ASSOCIATION OF METHYLENETETRAHYDROFOLATEREDUCTASE GENE POLIMORPHISMS C677T ON THE HOMOCYSTEINELEVELS IN CORONARY ARTHERYDIASEASE
Journal
Balkan Journal of Clinical Laboratory
Date Issued
2015-10
Author(s)
Marija Krstevska
Nikola Sekulovski
Anthony Pashovski
Abstract
Background: The aims of this paper
were to determine the concentration of
the total homocysteine(tHcy), to find
whether the increased level of tHcy is associated with mutation of the C677T
methylenetetrahydro- folatereductase
gene (MTHFR). Also, we wanted to show
whether the determination of the
polymorphisms of this gene is
particularly involved in the establishing
of the diagnosis of coronary artery
disease (CAD), and whether this genetic
analysis is the choice for this most
common disease.
Subjects and methods: The study
included 84 subjects divided into two
main groups: 43 healthy subjects as
control group and 41 patients with CAD.
The concentration of tHcy was
determined by a cyclic enzymatic
method, and the mutation of the MTHFR
C677T gene was examined by a
polymerase chain reaction. Results: The concentration of tHcy
plasma in the patients with CAD was
significantly higher (18,72 ± 5,31 μmol /
L) compared to the control group (11,11
± 3,23 μmol / L) (p <0.001). The
statistical analysis with multiple
regression showed that at the genotypes
CT and TT of the MTHFR (C677T), the
tHcy level is not significantly higher than
the CC genotype of MTHFR (C677T) nor
in the control group nor in the patients
with CAD.
Conclusion: The analysis of the results
showed that the polymorphism of the
MTHFR (C677T) gene is not associated,
in most cases, with the mild to moderate
hyperhomocysteinemia.
were to determine the concentration of
the total homocysteine(tHcy), to find
whether the increased level of tHcy is associated with mutation of the C677T
methylenetetrahydro- folatereductase
gene (MTHFR). Also, we wanted to show
whether the determination of the
polymorphisms of this gene is
particularly involved in the establishing
of the diagnosis of coronary artery
disease (CAD), and whether this genetic
analysis is the choice for this most
common disease.
Subjects and methods: The study
included 84 subjects divided into two
main groups: 43 healthy subjects as
control group and 41 patients with CAD.
The concentration of tHcy was
determined by a cyclic enzymatic
method, and the mutation of the MTHFR
C677T gene was examined by a
polymerase chain reaction. Results: The concentration of tHcy
plasma in the patients with CAD was
significantly higher (18,72 ± 5,31 μmol /
L) compared to the control group (11,11
± 3,23 μmol / L) (p <0.001). The
statistical analysis with multiple
regression showed that at the genotypes
CT and TT of the MTHFR (C677T), the
tHcy level is not significantly higher than
the CC genotype of MTHFR (C677T) nor
in the control group nor in the patients
with CAD.
Conclusion: The analysis of the results
showed that the polymorphism of the
MTHFR (C677T) gene is not associated,
in most cases, with the mild to moderate
hyperhomocysteinemia.
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