IMMUNOHISTOCHEMICAL STUDY OF SODIUM-DEPENDENT GLUCOSE CO-TRANSPORTERS IN OSTRICHES KIDNEYS
Date Issued
2020-11
Author(s)
Hussar, Piret
Dūrītis, Ilmārs
Popovska-Percinic, Florina
Järveots, Tõnu
DOI
10.15159/jas.20.23
Abstract
Out of the two families of glucose transporters identified, the sodium-dependent glucose co-transporters contribute to renal glucose reabsorption. Due to the lack of knowledges of the localization of SGLTs in bird's kidneys, the present study aimed to immunolocalize Na+-glucose co-transporters SGLT1 and SGLT2 in ostrich's kidneys. In the study kidney material derived from five 14 days old female ostriches. Material 0.5–1.0 cm in diameter was fixed in 10% formalin, dehydrated, embedded into paraffin; thereafter slices 7 μm in thickness were cut and deparaffi-nized, followed by immunohistochemical staining with polyclonal primary antibodies Rabbit anti-SGLT1 and Rabbit anti-SGLT2 (Abcam, UK) according to the manufacturers' guidelines (IHC kit, Abcam, UK). Our study revealed the immunohistochemical localization of SGLT1 and SGLT2 in the proximal tubules of the renal cortex. The immunohisto-chemical locations of sodium-dependent glucose transporters resembled those in mammals.
Subjects