Now showing 1 - 10 of 22
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    Platelet-rich plasma as a promising bioscaffold for enhancing peripheral nerve regeneration: An experimental study in a rat sciatic nerve model
    (Journal of Biological Methods, 2025)
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    Aleksovski, Boris
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    Tusheva, Sofija
    Despite advancements in surgical treatments, impairments persist after peripheral nerve injuries, prompting a shift in research toward the microenvironment of injured axons. Platelet-rich plasma (PRP), rich in growth factors and derived from autologous blood, emerges as a potential candidate to accelerate nerve healing.
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    Validation of screening method for determination of methyltestosterone in fish
    (Mac Vet Rev, 2013-03-15)
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    Stojanovska-dimzoska biljana
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    Todorovic Aleksandra
    Anabolic androgenic steroids are synthetic derivatives of testosterone, which is the primary male sex hormone. These anabolic agents are used to increase the weight gain, to improve the food efficiency, storing proteins and to decrease fatness. However, depending on the use of anabolic agent in animal feed, anabolic residues that may occur in meat and meat products present risks to human health. The aim of this study was the validation of screening ELISA method for determination of methyltestoterone anabolic steroid in fish. The validation process was carried out according to Commission Decision 2002/657/EC criteria. The detection limit for methyltestosterone was 140.95 ng/kg and the detection capability was 564.43 ng/kg. The overall recoveries and the coefficients of variation (CV) were in the range of 82.4%-97.4% and 1.5%-6.9%, respectively, a working range between 50 to 4050 ng/kg, and the regression equation of the final inhibition curve was: y= -0,1741x + 1,5082, R2 = 0.9927. Because of the good recovery and precision, and satisfactory detection capability, this method is applicable in official control laboratories as a rapid screening method for determination of methyltestosterone in fish.
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    A New Lc-Ms/Ms Method for Multiple Residues/Contaminants In Bovine Meat
    (Research Square, 2020-07-10)
    Hajrulai-Musliu, Zehra
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    Uzunov, Risto
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    Jovanov, Stefan
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    <jats:title>Abstract</jats:title> <jats:p>A multi-class and multi-residue/contaminant method for the determination of veterinary drug and pesticide residues and mycotoxins in bovine meat has been developed and validated. The veterinary drug residues/contaminants included antimicrobials, anabolic hormones, lactones, β-agonists, mycotoxins, and pesticides. Isotopic labeled internal standards were included to compensate residual matrix effects. The calibrators used in the method demonstrated linearity with the coefficient of correlation (R2) > 0.98. The decision limit (CCα) values were in the range from 0.067 to 2103.84 μg/kg, while the range for detection capability (CCβ) was from 0.083 to 2482.13 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were in the range from 0.059 to 291.36 μg/kg, and 0.081 to 328.13 μg/kg, respectively. The recovery of analytes ranged from 61.28 % to 116.20 %. The intra-day coefficient of variation (CV) was from 0.97 to 25.93 % and the inter-day CV was 2.30-34.04 %. The method has been used for the determination of 49 residues/contaminants in bovine meat.</jats:p>
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    Multi-class/residue method for determination of veterinary drug residues, mycotoxins and pesticide in urine using LC-MS/MS technique
    (Springer Science and Business Media LLC, 2023-09-14)
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    Jovanov, Stefan
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    Musliu, Dea
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    Background Veterinary drugs are widely used in animals to prevent diseases and are a complex set of drugs with very different chemical properties. Multiclass and multi-residue methods for simultaneous detection of residues from veterinary drugs and contaminants in urine are very rare or non-existent. Therefore, the aim of this study was to develop and validate a sensitive and reliable quantitative LC-MS/MS method for simultaneous determination of a wide range of veterinary drug and pesticide residues and mycotoxins in bovine urine. This involved 42 veterinary drug residues (4 thyreostats, 6 anabolic hormones, 2 lactones, 10 beta agonists, 15 antibiotics, 5 sulphonamides), 28 pesticides and 2 mycotoxins. Stable isotopically labelled internal standards were used to facilitate effective quantification of the analytes. Analysis was performed in both positive and negative ionization modes with multiple reaction monitoring transitions over a period of 12 min. Results The parameters validated included linearity, limit of detection (LOD), limit of quantification (LOQ), detection capability (CCβ), decision limit (CCα), stability, accuracy and precision. The process followed guidelines of the regulation 2021/808/EC. The calibration curves were linear with coefficient of correlation (R2) from 0.991 to 0.999. The LODs were from 0.01 to 2.71 µg/L, while the LOQs were from 0.05 to 7.52 µg/L. The CCα and CCβ were in range 0.05–12.11 µg/L and 0.08–15.16 µg/L. In addition, the average recoveries of the spiked urine samples were from 71.0 to 117.0% and coefficient of variation (CV) < 21.38% (intraday and interday). Conclusion A new isotopic LC-MS/MS method has been developed, validated and applied for identification and quantification of 72 residues of veterinary drugs and pesticides and other contaminants such as mycotoxins in bovine urine. The most appropriated sample preparation procedures involved sodium acetate buffer, enzymatic hydrolysis using β-glucuronidase and cleanup solid phase extraction with OASIS SPE cartridges. The parameters were satisfactorily validated fulfilling requirements under Regulation 2021/808/EC. Consequently, the method could be used in routine analysis of bovine urine samples for simultaneous detection of veterinary drug and pesticide residues as well as contaminants such as mycotoxins.
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    Item type:Publication,
    Determination of Aflatoxins B1, B2, G1 and G2 in animal feeds with fluorimetric method
    (Српско ветеринарно друштво, Факултет за ветеринарна медицина, Србија, 2011-09-17)
    Zehra Hajrulai-Musliu
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    Elizabeta Dimitrieska-Stojkovikj
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    Biljana Stojanovska-Dimzoska
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    Pavle Sekulovki
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    Item type:Publication,
    Determination of trenbolone in cattle meat with ELISA method
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, 2012-10-04)
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    Zehra Hajrulai-Musliu
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    Elizabeta Dimitrieska-Stojkovikj
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    Biljana Stojanovska-Dimzoska
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    Pavle Sekulovki
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    Item type:Publication,
    A new LC-MS/MS method for multiple residues/contaminants in bovine meat
    (Springer Science and Business Media LLC, 2021-12-08)
    Hajrulai-Musliu, Zehra
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    Uzunov, Risto
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    Jovanov, Stefan
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    A multi-class and multi-residue/contaminant method for the determination of veterinary drug and pesticide residues and mycotoxins in bovine meat has been developed and validated. The veterinary drug residues/contaminants included antimicrobials, anabolic hormones, lactones, β-agonists, mycotoxins, and pesticides. Isotopic labeled internal standards were included to compensate residual matrix effects. The calibrators used in the method demonstrated linearity with the R2 > 0.98. The decision limit (CCα) values were in the range from 0.067 to 2103.84 μg/kg, while the range for detection capability (CCβ) was from 0.083 to 2482.13 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were in the range from 0.059 to 291.36 μg/kg, and 0.081 to 328.13 μg/kg, respectively. The recovery of analytes ranged from 61.28% to 116.20%. The intra-day coefficient of variation (CV) was from 0.97 to 25.93% and the inter-day CV was 2.30-34.04%. The method has been used for the determination of 49 residues/contaminants in bovine meat. Application of the method in routine analysis in bovine samples, revealed in limited samples the presences of enrofloxacin, oxytetracycline and sulfadiazine at the concentration of 35.22 µg/kg, 27.35 µg/kg, and 36.20 µg/kg, respectively.
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    Therapeutic effects of goat milk
    (Vasile Goldis University Press Arad, 2011-09-23)
    Zehra Hajrulai-Musliu
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    Mila Arapceska
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    Biljana Stojanovska-Dimzoska
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    Elizabeta Dimitrieska-Stojkovikj
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    Item type:Publication,
    Determination of zeranol residues levels in bovine urine with ELISA method
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, 2012-09-04)
    Zehra Hajrulai-Musliu
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    Elizabeta Dimitrieska-Stojkovikj
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    Biljana Stojanovska-Dimzoska
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    Pavle Sekulovki
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    Item type:Publication,
    DETERMINATION OF COMONLY USED ARTIFICIAL COLORS IN MEAT AND MEAT PRODUCTS USING HPLC-DAD METHOD
    (Faculty of Veterinary Medicine - Skopje, 2024-09-22)
    Stefan, Jovanov
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    Tome Nestorovski
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    The color of the meat is the most crucial visual quality parameter from the consumer's perspective. Red color additives are used to improve the appearance of meat and meat products on the shelf. Although natural colors like red wine or beet juice are used, there are artificial colors which can withstand the temperature and pH conditions of processing. According the Food additive Regulation in North Macedonia, Carmoisine (Azorubine), Allura Red, Ponceau 4R and Carminic Acid are permitted for use. The allowed limits of these colors in meat and meat products range from 25 mg/kg to 200 mg/kg depending on the additive and whether the meat product is heat treated or not. The aim of this study is to develop a HPLC-DAD method to determine these additives in meat and meat products. Due to the different chemical properties of carminic acid compared to the others, two different HPLC-DAD methods with different sample preparation were developed. Both used RP C18 column and gradient elution with methanol and phosphate buffer, but with different pH levels. Method validation was performed according to ICH Harmonised Guideline Q2(R2) (2022). The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ), and found to be linear, with a correlation coefficient (R2) ranging from 0.995 to 0.999. The LOD and LOQ values calculated from the calibration curve ranged from 0.10 to 0.23 mg/kg and 1.00 to 1.12 mg/kg respectively. The accuracy of the method was evaluated based on the recovery percentages at 3 spiked levels (1, 5 and 10 mg/kg), which were found to be within an acceptable range of 93.82 to 109.30%. The repeatability of the method was assesed at the same three spiking levels over two days, with relative standard deviation (RSDR) values ranging from 2.01 % to 4.97 %. These performance parameters indicate that the developed and validated method is suitable for the quantitative determination of Carmoisine (Azorubine), Allura Red, Ponceau 4R and Carminic Acid in meat and meat products. Random meat products analysis using this method revealed that 15 out of 55 contained these additives but none exceeded the legislative limits