Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/25766
Title: CHANGES OF SPLEEN IN WISTAR RATS EXPOSED TO THERAPEUTIC DOSES OF DEXAMETHASONE AND MEDROXYPROGESTERONE ACETATE EVALUATED BY STEREOLOGICAL PARAMETERS
Authors: Mitevska, Elida 
Kostadinova Petrova, Irena 
Kostovska, Nevena 
Keywords: spleen
Wistar rats
stereological analysis
dexamethasone and medroxyprogesterone acetate
Issue Date: 2015
Publisher: Macedonian Academy of Sciences and Arts
Journal: Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki) 
Abstract: The aim of our investigation was to evaluate the immunosuppressive effect of medroxyprogesterone acetate (MPA) determining the volume densities of the structural components of the spleen. The volume densities of the same structural components of spleen were determined after administration of dexamethasone too, in order to see whether the morphological changes induced by MPA are in the same line with the changes caused by dexamethasone. 60 female Wistar rats were divided into 5 groups. The control group of rats was administered phy siological solution. The remaining, 4 experimental groups were administered: dexamethasone at a therapeutic daily dose of 0.6 mg/kg bw and maximal therapeutic dose of 3 mg/kg bw, and MPA at a therapeutic dose of 30 mg/kg bw and maximal therapeutic dose of 150 mg/kg bw. The drugs were applied intramuscularly for 7 days. Spleen paraffin sections were stained according to the methods: hematoxylin-eosin, Masson and Elastica van-Gieson. Stereological measurements were performed by using the Weibl’s multipurpose test system (M-42). The histological analyses of the structural components of the spleen in rats treated with dexametha sone and MPA have shown reduction of the white pulp and the marginal zone and an apparent decrease of the cellular density of the lymphocyte component of the pulp. The stereological analysis of the spleen showed significant decrease of the splenic pulp volume density and significant increase of the connective tissue volume density. Reducing the presence of splenic pulp was mainly due to the decrease in the volume density of all structural components of the white pulp. Changes were obser ved in all drug treated groups of rats. Our results have shown that the MPA provoked changes suggested atrophy of the spleen lymphoid tissue. Although the atrophic changes of the spleen were significant after the application of both dexamethasone and MPA, the white pulp was significantly more sensitive substrate for dexametha sone than for the MPA
URI: http://hdl.handle.net/20.500.12188/25766
ISSN: 1857-9345
DOI: 10.1515/prilozi-2015-0074
Appears in Collections:Faculty of Medicine: Journal Articles

Show full item record

Page view(s)

30
checked on Jun 22, 2024

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.