PULSED-FIELD GEL ELECTROPHORESIS USED FOR TYPING OF EXTENDED-SPECTRUM-β-LACTAMASES- PRODUCING ESCHERICHIA COLI ISOLATED FROM INFANT ҆ S RESPIRATORY AND DIGESTIVE SYSTEM
Journal
Macedonian Veterinary Review
Date Issued
2018-10-15
Author(s)
Popova, Gorica
Felix, Benjamin
Stojanovska - Dimzovska, Biljana
DOI
10.2478/macvetrev-2018-0016
Abstract
Escherichia coli infections are becoming increasingly difficult to treat because of emerging antimicrobial resistance, mostly
to expanded-spectrum cephalosporins, due to the production of extended-spectrum β-lactamases (ESBLs).Despite extensive
studies of ESBL- producing E.coli in adult patients, there is a lack of information about the epidemiology and spread of ESBL
organisms in pediatric population. The aim of this study was to examine the gastrointestinal tract as an endogenous reservoir
for the respiratory tract colonization with ESBL- E. coli in children, hospitalized because of the severity of the respiratory
illness. The study group consists of 40 children with ESBL-producing E. coli strains isolated from the sputum and from the
rectal samples. A control group of 15 E. coli isolated from rectal swabs of healthy children were included in the analysis. The
comparison of the strains was done by using antimicrobial susceptibility patterns of the stains, and pulsed field gel electrophoresis
was performed for molecular typing, using XbaI digestion. 90% of the compared pairs of strains in the study group were with
identical antimicrobial susceptibility patterns and indistinguishable in 79.2% by the obtained PFGE – profiles.33.3% (5/15) of
confirmed E. coli strains from the control group were found to be ESBL – producers. Resulting band profiles of all isolates
demonstrated presence of 12 pulsotypes, with 100% similarity within the pulsotypes. Although, some isolates obtained from
different patients were genetically indistinguishable, these strains were not hospital acquired, as none of the patients satisfied the
criteria for hospital acquired pneumonia, and there was a lack of an obvious transmission chain. All ESBL –E. coli isolated from
sputum in clinical cases were obtained from patients under the age of one. According to the resistance profile of the compared
pairs and the PFGE comparison of all isolates, it can be concluded that the gastrointestinal tract is the main reservoir of ESBL-E.
coli. Small age in infants is a risk factor for translocation of bacteria, enabling the colonization of the respiratory tract.
to expanded-spectrum cephalosporins, due to the production of extended-spectrum β-lactamases (ESBLs).Despite extensive
studies of ESBL- producing E.coli in adult patients, there is a lack of information about the epidemiology and spread of ESBL
organisms in pediatric population. The aim of this study was to examine the gastrointestinal tract as an endogenous reservoir
for the respiratory tract colonization with ESBL- E. coli in children, hospitalized because of the severity of the respiratory
illness. The study group consists of 40 children with ESBL-producing E. coli strains isolated from the sputum and from the
rectal samples. A control group of 15 E. coli isolated from rectal swabs of healthy children were included in the analysis. The
comparison of the strains was done by using antimicrobial susceptibility patterns of the stains, and pulsed field gel electrophoresis
was performed for molecular typing, using XbaI digestion. 90% of the compared pairs of strains in the study group were with
identical antimicrobial susceptibility patterns and indistinguishable in 79.2% by the obtained PFGE – profiles.33.3% (5/15) of
confirmed E. coli strains from the control group were found to be ESBL – producers. Resulting band profiles of all isolates
demonstrated presence of 12 pulsotypes, with 100% similarity within the pulsotypes. Although, some isolates obtained from
different patients were genetically indistinguishable, these strains were not hospital acquired, as none of the patients satisfied the
criteria for hospital acquired pneumonia, and there was a lack of an obvious transmission chain. All ESBL –E. coli isolated from
sputum in clinical cases were obtained from patients under the age of one. According to the resistance profile of the compared
pairs and the PFGE comparison of all isolates, it can be concluded that the gastrointestinal tract is the main reservoir of ESBL-E.
coli. Small age in infants is a risk factor for translocation of bacteria, enabling the colonization of the respiratory tract.
File(s)![Thumbnail Image]()
Loading...
Name
macvetrev-2018-0016.pdf
Size
1.8 MB
Format
Adobe PDF
Checksum
(MD5):50caa7e088c77332e73c42c4aa21078a