TOTAL PHOSPHORUS QUANTIFICATION IN ANIMAL FEED, MILK, MEAT AND THEIR PRODUCTS USING THE ISO 13730:1996 METHOD
Date Issued
2024-09-22
Author(s)
Tome Nestorovski
Stefan, Jovanov
Abstract
Phosphorus is a macro-element naturally present in animal feed, meat, milk and their products. Due to
its beneficial properties, it is also used as an additive in these food groups. Monitoring total phosphorus
concentrations in all food and feed products, whether it occurs naturally or is added during processing,
is crucial for health safety. A high and unregulated intake of phosphorus can lead to side effects such
as cardiovascular and kidney diseases as well as other complications in both humans and animals. The
aim of this study was to integrate the phosphorus quantification method for animal feed described in
ISO 6491:1998, into the previously optimized spectroscopy conditions for total phosphorus
quantification in both meat and milk food matrices as a continuation of the research. The proficiency
test results, with assigned value of 5870 mg / kg yielded a Z – score of - 0.3. Following this, the method
was verified by determining the standard deviation (SD) for each sample series, the repeatability (r),
and the expanded uncertainty (U). The results have confirmed that the conditions of the ISO 13730:1996
method are suitable for accurately quantifying total phosphorus content in all animal feed, meat and
milk matrices. This unification contributes to reducing the cost and time required for performing the
analysis, and ensures excellent precision in quantifying total phosphorus content, thus expanding the
scope of application.
its beneficial properties, it is also used as an additive in these food groups. Monitoring total phosphorus
concentrations in all food and feed products, whether it occurs naturally or is added during processing,
is crucial for health safety. A high and unregulated intake of phosphorus can lead to side effects such
as cardiovascular and kidney diseases as well as other complications in both humans and animals. The
aim of this study was to integrate the phosphorus quantification method for animal feed described in
ISO 6491:1998, into the previously optimized spectroscopy conditions for total phosphorus
quantification in both meat and milk food matrices as a continuation of the research. The proficiency
test results, with assigned value of 5870 mg / kg yielded a Z – score of - 0.3. Following this, the method
was verified by determining the standard deviation (SD) for each sample series, the repeatability (r),
and the expanded uncertainty (U). The results have confirmed that the conditions of the ISO 13730:1996
method are suitable for accurately quantifying total phosphorus content in all animal feed, meat and
milk matrices. This unification contributes to reducing the cost and time required for performing the
analysis, and ensures excellent precision in quantifying total phosphorus content, thus expanding the
scope of application.
Subjects
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