Faculty of Medicine

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Language: search.filters.language.EnglishSubject: search.filters.subject.SDS-PAGE

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    Value of sodium dodecyl sulfate polyacrilamide gel electrophoresis in evaluation of pediatric urinary tract infections
    (SHMSHM/AAMD, 2017)
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    Alulovska N.
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    Urinary tract infections (UTI) represent frequent health problem, especially in developing countries. Clinical signs are different based on the location of infection. An interesting ethical and professional question arises: how to treat febrile children who previously received antibiotic therapy and have sterile urine culture in presence of pyuria. Is that a pyelonephritis and are further invasive imaging studies needed? Objectives: To analyze the electrophoretic patterns of urinary proteins in children with proximal respectively distal UTI and to evaluate the value of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in acute febrile respiratory infections (AFRI) without signs and symptoms of UTI. Material and methods: The study included 79 children aged 1-16 years: 36 with proximal UTI (15 with culture positive APN, respectively 21 with culture negative APN), 23 with cystitis (7 with hemorrhagic cystitis) and 20 children with AFRI without symptoms of UTI. Separation of urinary proteins was performed by SDS-PAGE. Results: Children with culture positive and culture negative APN had incomplete tubular proteinuria (24-67 kDa) which was transient. Hemorrhagic cystitis was characterized by the typical presence of 28 kDa band (apolipoprotein A-1). In the group AFRI without symptoms of UTI, there was no observed pathological electrophoretograms. Conclusions: SDS-PAGE is useful laboratory technique for separating proteins by their molecular size. The presence of incomplete tubular proteinuria indicates the renal origin of the fever. Detection of apolipoprotein A-1 in children with hemorrhagic cystitis allows differentiation in relation to glomerular cause bleeding. Despite these facts, in the literature there are few reports on SDS-PAGE in diagnosis of various renal diseases including urinary tract infections.
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    The Spectrum of Kidney Diseases in Children Associated with Low Molecular Weight Proteinuria
    (ID Design 2012/Scientific Foundation SPIROSKI, 2018-05-20)
    Shpetim Salihu
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    Natasa Aluloska
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    Proteinuria, in addition to haematuria, is the most important laboratory parameter in patients with nephro-urological diseases. Low molecular weight proteinuria (LMWP) is of particular importance because some diseases genetic and tubulointerstitial are diagnosed based on its presence.
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    Incidental Detection of Dent-2 Disease in an Infant with Febrile Proteinuria
    (S. Karger AG, 2018)
    Shpetim Salihu
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    Febrile proteinuria is functional proteinuria and is seen as a transitory phenomenon during acute febrile illness, mainly viral infections. It is a benign phenomenon and clears promptly with resolution of the infection.
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    Low Molecular Weight Proteinuria in Children with Distal Renal Tubular Acidosis
    (Walter de Gruyter GmbH/Macedonian Academy of Sciences and Arts, 2018-07-01)
    Shpetim Salihu
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    Momir Polenakovic
    Distal renal tubular acidosis (dRTA) (MIM #267300, #602722 and #179800) is a rare inherited tubulopathy characterized by the inability of the distal tubule to acidify the urine with consecutive systemic acidosis. The clinical features include polyuria, polydipsia, poor appetite, failure to thrive, short stature and rickets. Prominent biochemical features are hypokalemia, hypercalciuria and hypocitraturia. There are reports on patients who presented with unusual biochemical features such as low molecular proteinuria, hypophosphatemia, hypouricemia, generalized hyperaminioaciduria, hyperoxaluria and other making diagnostic confusion to the clinicians. In this work, we report on a series of 8 children with clinically, biochemically and genetically proven dRTA who present with low molecular proteinuria at the disease onset. With metabolic compensation of the disease, there was complete resolution of the low molecular weight protenuria and other proximal tubular abnormalities in all children. Late recognition of the disease with long standing hypokalemia and acidosis may result in abnormal expression and function of the transporters in the proximal tubules. Sodium dodecyl sulphate polyacrylamide gel electrophoeresis is an accurate method for detection and follow up of patients with low molecular weight proteinuria.</jats:p>