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Title: Comparative Studies of the Uptake and Internalization Pathways of Different Lipid Nano-Systems Intended for Brain Delivery
Other Titles: Cellular Uptake Mechanisms and Internalization Pathways of Lipid Nano-Systems
Authors: Mihailova Ljubica
Shalabalija Dushko
Zimmer, Andreas
Geškovski, Nikola 
Makreski, Petre 
Petrushevska, Marija 
Simonoska crcarevska, Maja 
Glavas Dodov, Marija 
Issue Date: 3-Aug-2023
Publisher: MDPI AG
Journal: Pharmaceutics
Abstract: Lipid nano-systems were prepared and characterized in a series of well-established in vitro tests that could assess their interactions with the hCMEC/D3 and SH-SY5Y cell lines as a model for the blood-brain barrier and neuronal function, accordingly. The prepared formulations of nanoliposomes and nanostructured lipid carriers were characterized by z-average diameters of ~120 nm and ~105 nm, respectively, following a unimodal particle size distribution (PDI < 0.3) and negative Z-potential (-24.30 mV to -31.20 mV). Stability studies implied that the nano-systems were stable in a physiologically relevant medium as well as human plasma, except nanoliposomes containing poloxamer on their surface, where there was an increase in particle size of ~26%. The presence of stealth polymer tends to decrease the amount of adsorbed proteins onto a particle's surface, according to protein adsorption studies. Both formulations of nanoliposomes were characterized by a low cytotoxicity, while their cell viability was reduced when incubated with the highest concentration (100 μg/mL) of nanostructured lipid formulations, which could have been associated with the consumption of cellular energy, thus resulting in a reduction in metabolic active cells. The uptake of all the nano-systems in the hCMEC/D3 and SH-SY5Y cell lines was successful, most likely following ATP-dependent internalization, as well as transport via passive diffusion.
ISSN: 1999-4923
DOI: 10.3390/pharmaceutics15082082
Appears in Collections:Faculty of Pharmacy: Journal Articles

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