STUDY ON THE EFFECTIVENESS OF A MULTI-TOXIN IMMUNOAFFINITYCLEAN-UP FOR RELIABLE COST-EFFECTIVE HPLC-FLD ANALYSISOF MYCOTOXINS IN CORN-BASED FOODS
Journal
Macedonian Journal of Chemistry and Chemical Engineering
Date Issued
2022-06-20
Author(s)
Biljana Stojanovska-Dimzoska
Zehra Hajrulai-Musliu
Risto Uzunov
Aleksandra Angeleska
Katrina Blagoevska
Radmila Crceva-Nikolovska
Gordana Ilievska
Elizabeta Dimitrieska-Stojkovikj
DOI
10.20450/mjcce.2022.2422
Abstract
A different, reliable, and cost-effective strategy for the analysis of aflatoxins, ochratoxin A, and zearalenone in corn-based foods was proposed, including one multi-toxin immunoaffinity column (IAC) sample preparation and three different high-performance liquid chromatography fluorescence detection methods. The analytical procedures were tested and verified, keeping in mind their occurrence at trace levels in corn-based foods. With the validation of the proposed multi-toxin IAC methodology and comparison of the performance characteristics with methods using a single-toxin IAC, we confirmed the reliability of the multi-toxin IAC procedure versus the single-toxin IAC. The methods were validated by revealing satisfactory performance characteristics; for example, the obtained values of limit of detection were significantly lower than the maximum limits for all mycotoxins of concern. In addition, the recovery values were between 70.9 % and 106.1 % for all mycotoxins of interest, with precision values lower than 10.5 %.
Subjects