MOLECULAR DETECTION OF EHRLICHIA CANIS IN THE PET-DOG POPULATION IN R. N. MACEDONIA
Journal
Acta Veterinaria-Beograd
Date Issued
2021
Author(s)
Atanaskova Petrov, Elena
Popova, Zagorka
Djadjovski, Igor
DOI
10.2478/acve-2021-0020
Abstract
Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused
by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick,
Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic,
or Mediterranean climates (Central and South America, Eastern and Western Asia,
Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska
et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog
population. Up to date, the presence of E. canis, using molecular diagnostic methods,
has not been investigated in Macedonia. Therefore, this study aimed to confirm the
presence of E. canis, in the pet-dog population on the territory of the city of Skopje,
North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR).
Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms
of CME and positive serology result for the presence of antibodies against E.canis, were
collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present
work reports the first molecular detection of E. canis in pet dogs on the territory of the
city of Skopje, Macedonia.
by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick,
Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic,
or Mediterranean climates (Central and South America, Eastern and Western Asia,
Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska
et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog
population. Up to date, the presence of E. canis, using molecular diagnostic methods,
has not been investigated in Macedonia. Therefore, this study aimed to confirm the
presence of E. canis, in the pet-dog population on the territory of the city of Skopje,
North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR).
Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms
of CME and positive serology result for the presence of antibodies against E.canis, were
collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present
work reports the first molecular detection of E. canis in pet dogs on the territory of the
city of Skopje, Macedonia.
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