APPLICATION OF THE SINGLE CELL GEL ELECTROPHORESIS FOR DETECTION OF OXIDATIVE DNA DAMAGE DUE TO LONG TERM EXPOSURE TO SUPRAPHYSIOLOGIC DOSES OF L-THYROXINE
Journal
Acta Morphologica
Date Issued
2018
Author(s)
Atanasovska M
Bundovski K
Todorovska l
Abstract
Since reactive oxygen species (ROS) induced by thyroid hormones may cause lipid and
protein oxidation as well as DNA damage, the main aim of this article is to investigate the
genotoxic effect (in vivo) of the supraphysiologic doses of L-thyroxinein apolipoprotein E
deficient homozygous (ApoE-/-) mice. For this purpose, the method of single-cell gel
electrophoresis that allows the detection of DNA damage in individual cell caused by various
endogenous and exogenous agents was used. The Student t-test has shown no significant
difference between the examined parameters of the thyroxine-treated group compared with
untreated control group of animals. The positive control (treated with solution of H2O2) has
significantly higher values of the examined parameters compared with the treated and
untreated control group of animals. The results confirm that chronic exposure to high doses
of thyroxine do not cause damage to the DNA molecule that could be detected by the method
of single-cell gel electrophoresis.
protein oxidation as well as DNA damage, the main aim of this article is to investigate the
genotoxic effect (in vivo) of the supraphysiologic doses of L-thyroxinein apolipoprotein E
deficient homozygous (ApoE-/-) mice. For this purpose, the method of single-cell gel
electrophoresis that allows the detection of DNA damage in individual cell caused by various
endogenous and exogenous agents was used. The Student t-test has shown no significant
difference between the examined parameters of the thyroxine-treated group compared with
untreated control group of animals. The positive control (treated with solution of H2O2) has
significantly higher values of the examined parameters compared with the treated and
untreated control group of animals. The results confirm that chronic exposure to high doses
of thyroxine do not cause damage to the DNA molecule that could be detected by the method
of single-cell gel electrophoresis.