Cekovska, ZHaklina
Preferred name
Cekovska, ZHaklina
Official Name
Cekovska, ZHaklina
Main Affiliation
Email
zaklina.cekovska@medf.ukim.edu.mk
27 results
Now showing 1 - 10 of 27
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Item type:Publication, COMPARISON OF THE SUSCEPTIBILITY OF ESBL-POSITIVE AND ESBL-NEGATIVE ISOLATES OF E. COLI AND KLEBSIELLA PNEUMONIAE TO ANTIMICROBIAL AGENTS(Macedonian Association of Anatomists and Morphologists (MAAM), 2014); ; ; The aim of this investigation was to compare the susceptibility between ESBL-positive and ESBL-negative isolates to different antimicrobial agents. Material and methods: A total of 1207 consecutive non-repeat isolates of E. coli and K. pneumoniae-Kp (E. coli-804, Kp-403) were obtained from different clinical specimens from patients hospitalized at the University Clinics in Skopje over a one year period. Conventional microbiological procedures for the isolation and detection of the strains were used. Susceptibility to beta-lactams and non-beta-lactams was determined by standard disk diffusion method and by automated method using Vitek (for determination of the MIC-minimal inhibitory concentration). A total of 251 isolates were selected (E. coli-126 and K. pneumoniae-125) using tests for phenotypic detection of the ESBL-production. A total of 233 randomly selected strains which were ESBL-negative by phenotypic tests (E.coli-130 and K.pneumoniae103) were used as a control strains for susceptibility testing. Results: Difference of the susceptibility to imipenem between ESBL-positive and ESBL-negative strains of E. coli and K. pneumoniae was not detected. Both groups of strains were 100% susceptible to imipenem. Considering the rest of the tested beta-lactams, as well as non-beta lactams and uroantiseptics, there was a difference in the susceptibility comparing both groups of strains of E. coli and K. pneumoniae. This was due to the fact that ESBL-negative strains were highly susceptible to all tested antimicrobial agents, unlike the ESBL-positive ones (except in cases of cotrimoxazole and nitrofurantoin, where the difference in susceptibility between ESBL-positive and ESBL-negative strains of E. coli was not statistically significant, because both groups of strains were susceptible to those antimicrobial agents).These data are important for recommending adequate treatment of infections with ESBL-producing bacteria. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Chlamydia pneumoniae and helicobacter pylori serology - importance in patients with coronary heart disease(Academy of Medical Sciences of Bosnia and Herzegovina, 2012); ; Chronic infections in CHD are due to one or both of the organisms Chlamydia pneumoniae and Helicobacter pylori. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Osteoarticular Involvement in Childhood Brucellosis(Wolters Kluwer/Lippincott, 2013-08); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Microbiological analysis of wound samples(Medical Faculty, Ss. Cyril and Methodius University in Skopje, 2020); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antimicrobial resistance in Gram-positive bacteria isolated from blood culture(Macedonian Association of Anatomists and Morphologists, 2024-10); ; ; ; Blood cultures are essential in diagnosing the existence of bacteria in the blood and they continue to be the gold standard for the diagnosis of septicemia. From Gram-positive bacteria, Staphylococcus aureus and Enterococcus spp. are the most common bacteria causing sepsis and septic shock. They are the leading causes of hospital-acquired infections globally. Aim of the paper was to identify Staphylococcus aureus and Enterococcus spp. from hemoculture and to determine their susceptibility to antibiotics. Blood samples from hospitalized patients admitted for routine laboratory testing at the Institute of Microbiology from January 2023 to December 2023 were included in the study. Blood cultures bottles were incubated in automated Bact/Alert system. Isolated colonies were identified using automated VITEK 2 Compact system. MSSA and MRSA were isolated in 48.2% and 51.8% respectively. All isolates 100% were resistant to penicillin. Intermediate sensitivity to levofloxacin 92%, 60% and ciprofloxacin 96%, 64% was observed in MSSA and MRSA strains. Resistance to gentamicin of 63% was seen in MSSA isolates. Non-VRE and VRE were isolated in 67% and 33% respectively. Non-VRE strains had higher resistance towards gentamicin 77%, streptomycin 66%. VRE strains were resistant to gentamicin 93%, ampicillin 85%. Our findings give analysis of Gram-positive bacteria isolated from hemoculture and their resistance pattern. The findings give clinicians an insight for the right treatment option. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Multidrug Resistant Bacterial Isolates in Hematopoietic Stem Cell Recipients-Single Center Experience(Elsevier BV, 2019-09); ; ; ; Cadievski, Lazar - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Pneumococcal serotypes and their resistance to penicillin(Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, R Macedonia, 2014); ; ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Susceptibility profile of Candida parapsilosis in critically ill neonates determined with VITEK-2 antifungal susceptibility method(INSTITUTE OF PUBLIC HEALTH OF REPUBLIC OF NORTH MACEDONIA, 2011); ; ; ; Progressive increase in the incidence of serious fungal infections during the last decades has been registered. Although spectrum of fungi causing serious infections continues to expand, Candida species remains responsible for most of them. C.albicans is still the most frequent etiological agent, but shift towards non-albicans Candida species has been noticed. From recently, C.parapsilosis is an emerging pathogen that has increased in significance and prevalence. Premature born neonates and patients in intensive care units are at highest risk for severe infection. Due to emerging resistance in Candida species to antifungal agents, there is a need for determination of susceptibility profile of clinically significant yeasts. The aim of this study was to investigate the susceptibility profile of ten blood cultures’ isolates of C.parapsilosis from critically ill neonates to fluconazole, voriconazole and amphotericin B. Antifungal susceptibility testing of all isolates was performed with the automated VITEK-2 method. All isolates of C.parapsilosis were susceptible to the investigated antifungal agents. The emergence of C.parapsilosis as the leading non-albicans Candida species in the etiology of candidemia in critically ill patients poses a major threat for the future. The VITEK-2 system is the first automated approach to antifungal susceptibility testing and provides optimal susceptibility test standardization. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Seminal Fluid Microbiota and Male Infertility: An Emerging Frontier(Macedonian Association of Anatomists and Morphologists, 2024-10); ; ; ; Infertility is a multifaceted issue affecting millions globally, with male factors contributing to approximately 50% of cases. Traditionally, male infertility has been linked to genetic, hormonal, and environmental factors. However, emerging research highlights that disruptions or imbalances of the microbiota residing in the male reproductive tract, including the prostate, seminal vesicles, and urethra have significant implications for male reproductive health. Male urogenital system, particularly the urethra is home to a wide variety of microorganisms with rather high quantity. The composition of male genital microbiome can vary significantly between individuals and while the core microbiome is consised of a few dominant species a high variability is noted in minor species. It is predominantly composed of bacteria, with species from the genera Lactobacillus, Staphylococcus, Corynebacterium, and Propionibacterium being commonly found. Differentiating between pathogenic bacteria and the usual resident microflora is a difficulty in clinical practice, especially when it comes to male infertility. Furthermore, even though it is often benign, an imbalance in the commensal microbiota might encourage inflammation or foster an atmosphere that is favorable for pathogenic infections. Therefore, it is crucial to comprehend and maintain this equilibrium in order to create successful plans for the treatment and prevention of infertility. Traditional diagnostic approaches on male infertility focus on hormonal levels, sperm analysis, sperm culture and genetic factors, but unlike conventional culture methods which might find it difficult to distinguish between pathogenic and benign microorganisms because of genotypic and phenotypic overlaps and low pathogen presentation, developments in molecular methods for microbiota analysis, offer the possibility of more precise diagnosis and treatment of urogenital infections and imbalances. Modulating the genital microbiome could offer new treatment strategies for male infertility and related conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Surface adherence properties and antimicrobial susceptibility of coagulase negative staphylococci with intrahospital and community origin.(Macedonian Association of Anatomists and Morphologists (MAAM), 2021); ; Introduction: Coagulase-negative staphylococci (CoNS) were long regarded harmless commensals which commonly colonize human skin and mucous membranes. Although CoNS are usually in a benign relationship with the host, changes in the medical practice and also in the underlying host populations resulted in their emerge as opportunistic pathogens with key role primarily in nosocomial but in community-acquired infections as well. Objective: Тo determine the association between surface adherence properties/ antimicrobial susceptibility and the isolates origin in clinically significant CoNS and CoNS from normal skin microbiota of healthcare workers and healthy volunteers. Material and methods: CoNS were identified by sample cultivation on standard microbiological nutrition media, gram staining and biochemical tests. Modification of the microtiter plate assay described by Christensen et al. was used to investigate the formation of biofilm. The antimicrobial susceptibility testing (AST) was performed according to EUCAST standard disc diffusion method guidelines for 13 drugs frequently prescribed as commonly active against CoNS. Results: In present study biofilm production was observed in 9 (90%), 7 (70%) and 2 (20%) CoNS isolates from blood cultures from septic patients, skin swabs from hands of medical professionals and skin samples from hands of healthy volunteers, accordingly. Importantly, our study indicated that 63.3% CoNS isolates were resistant to methicillin. Along with the resistance to methicillin, a multidrug resistance pattern towards benzylpenicillin (63.3%), flucloxacillin (63.3%), ceftriaxone (63.3%) and cefotaxime (63.3%) was noted. The results obtained in the study also showed that, the biofilm forming coagulase negative staphylococci have higher resistance rates to various antibiotics compared to biofilm non- producing bacteria. Conclusion: Highest production of biofilm and multidrug resistance were observed in bacterial isolates obtained from hospital environment. We also observed that the majority of MDR pathogens were biofilm producers and were almost equally present in blood samples from critical patients and skin samples of the hands of healthcare personnel.
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