Molecular detection of virus herpes simplex type 1 (HSV-1), virus herpes simplex type 2 (HSV-2), Cytomegalovirus (HCMV) and Epstein-barr virus (EBV) in supra-gingival dental plaque in patients with periodontal disease.
Journal
KNOWLEDGE – International Journal, Vol. 28.2, p. 415-422
Date Issued
2018-12
Author(s)
Ivanovska-Stojanoska, Marija
Zendeli-Bedjeti, Lindita
Todorovska, Sashka
Abstract
Introduction: Chronic periodontitis is as an inflammatory disease of the supporting tissue of the teeth caused by periopatogens microorganisams. New concept about etiology of periodontal disease suggests that herpes viruses may play an important role in the pathogenesis of periodontal disease. The aim of this study was to analyze the presence of herpes simplex virus type 1and 2 (HSV-1, HSV-2) cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), in supra-gingival dental plaque.
Materials and Methods: The study comprised a total of 89 patients who were divided in two groups: patients who were diagnosed moderate stage of periodontal disease and patients who were diagnosed with advanced stage of periodontal disease. Clinical examinations included determination of plaque index, gingival index, sulcus bleeding index, probing depth, and clinical attachment level. Supra-gingival dental plaque samples were taken with sterile coton ball scrub on tooth enamel. Molecular detection of HSV1, HSV2 , EBV and CMV was analyzed using polymerase chain reaction (PCR).
Results: Molecular analysis of HSV-1 ,HSV-2, EBV and CMV in supra-gingival dental plaque in patients with chronic periodontal disease (a total of 89) showed the presence of EBV in 12,40% of patients , HSV-1 was found in 11,20% of patients, in 7,90% of patients was detected HSV-2, CMV was established in 9,00% of patients. In 3,40% patients in supra-gingival dental plaque was detected combination of viruses HSV1 and CMV, in 3,40% of patient combination of HSV1 and EBV ; and in 2,20% of patient combination ofHSV2,EBV was observed; in 2,20% of patients was found combination of 3 viruses HSV1; HSV2; EBV and in 49,50% patients we found negative finding. Patients with moderate stage of periodontal disease have a 0,78 times(OR = 0,78 / 0,33-1,84/)no
significantly lower risk of probability of finding viruses in the supra-gingival plaque compared to patients who had advanced stage of periodontal disease. In the shown cross-culture of the presence of viruses in supra-gingival plaque in patients with moderate and advanced stage of periodontal disease for Fisher, s Exact Test = 5,19 and p>0.05 (p = 0.809 / Monte Carlo sig. (0.799-0.819)there is no significant difference.
Conclusion: We can associate the presence of herpes simplex viruses in supra-gingival dental plaque with periodontal disease. Our findings suggest that there is no significant difference in presence of viruses in supra gingival plaque in patients with moderate and severe stage of periodontal disease.
Materials and Methods: The study comprised a total of 89 patients who were divided in two groups: patients who were diagnosed moderate stage of periodontal disease and patients who were diagnosed with advanced stage of periodontal disease. Clinical examinations included determination of plaque index, gingival index, sulcus bleeding index, probing depth, and clinical attachment level. Supra-gingival dental plaque samples were taken with sterile coton ball scrub on tooth enamel. Molecular detection of HSV1, HSV2 , EBV and CMV was analyzed using polymerase chain reaction (PCR).
Results: Molecular analysis of HSV-1 ,HSV-2, EBV and CMV in supra-gingival dental plaque in patients with chronic periodontal disease (a total of 89) showed the presence of EBV in 12,40% of patients , HSV-1 was found in 11,20% of patients, in 7,90% of patients was detected HSV-2, CMV was established in 9,00% of patients. In 3,40% patients in supra-gingival dental plaque was detected combination of viruses HSV1 and CMV, in 3,40% of patient combination of HSV1 and EBV ; and in 2,20% of patient combination ofHSV2,EBV was observed; in 2,20% of patients was found combination of 3 viruses HSV1; HSV2; EBV and in 49,50% patients we found negative finding. Patients with moderate stage of periodontal disease have a 0,78 times(OR = 0,78 / 0,33-1,84/)no
significantly lower risk of probability of finding viruses in the supra-gingival plaque compared to patients who had advanced stage of periodontal disease. In the shown cross-culture of the presence of viruses in supra-gingival plaque in patients with moderate and advanced stage of periodontal disease for Fisher, s Exact Test = 5,19 and p>0.05 (p = 0.809 / Monte Carlo sig. (0.799-0.819)there is no significant difference.
Conclusion: We can associate the presence of herpes simplex viruses in supra-gingival dental plaque with periodontal disease. Our findings suggest that there is no significant difference in presence of viruses in supra gingival plaque in patients with moderate and severe stage of periodontal disease.
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