ISOLATION AND CHARACTERIZATION OF POTENTIAL ANTIBIOTIC-PRODUCING BACILLUS AND ACTINOMYCETES FROM UNDISTURBED AND CONTAMINATED SOILS FROM NORTH MACEDONIA
Journal
BIOLOGY, BIOTECHNOLOGY, BIOMEDICINE XVII International Summer School Conference Proceedings
Date Issued
2022-07-11
Author(s)
Sofija Kostandinovska
Natalija ATANASOVA-PANCHEVSKA
Dzoko Kungulovski
Abstract
The Actinomycetes and Bacillus are diversely distributed microorganisms
in nature. The geographical diversity of North Macedonia is suitable
for adaptation of various species of these bacteria. Also, the distribution of the
actinomycetes and Bacillus is dependent upon the altitude, nature and contamination
of the soil. The goal of this study was to determine how common culturable
antibiotic-producing Actinomycetes and Bacillus bacteria are in undisturbed and
contaminated sites.
A total of 5 soil samples were collected from different regions of North
Macedonia and were processed. Different isolates were characterized by observing
colony characteristics and microscopic characteristics. Screening of the antimicrobial
property was based upon perpendicular line streaking method.
From the identification tool used, 120 were found to be Bacillus spp., 112
were Actinomycetes. Out of total samples, 28 isolates of Actinomycetes and 26 isolates
of Bacillus spp. were tested effective against the tested bacteria by primary
screening using the cross streak method. Among the primarily screened, the Actinomycetes
and Bacillus spp. from undisturbed sites showed better antimicrobial
properties towards the tested bacteria that the ones from the contaminated sites.
In summary, antibiotic-producing microorganisms were found in most soils
even by using a relatively limited screening assay.
in nature. The geographical diversity of North Macedonia is suitable
for adaptation of various species of these bacteria. Also, the distribution of the
actinomycetes and Bacillus is dependent upon the altitude, nature and contamination
of the soil. The goal of this study was to determine how common culturable
antibiotic-producing Actinomycetes and Bacillus bacteria are in undisturbed and
contaminated sites.
A total of 5 soil samples were collected from different regions of North
Macedonia and were processed. Different isolates were characterized by observing
colony characteristics and microscopic characteristics. Screening of the antimicrobial
property was based upon perpendicular line streaking method.
From the identification tool used, 120 were found to be Bacillus spp., 112
were Actinomycetes. Out of total samples, 28 isolates of Actinomycetes and 26 isolates
of Bacillus spp. were tested effective against the tested bacteria by primary
screening using the cross streak method. Among the primarily screened, the Actinomycetes
and Bacillus spp. from undisturbed sites showed better antimicrobial
properties towards the tested bacteria that the ones from the contaminated sites.
In summary, antibiotic-producing microorganisms were found in most soils
even by using a relatively limited screening assay.
Subjects
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