Faculty of Medicine

Permanent URI for this communityhttps://repository.ukim.mk/handle/20.500.12188/14

Browse

Settings

Has files: YesSubject: search.filters.subject.interleukins

Search Results

Now showing 1 - 3 of 3
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Correlation of serum levels of inflammatory cytokines with severe form of cholecystitis
    (Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 2022)
    Spasovski, Zharko
    ;
    ;
    Novevska Petrovska, Biljana
    ;
    Stojanoski, Sashko
    ;
    Introduction: Cholecystitis is an inflammatory response of the body triggered by a number of mutually supporting biological mechanisms, which by creating and releasing inflammatory mediators - cytokines activate the innate or acquired immune system, which leads to neutralization of the harmful stimulus and initiation of the process of repair and regeneration of damaged tissue or its continuation as a long-term chronic process with simultaneous tissue destruction and reparation. Material and methods: The study was conducted at the General City Hospital (GCH) "8th September" in Skopje and the Institute of Immunology and Human Genetics in Skopje, in the period of 2020-2022. Statistical analysis of the data was performed with the statistical package SPSS for Windows 26.0. Results: The study included 165 subjects with gallbladder inflammation divided into 3 groups: mild, moderate and severe inflammation grade. Patients with mild, moderate, and severe inflammatory processes differed significantly in IgG levels (p = 0.049), IgA (p = 0.021), and IgM (p = 0.016) and insignificantly in IgE1 levels (p = 0.16). Patients with a severe inflammatory process had a higher prevalence of IL-2R and IL-8 than patients with a mild grade (p = 0.035; p = 0.26, respectively). The intensity of inflammatory process had a nonsignificant effect on the levels of TNF-alpha (p = 0.078), and a significant effect on the levels of fibrinogen (p = 0.001), with significantly higher levels of fibrinogen in the group of patients with severe inflammatory process compared to the group with mild grade (p = 0.0009). Conclusion: The intensity of inflammatory process affects the serum levels of inflammatory cytokines with presence of strong correlation between the severe form of cholecystitis and elevated serum levels of certain inflammatory cytokines.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    The Role of Interleukin-1 and Interleukin-6 in the Development of Acute Myocardial Infarction
    (2023-08-26)
    ;
    Elizabeta Srbinovska-Kostovska
    ;
    ;
    Slavica Josifovska
    ;
    Certain cytokines as interleukin-1 (IL-1) and interleukin-6 (IL-6) are produced by the neutrophils and macrophages localized in the atheromatous plaques. The levels of circulating inflammatory cytokines IL-1 and IL-6 are found to be significantly increased in patients with ST-Segment Elevation Acute Myocardial Infarction (STEMI). In a group of 38 patients with STEMI, the levels of IL-1ß and IL-6 were determined in the coronary circulation (CC) by analysis of aspirates from the culprit lesions and the levels of these inflammatory markers in the systemic (peripheral) circulation (SC) during STEMI and six months after STEMI. Coronary angiography (CA) and percutaneous coronary intervention (PCI) were performed in all patients and the levels of cytokines were determined by the ELISA method. By examining the levels of IL-1ß and IL-6, the aim of this study was to determine their predictive value in short-term prognosis. In the acute phase (STEMI) there was a statistically significant difference between the mean values of IL-1ß (p=0, 000000) and IL-6 (p=0, 026204) in the samples of CC and SC, respectively. Results from the six months of follow-up showed that there were statistically no significant differences between the mean values of IL-1ß and IL-6 in the CC and SC. Moderate and low-positive correlation between the mean values of IL-1ß (r=0,6816; p=0,000) and IL-6 (r=0,4291; p=0,000) in CC and SC was observed at the sixth month of follow-up. In conclusion, our results confirm that IL-1ß and IL-6 are linked to the progression of Coronary Artery Disease (CAD) and should be considered as predictive markers.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Correlation between IL-6 (Interleukin 6) and Laboratory Parameters (CRP, Total Proteins and LDH) for Verification of the Inflammatory Origin in Preeclampsia
    (SHMSHM / AAMD, 2020-03)
    ;
    ;
    Mirjana Kaeva Pejkovska
    In normal pregnancy there is a transfer to the Th2 anti-inflammatory type of immunity, directed toward the fetus and the placenta, whereas in pregnancy complications, such as preeclampsia due to absent transfer, Th1 or proinflammatory immune system is dominated. This article analyzes what are the most important circulating biomarkers created by syncytiotrophoblast that together combined and promote oxidative stress. An increase in proinflammatory and decline in the antiinflammatory cytokine is an imbalance that explains the theory that due to impairement of the immunological response, consequences on the mother's circulation are developed and are reflected in the manifestation of pregnancy complications such as preeclampsia and intrauterine growth restriction. The purpose of the study is to emphasize the importance of the results of the correlation between interleukin 6, measured in the serum of patients from 14 to 20 gestational weeks, with laboratory parameters that are pathognomonic for preeclampsia (LDH, total protein and CRP). This explains the immunological and inflammatory response in preeclampsia serving as a predictor of the condition. For that purpose, the materials and methods that were used were 100 patients examined at the University Clinic of Obstetrics and Gynecology, divided in two groups. The first or examined group consists of 50 patients with notch of the uterine artery present in the second trimester from 14 to 20 gestational weeks. The second, or control group consists of 50 patients with absent notch of the uterine artery. These patients are then referred to the Institute of Immunobiology and Human Genetics at the Faculty of Medicine in Skopje to examine the levels of cytokines, in particular the relationship between the proinflamatory with antiinflammatory cytokines (TNF-α, IL-6 vs. IL-10) using ELISA (enzyme-linked immunosorbent assay) methodology, with Magnetic Luminex Assay multiplex kit. The results between the examined and the controlled group verified an increase in proinflammatory (TNF-α and IL-6) and a decrease in the antiinflamatory cytokine (IL-10), from which the variance of the level IL-6 showed important statistical significance p<0.01. Positive correlation was of great statistical significance between levels of cytokine IL-6 and increased levels of LDH and CRP, and with decreased levels of the total proteins of the preeclamptic patients. (LDH p<0.00001, CRP p<0.025 and total proteins p<0.0012). The role of the inflammatory response is obtained by the correlation of the interleukins in the second trimester and laboratory examinations in the third trimester as a sufficient predictive method in detection in early stages the preeclampsia syndrome.