Dimkov, Aleksandar

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Incorporation of antimicrobial agents can be used to enhance the antibacterial effect of endodontic sealers
(Elsevier, 2013-03)
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J.W. Nicholson
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Aim. The antibacterial activity of five endodontic sealers against three different microorganism strains alone and following incorporation of 2% benzalkonium chloride (BC) and 2% cetylpyridinium chloride (CPC) was evaluated. Methodology. The agar diffusion method was used to determine the inhibitory effect of the following endodontic sealers: RoekoSeal, Endomethasone N, N2, Apexit Plus and AH plus, on Streptococcus mutans – ATCC 25175, Lactobacillus casei – ATCC 4646 and Actinomyces viscosus – ATCC 19246. Bacterial strains were inoculated into BHIB, and incubated in an anaerobic atmosphere (37 ◦C). From the bacteria grown in the liquid medium, the density of the inoculum was setto be equivalentto McFarland 2 standard. In Shaedler agar, 350L ofthe bacterial suspension were equally spread. Specimens (4mm× 6mm) were prepared from each material without and with addition of 2% BC or 2% CPC. The inhibition zones were determined after 2 days, after 7 days and after 21 days of incubation. Results. The largest inhibition zones were shown at zero time in all cases, with progressively less inhibition at 7 and 21 days. Endomethasone N and N2 showed the most intense antimicrobial activity, while RoekoSeal showed the least antimicrobial effect. The most susceptible microorganism was A. viscosus. Greater antimicrobial effects were found following incorporation of BC or CPC, and generally, BC gave greater inhibition zones than CPC. Conclusions. Adding either BC or CPC has the potential to improve clinical outcomes with endodontic sealers, as these substances enhance the short-term antimicrobial effects of the sealers
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Antibacterial effects of conventional glass ionomer cement
(Comenius University, Faculty of Medicine, Bratislava, Slovakia, 2016)
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Nicholson, JW
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OBJECTIVES: The antibacterial activity of conventional glass ionomer cement against three different microorganism strains alone and following incorporation of 1, 2 and 3 % Benzalkonium Chloride and Cetylpyridinium Chloride was evaluated. METHODS: Agar diffusion method was used to determine the inhibitory effect of the conventional glass ionomer cement ChemFlex on Streptococcus mutans, Lactobacillus casei and Actinomyces viscosus. Bacterial strains were inoculated into BHIB, and incubated in an anaerobic atmosphere (37 °C). From the bacteria grown in the liquid medium, the density of the inoculum was set to be equivalent to McFarland 2 standard. In Shaedler agar, 350 μL of the bacterial suspension were equally spread. Specimens (4 mm × 6 mm) were prepared from the cement without and with addition of 1, 2 and 3 % Benzalkonium Chloride and Cetylpyridinium Chloride. The inhibition zones were determined after 48 hours, after 2, 7 and 21 days of incubation. RESULTS: The combination ChemFlex + Benzalkonium Chloride has the best effect on the three analysed bacteria. The Benzalkonium Chloride antibacterial compound has a stronger antibacterial effect than Cetylpyridinium Chloride. CONCLUSIONS: Glass ionomer cements can potentially be used as a medium for slow release of active antimicrobial components, and they have the potential to improve clinical outcomes of the cements
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Impact of integrated quaternary ammonium compounds in conventional glass-ionomers on cariogenic oral microflora: An in vitro analysis
(Maad Rayan Publishing Company, 2025-09-30)
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Simonoska, Jasna
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Efficacy of Laser Pulpotomy vs. Conventional Vital Pulpotomy in Primary Teeth: A Comparative Clinical Analysis
(MDPI AG, 2025-03-08)
Simonoska, Jasna
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Bjelica, Roko
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Simjanovska, Jasmina
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Gabrić, Dragana
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New findings about releasing of chloride ions and quaternary ammonium compounds from conventional and experimental glass ionomers
(Termedia Sp. z.o.o., 2024)
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Simonoska, Jasna
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A review of recent advances in paediatric dentistry
(Centre for Evaluation in Education and Science (CEON/CEES), 2024)
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The Impact of Triclosan-Containing Toothpaste on the Overall and Cariogenic Salivary Microbiota Among Primary School Children
(Athenaeum Scientific Publishers, 2024-11-05)
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The Role of Preventative Dental Teams in Maintaining Dental Health Education in Children of Preschool Age
(Termedia Sp. z.o.o., 2024)
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Simonoska, Jasna

Dimkov, Aleksandar

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