Surface adherence properties and antimicrobial susceptibility of coagulase negative staphylococci with intrahospital and community origin.
Journal
Acta Morphologica
Date Issued
2021
Author(s)
Abstract
Introduction: Coagulase-negative staphylococci (CoNS) were long regarded harmless
commensals which commonly colonize human skin and mucous membranes. Although CoNS
are usually in a benign relationship with the host, changes in the medical practice and also in
the underlying host populations resulted in their emerge as opportunistic pathogens with key
role primarily in nosocomial but in community-acquired infections as well.
Objective: Тo determine the association between surface adherence properties/
antimicrobial susceptibility and the isolates origin in clinically significant CoNS and CoNS
from normal skin microbiota of healthcare workers and healthy volunteers.
Material and methods: CoNS were identified by sample cultivation on standard
microbiological nutrition media, gram staining and biochemical tests. Modification of the
microtiter plate assay described by Christensen et al. was used to investigate the formation of
biofilm. The antimicrobial susceptibility testing (AST) was performed according to EUCAST
standard disc diffusion method guidelines for 13 drugs frequently prescribed as commonly
active against CoNS.
Results: In present study biofilm production was observed in 9 (90%), 7 (70%) and 2 (20%)
CoNS isolates from blood cultures from septic patients, skin swabs from hands of medical
professionals and skin samples from hands of healthy volunteers, accordingly. Importantly, our
study indicated that 63.3% CoNS isolates were resistant to methicillin. Along with the
resistance to methicillin, a multidrug resistance pattern towards benzylpenicillin (63.3%),
flucloxacillin (63.3%), ceftriaxone (63.3%) and cefotaxime (63.3%) was noted. The results
obtained in the study also showed that, the biofilm forming coagulase negative staphylococci
have higher resistance rates to various antibiotics compared to biofilm non- producing bacteria.
Conclusion: Highest production of biofilm and multidrug resistance were observed in
bacterial isolates obtained from hospital environment. We also observed that the majority of
MDR pathogens were biofilm producers and were almost equally present in blood samples
from critical patients and skin samples of the hands of healthcare personnel.
commensals which commonly colonize human skin and mucous membranes. Although CoNS
are usually in a benign relationship with the host, changes in the medical practice and also in
the underlying host populations resulted in their emerge as opportunistic pathogens with key
role primarily in nosocomial but in community-acquired infections as well.
Objective: Тo determine the association between surface adherence properties/
antimicrobial susceptibility and the isolates origin in clinically significant CoNS and CoNS
from normal skin microbiota of healthcare workers and healthy volunteers.
Material and methods: CoNS were identified by sample cultivation on standard
microbiological nutrition media, gram staining and biochemical tests. Modification of the
microtiter plate assay described by Christensen et al. was used to investigate the formation of
biofilm. The antimicrobial susceptibility testing (AST) was performed according to EUCAST
standard disc diffusion method guidelines for 13 drugs frequently prescribed as commonly
active against CoNS.
Results: In present study biofilm production was observed in 9 (90%), 7 (70%) and 2 (20%)
CoNS isolates from blood cultures from septic patients, skin swabs from hands of medical
professionals and skin samples from hands of healthy volunteers, accordingly. Importantly, our
study indicated that 63.3% CoNS isolates were resistant to methicillin. Along with the
resistance to methicillin, a multidrug resistance pattern towards benzylpenicillin (63.3%),
flucloxacillin (63.3%), ceftriaxone (63.3%) and cefotaxime (63.3%) was noted. The results
obtained in the study also showed that, the biofilm forming coagulase negative staphylococci
have higher resistance rates to various antibiotics compared to biofilm non- producing bacteria.
Conclusion: Highest production of biofilm and multidrug resistance were observed in
bacterial isolates obtained from hospital environment. We also observed that the majority of
MDR pathogens were biofilm producers and were almost equally present in blood samples
from critical patients and skin samples of the hands of healthcare personnel.
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