Petkov, Vladimir
Preferred name
Petkov, Vladimir
Official Name
Petkov, Vladimir
Main Affiliation
Email
petkov@fvm.ukim.edu.mk
6 results
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Item type:Publication, IMPACT OF MAMMARY GLAND INFECTIVE STATUS ON THE CONCENTRATION OF LACTOFERRIN IN COW’S MILK(JHED, 2021) ;Trajkovska, Biljana ;Kochoski, Ljupche ;Petrovski, Kiro ;Dimitrovska, GordanaLactoferrin (LF) is glycoprotein, which is normally excreted in cow’s milk, but as a result of inflammatory processes in the mammary gland, its concentration is being increased. The aim of this research was to determinate whether the LF concentration in milk is influenced by increased number of milk somatic cell count. In this research we used milk from (n = 345) Holstein- Friesian cows in Pelagonia region, or more precisely milk from healthy (n = 112) and infected quarters (n = 233). A quarter was classified as healthy when no visible signs of mastitis were detected or the California Mastitis Test (CMT) was negative. A quarter was classified as subclinical when no visible signs of mastitis were detected but CMT was positive. A quarter was classified as clinical mastitis when there were visible signs of mastitis (cow, udder or milk appearance). The milk samples were analysed instrumentally for physico - chemical properties (Lactoscope C4+), somatic cell count (SCC) by Bentley Somacount CC 150, total number of bacteria (CFU) (by Bentley Bactocount IBC), and by ELISA for concentrations of lactoferrin. We used SPSS 15.0 for analysis of gained data. Significant differences were detected in concentrations of lactoferrin in healthy quarters (915.94 μg/mL) and in quarters with sub-clinical (2,095.87 μg/mL) and clinical mastitis (3,528.99 μg/mL) (p < 0.001). A correlation between lactoferrin and lactose (r = -0.21, p < 0.01), lactoferrin and CFU (r = 0.28, p < 0.01) as well as between lactoferrin and somatic cells (r = 0.32, p < 0.01) was observed. These results initiate that lactoferrin could be used as complementary test to somatic cell count, and potential to be a primary diagnostic test for subclinical and clinical mastitis in dairy cows. Furthermore, it could be used as natural antibiotic in treatment of mastitis in dairy cows, in order to decrease the presence of antibiotic residuals in milk, thereby to improve hygiene of raw milk. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Homologous Seminal Plasma and Glutathione Promote Pre-Capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa(Macedonian Veterinary Review, 2019-09); ;Monika Dovenska; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, CHANGES IN THE LACTOFERRIN CONCENTRATION AT DRYING OFF PERIOD(“St. Kliment Ohridski” University – Bitola, 2021) ;Trajkovska, Biljana ;Kochoski, Ljupche ;Dimitrovska, Gordana; In the lactation cycle of dairy cows, the dry off is a critical period of rest for the udder, in order to optimize milk production in the next lactation. Lactoferrin (LF) is an iron-binding protein normally excreted in cow’s milk and it is one of the natural protective factors having the ability to inhibit the growth of mastitis-causing pathogens. The objective of this research was to determine whether LF concentration in the milk is increased at drying off period. Quarter milk samples (n=40) from clinically healthy Holstein-Friesian cows were taken. Milk samples were collected one week prior to dry off and on the day of dry off. The milk samples were analyzed for physicochemical properties, somatic cells (SCC) and concentrations of lactoferrin. The mean LF concentration in the milk was found to increase significantly (p<0,05) from 2.977,28 μg/mL, one week prior to dry off to 4.529,07 μg/mL on the day of drying off process. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, CHANGES IN THE LACTOFERRIN CONCENTRATION IN THE BOVINE COLOSTRUM DURING POSTPARTUM PERIOD(University Ss Kiril and Metodij, Faculty of Veterinary Medicine, 2022-09-11) ;Trajkovska, Biljana ;Kochoski, Ljupche ;Dimitrovska, Gordana; The health condition of the mammary gland is highly crucial for obtaining good quality colostrum. The lactoferrin (LF), as an iron-binding protein, plays a key role in the defense mechanisms of the mammary gland due to its’ bacteriostatic properties. The study aimed to investigate the factors affecting the LF concentration in the bovine colostrum milk during the postpartum period, and the effect of somatic cell count (SCC) on the LF concentration and milk composition. The cows were randomly selected at a dairy farm in Pelagonia region. Colostrum samples from 12 cows were collected. Samples were taken at different time intervals after parturition, 1, 6, 12, 24, 48, 72, 96, and 120 h after parturition. Cows in their second parity were found to have higher levels of LF compared to cows in the third and higher parity (p>0.05). Time had a significant effect on the LF concentration (p<0.01), with the highest value recorded 1 h after parturition remaining stable for one hour. LF concentration and SCC in the colostrum showed a weak positive correlation (r=0.40; p<0.01). The highest LF concentration was noticed when SCC was above 800,000 cells/mL. In conclusion, the colostrum LF concentration was significantly affected by the interactions of the two factors, i.e., time after parturition and SCC (p<0.05). Milk composition was affected by elevated SCC. Parity showed no association with LF. The SCC can be used as a reliable means of assessing colostrum quality. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Novelties in Ovine Assisted Reproductive Technologies – A Review(Walter de Gruyter GmbH, 2022-04-27); ;Trojacanec, Plamen; ; <jats:title>Abstract</jats:title> <jats:p>Artificial insemination (AI) as a part of assisted reproductive technologies represents the oldest and most widespread method used to accelerate genetic progress in all domestic animals. After its first implementation in ovine reproduction and almost 80 years afterward, AI is continuously used for improving the genetic merit, utilizing either fresh or short-time chilled semen. Nevertheless, regardless of the semen used for insemination, the conception rate (CR) is still lower in comparison to natural service. At least two factors are commonly thought to limit the success of the AI and reduce the CR: (1) failure of placing the semen directly into the uterus due to the specific anatomic structure of the ewe’s cervix; (2) lower viability of ram spermatozoa during cryopreservation (<30% progressively motile spermatozoa after thawing). This review elaborates on recent studies that aimed to achieve acceptable CR through the implementation of cervical or intrauterine insemination: deep intracervical, intrauterine trans-cervical, and intracornual. Several hormonal treatments (oxytocin, estrogen, or prostaglandin) were evaluated on inducing cervical dilation that facilitates insemination. A comprehensive analysis was given to the effects of several antioxidants (GSSG, GSH, and cysteine) supplemented in ram semen-freezing media. Sex-sorted ram semen fertility rate results were presented from our studies.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Ram spermatozoa motile and metabolic activity – potentially predictable pattern of ejaculate fatty acid composition(COST EPICONCEPT Workshop 2015 Periconception EnvironmentAt: Dubrovnik, Croatia 26 - 29 April 2015, 2015-04); ;Monika Dovenska; ;
