UTILIZING MALDI-TOF MS FOR CONFIRMATION OF BACTERIAL ISOLATES IN ROUTINE MICROBIOLOGY ANALYSIS OF FOOD
Date Issued
2024-09-26
Author(s)
Prodanov Mirko, Manovska Ratkova Marija, Shikoska Ivana, Cvetkovikj Iskra
Abstract
Food safety is of great importance for global public health. Microbiological contamination and foodborne
infections pose a major part of this risk. Consequently, detecting, monitoring, and characterizing pathogens
present substantial challenges in quality control environments. The European and the Macedonian regulation
have stick rules of the ISO methods that should be used for the testing of food. This are classic microbiological
methods witch include culture techniques and biochemical tests, are often recognized for being highly timeconsuming
and labor-intensive. The regulation (EU and Macedonian) allows alternative methods only if they are
validated according to ISO 16140-6. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) is a
mass spectrometry technique that identifies microorganisms by analyzing their unique protein fingerprints. It
works by ionizing proteins in a sample, measuring their mass-to-charge ratio, and comparing the resulting
spectra to a database for rapid and accurate identification of bacterial isolates. This method is highly efficient and
much faster than traditional culture and biochemical techniques. This method is validated according to ISO
16140-6 for the Salmonella spp. Listeria spp. Listeria monocytogenes and Campylobacter spp. Upon short
verification process MALDI-TOF is successfully incorporated in the confirmation of the accredited methods for
food borne pathogens in our laboratory. The utilization of this technology in the regular laboratory practice has
resulted in shorter reporting time when confirmation is needed, and decrees of the need to reagents for
confirmation.
infections pose a major part of this risk. Consequently, detecting, monitoring, and characterizing pathogens
present substantial challenges in quality control environments. The European and the Macedonian regulation
have stick rules of the ISO methods that should be used for the testing of food. This are classic microbiological
methods witch include culture techniques and biochemical tests, are often recognized for being highly timeconsuming
and labor-intensive. The regulation (EU and Macedonian) allows alternative methods only if they are
validated according to ISO 16140-6. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) is a
mass spectrometry technique that identifies microorganisms by analyzing their unique protein fingerprints. It
works by ionizing proteins in a sample, measuring their mass-to-charge ratio, and comparing the resulting
spectra to a database for rapid and accurate identification of bacterial isolates. This method is highly efficient and
much faster than traditional culture and biochemical techniques. This method is validated according to ISO
16140-6 for the Salmonella spp. Listeria spp. Listeria monocytogenes and Campylobacter spp. Upon short
verification process MALDI-TOF is successfully incorporated in the confirmation of the accredited methods for
food borne pathogens in our laboratory. The utilization of this technology in the regular laboratory practice has
resulted in shorter reporting time when confirmation is needed, and decrees of the need to reagents for
confirmation.
File(s)![Thumbnail Image]()
Loading...
Name
2024_UTILIZING MALDI-TOF MS FOR CONFIRMATION OF BACTERIAL ISOLATES IN ROUTINE MICROBIOLOGY ANALYSIS OF FOOD.pdf
Size
565.56 KB
Format
Adobe PDF
Checksum
(MD5):4384f7f74e923d66df17aee1311b5f53