Jakjovski, Zlatko
Preferred name
Jakjovski, Zlatko
Official Name
Jakjovski, Zlatko
Main Affiliation
Email
zlatko.jakjovski@medf.ukim.edu.mk
42 results
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Item type:Publication, Reliable and durable Golgi staining of brain tissue from human autopsies and experimental animals(Elsevier BV, 2014-06-15); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Comparative study of two dna extraction methods in different tissues and conditions of degradation(Elsevier BV, 2015-12); ; ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Air gun injury with deadly aftermath – Case report(Elsevier BV, 2013-01); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, INFORMED CONSENT IN GENETIC REASEARCH(Macedonian Association of Anatomists and Morphologists, 2023); ; ; ; Recognizing the ethical, legal, and social implications (ELSI) of genetic testing becomes crucial for physicians in the face of complex medical issues, as they are increasingly expected to counsel their patients regarding the medical, psychological, and social responses arising from genetic information. Genetic medicine, with its extreme complexity and the potential repercussions on an individual's life, raises important questions in the ethical, deontological, and legal realms of medicine, playing a primary role in personalized medicine. The aim of this paper is to underscore the significance of informed consent and to provide insights into the ethical procedures associated with genetic testing. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, CCNG1 and FDXR gene expression levels after radiation therapy in breast cancer patients(Univerzitet "Sv.Kiril i Metodij" - Medicinski fakultet Skopje, 2021-12-30); ; ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Determining Specific Thyroid Transcripts in Peripheral Blood: A Single Center Study Experience(Walter de Gruyter GmbH, 2018-12); ; ; ; Thyroid carcinoma (TC) comprises a spectrum of different tumors with a wide range of biological behavior and prognosis. The techniques based on the latest trends in molecular biology may have application in diagnosis of metastatic TC. The aim of this study was to apply and analyze mRNA expression in peripheral blood of thyrotropin receptor [thyroid stimulating hormone receptor (TSHR-mRNA)] gene and thyroglobulin (Tg-mRNA) gene using 2-ΔΔCt method in differentiated TC patients and healthy individuals. Fifty-seven subjects were included in the study, consisting of 40 patients with TC and 17 healthy volunteers as a control group. Total RNA was isolated from peripheral blood and used for two-step reverse transcriptase-polymerase chain reaction (PCR). Real-time PCR was performed with appropriate primers. Relative quantification using the 2-ΔΔCt method was applied. Thyroid carcinoma patients with metastatic disease or loco-regional relapse expressed TSHR-mRNA by a 8.57-fold higher level than healthy controls. Thyroid carcinoma patients with biochemical relapse expressed TSHR-mRNA by a 14.17-fold higher level than healthy controls, while expression of Tg-mRNA was 6.6-fold higher in TC patients with metastatic disease and loco-regional relapse than healthy controls and 8.34-fold higher level compared with TC patients with excellent response to treatment. Our preliminary study showed that the TSHR gene expression might have more useful application as a biomarker compared to detection of Tg gene expression. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, TIME-DEPENDENT EXPRESSION OF IL-1β mRNA - A PRELIMINARY STUDY FOR POSSIBLE WOUND AGE MARKER(Macedonian Association of Anatomists and Morphologists, 2023); ; ; ; Siamkouri, RosicaAccurate estimation of wound age is a very important task in forensic medicine field, because it can helps in reconstruction of crime scene. IL-1β is proinflamatory cytokine and plays a major role in wound healing process. The aim of the study is to develop a method for the reliable estimation of dermal injury age. In this study the expression of IL-1β mRNA was evaluated by real-time PCR at skin human wounds with different post-injuried period. We collected 27 human skin wound samples from forensic autopsy cases at our Institute. In our study were include samples from lacerations, incised wounds and stab wounds, with a known time of injury and death. Wound samples were divided into five groups: control group (n=5); first group consisted of cases with immediate death (n=7); second group consisted of cases with survival from 1 hour to 6 hours (n=5); the third group comprised cases with survival from 6 hours to 72 hours (n=3); the fourth group comprised cases with survival from 72 hours to 168 hours (n=7). In this study we detected increased expression levels of IL-1 β mRNA in the second group with survival time from 1 to 6 hours. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Correlation of mRNA-TSHR expression with clinical parameters in thyroid carcinoma patients(Nature Publishing Group, 2019-10-10); ; ; ; Jovanovic, H - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Thyroid Stimulating Hormone Receptor Transcripts in Correlation with Clinical Parameters in Thyroid Carcinoma Patients(Scientific Foundation SPIROSKI, 2020-09-23); ; ; ; <jats:p>BACKGROUND: Differentiated thyroid carcinomas (DTC) preserve expression of thyroid stimulating hormone receptor (TSHR). AIM: The aim of our study was to evaluate the expression of mRNA-TSHR in peripheral blood of DTC patients, then to correlate the expression with clinical features: Serum thyroglobulin (sTg) value, initial staging and findings from the whole body scan (WBS), neck ultrasound (US), and total received dose of radioiodine therapy. MATERIALS AND METHODS: Forty patients were divided into three groups according to the treatment response: Patients with incomplete structural response (TCs), incomplete biochemical response (TCb), and excellent responders (TCr). Total RNA was isolated from peripheral blood and used for two-step reverse transcriptase-PCR with appropriate primers. Relative quantification using the ΔCt and 2–ΔΔCt and method was applied. sTg levels were evaluated with chemiluminescent assay. The statistical analysis was performed with Spearman Rank Order Correlation. RESULTS: We found that TCs patients expressed mRNA-TSHR by a 5.37-fold higher level than TCr patients, TCb patients expressed TSHR by an 8.88-fold higher level than TCr patients. A significant negative correlation was detected between sTg and ΔCt (CtTSHR - CtGAPDH) value (R = −0.475; p < 0.05), and between WBS/US findings and ΔCt (R= −0.321; p < 0.05). CONCLUSION: Our data revealed higher expression of mRNA-TSHR in peripheral blood of TCs and TCb compared to TCr patients and analysis revealed a significant correlation between mRNA-TSHR and sTg and US/WBS findings. Further studies with larger number of subjects and absolute quantification are needed for understanding the real meaning of mRNA-TSHR as a biomarker in DTC.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Correlation of mRNA-TSHR and mRNA-Tg with radioiodine whole body scan and ultrasound findings in thyroid carcinoma patients(Springer Science and Business Media LLC, 2019-10); ; ; ;