GENOTYPES OF ESBL PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE IN RELATION TO RESISTANCE TO ANTIMICROBIAL DRUGS
Journal
Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki)
Date Issued
2014
Author(s)
Gordana Jankoska
Abstract
The aim of the study was to evaluate the association of drug resistance with β-lactamase gene types
in ESBL positive E. coli and Klebsiella pneumoniae-Kp.
Material and methods: A total of 251 ESBL-positive E. coli and Kp isolates obtained from urine,
tracheal aspirate, wound swab and blood from patients hospitalised at the University Clinics in
Skopje were detected using the ESBL set and automated Vitek 2 system. Vitek was also used for
susceptibility testing (determination of MIC of 17 antimicrobial agents). Multiplex PCR was used to
identify genes for different types of ESBLs in a 100 randomly selected, ESBL positive strains.
Results: More of the 87 ESBL typeable isolates (61%) harbour two or more bla genes and the frequency of antibiotic resistance was high in these isolates, compared to those with a single gene. Isolates with ≥ 3 genes were highly resistant to beta-lactams and non-beta lactams used. The degree of
resistance to 3rd generation cephalosporins was also high in these isolates (MIC ≥ 64). More of the
ESBL-positive isolates showed higher resistance to cefotaxime than to ceftazidime.
Conclusion: Identification of the genes is necessary for the surveillance of their transmission in hospitals.
Surveillance of antibiotic resistance patterns are crucial to overcome the problems associated with ESBLs.
in ESBL positive E. coli and Klebsiella pneumoniae-Kp.
Material and methods: A total of 251 ESBL-positive E. coli and Kp isolates obtained from urine,
tracheal aspirate, wound swab and blood from patients hospitalised at the University Clinics in
Skopje were detected using the ESBL set and automated Vitek 2 system. Vitek was also used for
susceptibility testing (determination of MIC of 17 antimicrobial agents). Multiplex PCR was used to
identify genes for different types of ESBLs in a 100 randomly selected, ESBL positive strains.
Results: More of the 87 ESBL typeable isolates (61%) harbour two or more bla genes and the frequency of antibiotic resistance was high in these isolates, compared to those with a single gene. Isolates with ≥ 3 genes were highly resistant to beta-lactams and non-beta lactams used. The degree of
resistance to 3rd generation cephalosporins was also high in these isolates (MIC ≥ 64). More of the
ESBL-positive isolates showed higher resistance to cefotaxime than to ceftazidime.
Conclusion: Identification of the genes is necessary for the surveillance of their transmission in hospitals.
Surveillance of antibiotic resistance patterns are crucial to overcome the problems associated with ESBLs.
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