Antibacterial effects of conventional glass ionomer cement
Journal
Bratisl Med J
Date Issued
2016
Author(s)
Nicholson, JW
Abstract
OBJECTIVES: The antibacterial activity of conventional glass ionomer cement against three different microorganism strains alone and following incorporation of 1, 2 and 3 % Benzalkonium Chloride and Cetylpyridinium
Chloride was evaluated.
METHODS: Agar diffusion method was used to determine the inhibitory effect of the conventional glass ionomer
cement ChemFlex on Streptococcus mutans, Lactobacillus casei and Actinomyces viscosus. Bacterial strains
were inoculated into BHIB, and incubated in an anaerobic atmosphere (37 °C). From the bacteria grown in the
liquid medium, the density of the inoculum was set to be equivalent to McFarland 2 standard. In Shaedler agar,
350 μL of the bacterial suspension were equally spread. Specimens (4 mm × 6 mm) were prepared from the
cement without and with addition of 1, 2 and 3 % Benzalkonium Chloride and Cetylpyridinium Chloride. The
inhibition zones were determined after 48 hours, after 2, 7 and 21 days of incubation.
RESULTS: The combination ChemFlex + Benzalkonium Chloride has the best effect on the three analysed bacteria.
The Benzalkonium Chloride antibacterial compound has a stronger antibacterial effect than Cetylpyridinium Chloride.
CONCLUSIONS: Glass ionomer cements can potentially be used as a medium for slow release of active antimicrobial components, and they have the potential to improve clinical outcomes of the cements
Chloride was evaluated.
METHODS: Agar diffusion method was used to determine the inhibitory effect of the conventional glass ionomer
cement ChemFlex on Streptococcus mutans, Lactobacillus casei and Actinomyces viscosus. Bacterial strains
were inoculated into BHIB, and incubated in an anaerobic atmosphere (37 °C). From the bacteria grown in the
liquid medium, the density of the inoculum was set to be equivalent to McFarland 2 standard. In Shaedler agar,
350 μL of the bacterial suspension were equally spread. Specimens (4 mm × 6 mm) were prepared from the
cement without and with addition of 1, 2 and 3 % Benzalkonium Chloride and Cetylpyridinium Chloride. The
inhibition zones were determined after 48 hours, after 2, 7 and 21 days of incubation.
RESULTS: The combination ChemFlex + Benzalkonium Chloride has the best effect on the three analysed bacteria.
The Benzalkonium Chloride antibacterial compound has a stronger antibacterial effect than Cetylpyridinium Chloride.
CONCLUSIONS: Glass ionomer cements can potentially be used as a medium for slow release of active antimicrobial components, and they have the potential to improve clinical outcomes of the cements
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