Please use this identifier to cite or link to this item:
http://hdl.handle.net/20.500.12188/11559
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Giorda, R | en_US |
dc.contributor.author | Lampasona, V | en_US |
dc.contributor.author | Kocova, M | en_US |
dc.contributor.author | Trucco, M | en_US |
dc.date.accessioned | 2021-03-25T11:21:10Z | - |
dc.date.available | 2021-03-25T11:21:10Z | - |
dc.date.issued | 1993-11 | - |
dc.identifier.issn | 0736-6205 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12188/11559 | - |
dc.description.abstract | We describe a new nonradioisotopic method for HLA class II molecular typing performed in 96-well plates of the same size as those used in enzyme-linked immunosorbent assays (ELISA) and radioimmunoassays (RIA). Biotinylated sequence-specific oligonucleotide (SSO) probes are bound to avidin-coated plates. Digoxigenin-labeled PCR-amplified DNA samples are hybridized, washed and detected with a peroxidase conjugated antibody assay. The method was tested by performing a partial HLA DQA1 and DQB1 typing on 69 randomly selected blood samples. The results are completely concordant with a traditional SSO-PCR typing performed on the same samples. This procedure is simple, fast and could be adapted for performance in semi-automated or automated fashion using equipment already available for ELISA and RIA assays. | en_US |
dc.language.iso | en | en_US |
dc.publisher | INFORMA HEALTHCARE | en_US |
dc.relation.ispartof | BioTechniques | en_US |
dc.title | Non-radioisotopic typing of human leukocyte antigen class II genes on microplates | en_US |
dc.type | Article | en_US |
dc.identifier.volume | 15 | - |
dc.identifier.issue | 5 | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Faculty of Medicine | - |
Appears in Collections: | Faculty of Medicine: Journal Articles |
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