IMPACT OF MAMMARY GLAND INFECTIVE STATUS ON THE CONCENTRATION OF LACTOFERRIN IN COW’S MILK
Journal
JHED
Date Issued
2021
Author(s)
Trajkovska, Biljana
Kochoski, Ljupche
Petrovski, Kiro
Dimitrovska, Gordana
Abstract
Lactoferrin (LF) is glycoprotein, which is normally
excreted in cow’s milk, but as a result of inflammatory
processes in the mammary gland, its concentration
is being increased. The aim of this research was to
determinate whether the LF concentration in milk is
influenced by increased number of milk somatic cell
count.
In this research we used milk from (n = 345) Holstein-
Friesian cows in Pelagonia region, or more precisely
milk from healthy (n = 112) and infected quarters (n
= 233). A quarter was classified as healthy when no
visible signs of mastitis were detected or the California
Mastitis Test (CMT) was negative. A quarter was
classified as subclinical when no visible signs of mastitis
were detected but CMT was positive. A quarter was
classified as clinical mastitis when there were visible
signs of mastitis (cow, udder or milk appearance). The
milk samples were analysed instrumentally for physico
- chemical properties (Lactoscope C4+), somatic
cell count (SCC) by Bentley Somacount CC 150, total
number of bacteria (CFU) (by Bentley Bactocount IBC),
and by ELISA for concentrations of lactoferrin. We used
SPSS 15.0 for analysis of gained data.
Significant differences were detected in concentrations
of lactoferrin in healthy quarters (915.94 μg/mL)
and in quarters with sub-clinical (2,095.87 μg/mL)
and clinical mastitis (3,528.99 μg/mL) (p < 0.001). A
correlation between lactoferrin and lactose (r = -0.21,
p < 0.01), lactoferrin and CFU (r = 0.28, p < 0.01) as well
as between lactoferrin and somatic cells (r = 0.32, p <
0.01) was observed. These results initiate that lactoferrin could be used
as complementary test to somatic cell count, and
potential to be a primary diagnostic test for subclinical
and clinical mastitis in dairy cows. Furthermore,
it could be used as natural antibiotic in treatment
of mastitis in dairy cows, in order to decrease the
presence of antibiotic residuals in milk, thereby to
improve hygiene of raw milk.
excreted in cow’s milk, but as a result of inflammatory
processes in the mammary gland, its concentration
is being increased. The aim of this research was to
determinate whether the LF concentration in milk is
influenced by increased number of milk somatic cell
count.
In this research we used milk from (n = 345) Holstein-
Friesian cows in Pelagonia region, or more precisely
milk from healthy (n = 112) and infected quarters (n
= 233). A quarter was classified as healthy when no
visible signs of mastitis were detected or the California
Mastitis Test (CMT) was negative. A quarter was
classified as subclinical when no visible signs of mastitis
were detected but CMT was positive. A quarter was
classified as clinical mastitis when there were visible
signs of mastitis (cow, udder or milk appearance). The
milk samples were analysed instrumentally for physico
- chemical properties (Lactoscope C4+), somatic
cell count (SCC) by Bentley Somacount CC 150, total
number of bacteria (CFU) (by Bentley Bactocount IBC),
and by ELISA for concentrations of lactoferrin. We used
SPSS 15.0 for analysis of gained data.
Significant differences were detected in concentrations
of lactoferrin in healthy quarters (915.94 μg/mL)
and in quarters with sub-clinical (2,095.87 μg/mL)
and clinical mastitis (3,528.99 μg/mL) (p < 0.001). A
correlation between lactoferrin and lactose (r = -0.21,
p < 0.01), lactoferrin and CFU (r = 0.28, p < 0.01) as well
as between lactoferrin and somatic cells (r = 0.32, p <
0.01) was observed. These results initiate that lactoferrin could be used
as complementary test to somatic cell count, and
potential to be a primary diagnostic test for subclinical
and clinical mastitis in dairy cows. Furthermore,
it could be used as natural antibiotic in treatment
of mastitis in dairy cows, in order to decrease the
presence of antibiotic residuals in milk, thereby to
improve hygiene of raw milk.
Subjects
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