Preliminary results of introducing the method multiparameter flow cytometry in patients with acute leukemia in Republic of Macedonia
Journal
Macedonian Journal of Medical Sciences
Date Issued
2008-12-05
Author(s)
Hadzi-Pecova, Liljana
DOI
10.3889/MJMS.1857-5773.2008.0021
Abstract
Abstract
Background. In this paper we present the initial results of introducing the method
of multiparameter flow cytometry (MPF) in patients with acute leukemia in the
Republic of Macedonia.
Aim. The aim of our study is to improve the diagnosis and management of acute
leukemia, to establish the correct lineage assignment of the blast cells and to select
effective treatment strategy for each single acute leukemia patient.
Material and methods. A total of 44 adult (>15 years) patients (from initially 45
tested) with acute leukemia who were consecutively admitted at the Clinic of
Hematology-Skopje from January through June 2008, were enrolled in this study.
The MPF was introduced for the first time in the Republic of Macedonia and was
performed at the Institute for Immunobiology and Human Genetics, Faculty of
Medicine-Skopje.
Results. Our results showed that morphology and cytochemistry established
lineage in 39 of patients, but not in 5 cases that presented as acute leukemia, of
which 4 were assigned as myeloid and in one nonhematopoietic malignancy was
indicated. Furthermore immunophenotyping change the lineage assigned based
on morphology and cytochemistry in one case from lymphoid to myeloid. Results
from our study showed that routine immunophenotyping improved the diagnosis
in 6 (13.3%) cases. The exact lineage assignment of the blasts cells guides to
implementation of specific molecular analyses in some subtypes of acute leukemia
and their further definition, which is essential for more appropriate single patient
therapeutic decisions.
Conclusion. Our data support routine implementation of MPF in the diagnostic
evaluation of acute leukemia.
Background. In this paper we present the initial results of introducing the method
of multiparameter flow cytometry (MPF) in patients with acute leukemia in the
Republic of Macedonia.
Aim. The aim of our study is to improve the diagnosis and management of acute
leukemia, to establish the correct lineage assignment of the blast cells and to select
effective treatment strategy for each single acute leukemia patient.
Material and methods. A total of 44 adult (>15 years) patients (from initially 45
tested) with acute leukemia who were consecutively admitted at the Clinic of
Hematology-Skopje from January through June 2008, were enrolled in this study.
The MPF was introduced for the first time in the Republic of Macedonia and was
performed at the Institute for Immunobiology and Human Genetics, Faculty of
Medicine-Skopje.
Results. Our results showed that morphology and cytochemistry established
lineage in 39 of patients, but not in 5 cases that presented as acute leukemia, of
which 4 were assigned as myeloid and in one nonhematopoietic malignancy was
indicated. Furthermore immunophenotyping change the lineage assigned based
on morphology and cytochemistry in one case from lymphoid to myeloid. Results
from our study showed that routine immunophenotyping improved the diagnosis
in 6 (13.3%) cases. The exact lineage assignment of the blasts cells guides to
implementation of specific molecular analyses in some subtypes of acute leukemia
and their further definition, which is essential for more appropriate single patient
therapeutic decisions.
Conclusion. Our data support routine implementation of MPF in the diagnostic
evaluation of acute leukemia.
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