Implementation of Novel Mode for Evaluation of MYCN Amplification that can Predict Outcome in Patients with Neuroblastoma
Journal
CLINICAL ONCOLOGY AND RESEARCH
Date Issued
2020
Author(s)
Ilieva Gordana
Conevska Biljana
DOI
http://dx.doi.org/10.31487/j.COR.2020.11.04
Abstract
Background: Neuroblastoma tumorigenesis is a cascading process where several cytogenetic findings can
be detected MYCN oncogene is a potent transcription factor that controls main cell functions. Several
genetic methods can be applied in order to detect quantity of amplified MYCN oncogene. The purpose of
this study is to improve the technique of determining the amplification of the MYCN oncogene in each
evaluated tumor cell.
Results: Standard G banded karyotype and fluorescence in-situ hybridization (FISH) on interphase nuclei
using N-MYC amplification probe was performed in five patients with different clinical presentation of
neuroblastoma. Both bone marrow and tumor tissue were analysed in four and in one patient only tumor
tissue. Follow up study was performed in order to obtain additional prognostic information. Additional
grading system was implemented to obtain MYCN amplification status. Significant amount of amplified
units was detected in two patients with adverse outcome, which was not the case in other three patients who
had minor or none amplification of MYCN. Furthermore, there were no cells with significant MYCN
amplification in more than 30% of the cell surface in patient three and four that represented a good
prognostic factor for their survival.
Conclusion: Our study confirmed that patients with both chromosomal changes and significant MYCN
amplification are characterized with aggressive clinical course. Accuracy in quantifying the amount of
MYCN amplification is crucial in planning the therapeutic approach. FISH is proved to be rapid, sensitive,
and reliable method for detection of MYCN oncogene amplification in routinely processed samples
be detected MYCN oncogene is a potent transcription factor that controls main cell functions. Several
genetic methods can be applied in order to detect quantity of amplified MYCN oncogene. The purpose of
this study is to improve the technique of determining the amplification of the MYCN oncogene in each
evaluated tumor cell.
Results: Standard G banded karyotype and fluorescence in-situ hybridization (FISH) on interphase nuclei
using N-MYC amplification probe was performed in five patients with different clinical presentation of
neuroblastoma. Both bone marrow and tumor tissue were analysed in four and in one patient only tumor
tissue. Follow up study was performed in order to obtain additional prognostic information. Additional
grading system was implemented to obtain MYCN amplification status. Significant amount of amplified
units was detected in two patients with adverse outcome, which was not the case in other three patients who
had minor or none amplification of MYCN. Furthermore, there were no cells with significant MYCN
amplification in more than 30% of the cell surface in patient three and four that represented a good
prognostic factor for their survival.
Conclusion: Our study confirmed that patients with both chromosomal changes and significant MYCN
amplification are characterized with aggressive clinical course. Accuracy in quantifying the amount of
MYCN amplification is crucial in planning the therapeutic approach. FISH is proved to be rapid, sensitive,
and reliable method for detection of MYCN oncogene amplification in routinely processed samples
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