Occurrence of aflatoxins in peanuts and peanut products determined by liquid chromatography with fluorescence detection
Journal
Zbornik Matice srpske za prirodne nauke
Date Issued
2013
Author(s)
Stojanovska-Dimzoska, Biljana
Hajrulai-Musliu, Zehra
Dimitrieska-Stojkovic, Elizabeta
Uzunov, Risto
DOI
10.2298/zmspn1324027s
Abstract
<jats:p>Liquid chromatography with fluorescence detection using immunoaffinity
column clean-up was a method described for determination of aflatoxins (AFB1,
AFB2, AFG1 and AFG2) in peanuts and peanut based products. The validation of
the procedure was performed. Good coefficient of correlation was found for
all aflatoxins in the range of 0.9993-0.9999. Limit of detection (LOD) and
limit of quantification (LOQ) ranged from 0.003-0.005 mg/kg and 0.009-0.023
mg/kg, respectively, which was acceptable. The mean recovery for total
aflatoxins was 88.21%. The method also showed acceptable precision values in
the range of 0.171-2.626% at proposed concentration levels for all four
aflatoxins. RSDR values (within laboratory reproducibility) calculated from
the results showed good correlation between two analysts for all aflatoxins
and they ranged from 4.93-11.87%. The developed method was applied for the
determination of aflatoxins in 27 samples of peanuts and peanut based
products. The results showed that 21 peanut samples (77.7%) were below LOD of
the method. Three samples had positive results over the MRL. There was one
extreme value recorded for the total aflatoxins in peanut (289.2 mg/kg) and
two peanut based products, peanut snack and peanut, with total content of
aflatoxins being 16.3 mg/kg and 8.0 mg/kg, respectively. The obtained results
demonstrated that the procedure was suitable for the de?termination of
aflatoxins in peanuts and peanut based products and it could be implemented
for the routine analysis.</jats:p>
column clean-up was a method described for determination of aflatoxins (AFB1,
AFB2, AFG1 and AFG2) in peanuts and peanut based products. The validation of
the procedure was performed. Good coefficient of correlation was found for
all aflatoxins in the range of 0.9993-0.9999. Limit of detection (LOD) and
limit of quantification (LOQ) ranged from 0.003-0.005 mg/kg and 0.009-0.023
mg/kg, respectively, which was acceptable. The mean recovery for total
aflatoxins was 88.21%. The method also showed acceptable precision values in
the range of 0.171-2.626% at proposed concentration levels for all four
aflatoxins. RSDR values (within laboratory reproducibility) calculated from
the results showed good correlation between two analysts for all aflatoxins
and they ranged from 4.93-11.87%. The developed method was applied for the
determination of aflatoxins in 27 samples of peanuts and peanut based
products. The results showed that 21 peanut samples (77.7%) were below LOD of
the method. Three samples had positive results over the MRL. There was one
extreme value recorded for the total aflatoxins in peanut (289.2 mg/kg) and
two peanut based products, peanut snack and peanut, with total content of
aflatoxins being 16.3 mg/kg and 8.0 mg/kg, respectively. The obtained results
demonstrated that the procedure was suitable for the de?termination of
aflatoxins in peanuts and peanut based products and it could be implemented
for the routine analysis.</jats:p>