VISUALISATION OF MICROGLIA WITH THE USE OF IMMUNOHISTOCHEMICAL DOUBLE STAINING METHOD FOR CD-68 AND Iba-1 OF CEREBRAL TISSUE SAMPLES IN CASES OF BRAIN CONTUSIONS
Journal
Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki)
Date Issued
2015-12
Author(s)
Chakar, Ljupco
Rosoklija, Gorazd
Abstract
In the recent years it has been confirmed that the main component of the immune response in an
injury of the nerve cell comes from microglia and macrophages. The main challenge in the field of
microglia research is to detect the different stages of cellular activation by visualization of the cell
morphology. The existing visualization techniques are based on surface molecules expression in
resting and activated microglia cells. For visualization of the microglial cells and their functional
state we used double labeling method for cd-68 and iba1 in brain contusions with different survival
time. Microglia are stained brown with Iba-1, whereas microglia impregnated with black, grainy
color, represents activated microglia stained with CD 68. We had significantly positive results, and
we were able to observe changes in the morphology of the microglia that correlated with the survival time. Using double labeling with Iba-1 and cd68 we were able to determine their physiological state based on the morphology and immunoreactivity
injury of the nerve cell comes from microglia and macrophages. The main challenge in the field of
microglia research is to detect the different stages of cellular activation by visualization of the cell
morphology. The existing visualization techniques are based on surface molecules expression in
resting and activated microglia cells. For visualization of the microglial cells and their functional
state we used double labeling method for cd-68 and iba1 in brain contusions with different survival
time. Microglia are stained brown with Iba-1, whereas microglia impregnated with black, grainy
color, represents activated microglia stained with CD 68. We had significantly positive results, and
we were able to observe changes in the morphology of the microglia that correlated with the survival time. Using double labeling with Iba-1 and cd68 we were able to determine their physiological state based on the morphology and immunoreactivity
File(s)![Thumbnail Image]()
Loading...
Name
Visualisation_of_Microglia_with_the_use_of_Immunoh.pdf
Size
1.06 MB
Format
Adobe PDF
Checksum
(MD5):0b604b0224ce6a58e32acae2d17a87df