Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV

Journal
Microorganisms
Date Issued
2021-06-06
Author(s)
Vidanović, Dejan
Tešović, Bojana
Šekler, Milanko
Debeljak, Zoran
Vasković, Nikola
Matović, Kazimir
Koltsov, Andrey
Petrović, Tamaš
De Leeuw, Ilse
Haegeman, Andy
DOI
10.3390/microorganisms9061234
Abstract
<jats:p>Lumpy skin disease (LSD) is an important animal disease with significant health and economic impacts. It is considered a notifiable disease by the OIE. Attenuated strains of LSDV have been successfully used as vaccines (LAV) but can also produce mild or systemic reactions. Vaccination campaigns using LAVs are therefore only viable if accompanying DIVA assays are available. Two DIVA qPCR assays able to distinguish Neethling-based LAVs and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic performance comparable to other published DIVA assays. This confirmed their potential as reliable tools to confirm infection in animals during vaccination campaigns based on Neethling vaccine strains.</jats:p>
Subjects

validation

lumpy skin disease

PCR

DIVA

vaccination

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