Comparison of ELISA and chemiluminescence immunoassay methods for quantification of human Placental growth factor in serum
Journal
Molecular and Experimental Biology in Medicine
Date Issued
2020-06-01
Author(s)
Bashkim Ismaili
Gorjan Milanovski
Boban Dobrevski
DOI
10.33602/mebm.3.1.10
Abstract
Placental growth factor (PlGF) is crucial during placental development in early
pregnancy. Several studies in pregnancies with complications such as
preeclampsia or small for gestational age neonates find that PlGF levels are
significantly lower in the first trimester, which implies that the
concentration of PlGF could be used as an early screening biomarker for these
conditions. This study aimed to compare the performance of chemiluminescence
immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) for the
quantification of human PlGF in serum. This is a comparative study on 88
pregnant women in the first trimester subjected to measurement of PlGF in serum
using two commercially available kits: Human PlGF Quantikine HS ELISA (R&D
Systems) and PlGF CLIA (Snibe). The overall coefficient of correlation between
the tests was 0.93. When the cut-off value of 40 pg/mL was applied, it dropped
significantly to 0.50 towards the lower values, while remaining an excellent
0.91 in the group with higher concentrations of PlGF. While R&D Systems’s
ELISA seems to have better sensitivity, it is not very convenient to use for a
small number of samples. Snibe’s CLIA automated method is user-friendly, fast
and powerful. Both tests show excellent performance when indicating risk-free
pregnancies.
pregnancy. Several studies in pregnancies with complications such as
preeclampsia or small for gestational age neonates find that PlGF levels are
significantly lower in the first trimester, which implies that the
concentration of PlGF could be used as an early screening biomarker for these
conditions. This study aimed to compare the performance of chemiluminescence
immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) for the
quantification of human PlGF in serum. This is a comparative study on 88
pregnant women in the first trimester subjected to measurement of PlGF in serum
using two commercially available kits: Human PlGF Quantikine HS ELISA (R&D
Systems) and PlGF CLIA (Snibe). The overall coefficient of correlation between
the tests was 0.93. When the cut-off value of 40 pg/mL was applied, it dropped
significantly to 0.50 towards the lower values, while remaining an excellent
0.91 in the group with higher concentrations of PlGF. While R&D Systems’s
ELISA seems to have better sensitivity, it is not very convenient to use for a
small number of samples. Snibe’s CLIA automated method is user-friendly, fast
and powerful. Both tests show excellent performance when indicating risk-free
pregnancies.