aflatoxins occurrence in feeding stuffs for poultry
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, 2016-09-24)
    Katerina Davceva
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    Biljana Stojanovska Dimzoska
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    Elizabeta Dimitrieska-Stojkovikj
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    Item type:Publication,
    Two year post incidence survey on the aflatoxin M1 occurrence in raw milk from the Republic of Macedonia
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, 2016-09-24)
    Elizabeta Dimitrieska-Stojkovikj
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    Gordana Arsova
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    Goran Stojkovikj
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    Biljana Stojanovska-Dimzoska
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    Aleksandra Angjeleska
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    Item type:Publication,
    Mycotoxin contamination in complete feeding stuffs for dairy cattle
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, 2016-09-24)
    Biljana Stojanovska-Dimzoska
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    Elizabeta Dimitrieska-Stojkovikj
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    Aleksandra Angjeleska