Faculty of Medicine
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Item type:Publication, ROLE OF LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 (LP-PLA2) IN THE PREDICTION AND ASSESSMENT OF THE SEVERITY OF CORONARY ARTERY DISEASE IN PATIENTS WITH TYPE 2 DIABETES MELLITUS(Society of Medical Biochemists of Serbia, Belgrade, 2025-05); ; ; Background: Lipoprotein-associated phospholipase A2 (LpPLA2) is a specific biomarker associated with an increased risk of coronary artery disease (CAD) development. This study aims to determine the relationship between Lp-PLA2 activity and the risk of development and severity of CAD in patients with type 2 Diabetes mellitus (T2DM). Methods: The cross-sectional study included 148 patients with T2DM, divided into two groups: patients with T2DM without confirmed CAD (n=56) and patients with T2DM and confirmed CAD (n=92), further divided into three subgroups based on the stage of CAD, and a control group of healthy individuals (n=44). Venous blood samples were collected from all participants to measure glucose, cholesterol, triglycerides, HDL, LDL, C-reactive protein, urea, and creatinine levels using standard photometric methods. LpPLA2 activity was measured using a chemiluminescent immunoassay method.Results: Patients with T2DM and confirmed CAD had significantly higher Lp-PLA2 levels than those without confirmed CAD and healthy individuals. A significant difference in LpPLA2 levels was found between the group without CAD, the patients with CAD divided into subgroups according to disease stage, and the healthy control group. A positive correlation was observed between Lp-PLA2 and BMI, glycated haemoglobin, total cholesterol, and HDL cholesterol. The optimal cutoff value for Lp-PLA2<250 ng/mL yielded a diagnostic sensitivity of 95.65% and specificity of 88.64% for patients with T2DM and diagnosed CAD. Conclusions: Lp-PLA2 can be used as a predictor for developing and assessing the severity of CAD in patients with T2DM. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Carotid intima media thickness is in a relation to risk factors for coronary artery disease(Geotar-Media Publishing Group, 2020-01); ; Introduction: Carotid intima-media thickness (CIMT) is a double line pattern measured from the lumen-intima to media-adventitia interface. Measuring CIMT with B-mode ultrasound can detect early arterial wall changes. We aimed to measure CIMT in manifested atherosclerotic disease and find a relation to present risk factors. Material and methods: This cross sectional study included a population of 657 patients, with documented coronary artery disease (CAD), 66,1% were men (469 pts), 33,9% were women (241 pts). The mean age of patients was 64,72 years (55,6-73,8). The measurement of CIMT (maximal and mean) was performed with B-mode ultrasound, on longitudinal view, on multiple plaque-free segments at the level of common carotid artery, on the far wall, on both sides. Multivariate regression analysis was done to estimate independent factors for CIMT, when risk factors, age, and sex included in analysis. Results: The mean value of maximal CIMT was 0,96 mm (0,52-1,4), mean value of mean CIMT was 0,88 mm (range 0,61-1,15) and mean systolic blood pressure of 133,21 mm Hg (108,03-158,39) was found. Increased CIMT was found in 75,7% of patients (502 pts). Smoking was found as an independent risk factor for increased CIMT with odds ratio (OR) 4,7 (95% CI; 0,67-5,32) and diabetes mellitus with OR 1,6 (95% CI; 0,47-2,16). Conclusion: Diabetes and smoking are independently related to increased CIMT. These are preliminary results of National survey on 657 pts with coronary artery disease. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Association of PvuII polymorphism in the lipoprotein lipase gene with the coronary artery disease in Macedonian population(Macedonian Academy of Sciences and Arts, 2008); ; Sadikario, SamuelIn the etiology of coronary artery disease there are many factors involved as a result of the complex interaction between genetic predisposition and environmental influences. The lipoprotein lipase (LPL) plays a very important role in lipid metabolism. It hydrolyzes the triglycerides in hylomicrones and very low density lipoproteins - VLDL. PvuII polymorphism in the LPL gene is a frequent variant and it increases triglyceride levels and the risk of the appearance of coronary arterial disease. Aim: The aim of this work is to show LPL-PvuII polymorphism as an independent risk factor and also as a predictor of coronary arterial disease in the Macedonian population. Material and methods: The study included 109 randomized patients with coronary artery disease (CAD) (83 males, 26 females), treated at the Cardiology Clinic. The stenosis of coronary arteries greater than 70% of the artery lumen was angiographically documented in the CAD group. The control group consisted of 32 patients (25 males, 7 females) with documented normal coronarographic findings. The patients' age ranged from 50 to 59; the mean age in the CAD group was 59.4 and the mean age in the control group was 57.9. LPL-PvuII polymorphism in the intron 6 in the CAD and control group was detected by PCR amplification and restriction enzyme digestion. Results: A statistically significant association between CAD and the control group was found regarding the presence of hyperlipidaemia (p < 0.001), diabetes (p < 0.05) and the use of antilipidaemic drugs (p < 0.049). The presence of LPL-PvuII polymorphism in both investigated groups does not represent a statistically significant risk factor for the appearance of coronary artery disease (p = 0.816). The PvuII + allele frequency of 0.495 and 0,469 was obtained in both the angiographically confirmed CAD and the control groups, respectively. This finding indicates no significant differences between the prevalence of the LPL-PvuII genotypes in both study groups, suggesting a lack of association of LPL-PvuII polymorphism with CAD. However, the homozygous genotype (PvuII +/+) was more prevalent in the CAD group (22.9%) in comparison with the control group (15.6%). Conclusion: In our study LPL-PvuII polymorphism was not identified as an independent risk factor for the appearance of CAD. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, ASSOCIATION OF LPL-HINDIII POLYMORPHISM WITH CORONARY ARTERY DISEASE IN MACEDONIAN POPULATION(Association of medical doctors „Sanamed” Novi Pazar, 2013)Objective: Coronary artery disease (CAD) is a leading cause of high mortality and morbidity in worldwide. The HindIII polymorphism of the LPL gene (LPL-HindIII) is a common variant and has been associated with plasma lipid and lipoprotein variability in population studies. Aim: Evaluation of the LPL-HindIII polymorphism as an independent risk factor for coronary artery disease in Macedonian population. Material and Methods: Apolymerasechainreaction amplification and consecutive restriction enzyme digestion was used to reveal lipoprotein lipase, the intron 8 LPL-HindIII polymorphism. Study group included 114 randomized subjects with angiographically documented coronary artery stenosis (CAD group: 87 males, 27 females). Control group consisted of 35 patients (21 males and 14 females) without significant stenosis in coronary arteries. Results: Independent multiple regression analysis of LDLplasma level and their correlation with LPL-HindIII polymorphism and analyzed risk factors: hypertension, diabetes, family history of CAD, physical activity, antilipidemic drugs and alcohol consumption, LDL, show statistically significant correlation with BMI, and also between LPL-HindIII and LDLplasma level. In the examined group, only triglycerides reached a statistically significant association with the LPL-HindIII polymorphism. Conclusion: In our study, the LPL-HindIII polymorphismwasnotidentified as independent risk factor for CAD, but showed association with high triglycerides and LDLlevels. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, FREQUENCY OF GENETIC VARIANTS ASSOCIATED WITH CORONARY ARTERY DISEASE AND VENOUS THROMBOEMBOLISM IN YOUNG PATIENTS IN REPUBLIC OF N. MACEDONIA(Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 2022); ; ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, SERUM ANTI-OXLDL ANTIBODIES IN PATIENTS WITH ANGRIOGRAPHICALLY CONFIRMED CORONARY ARTERY DISEASE(Macedonian Association of Anatomists and Morphologists, 2023-12); ; ; Cardiovascular disease (CVD) remains the leading cause of death worldwide. Oxidized low density lipoprotein (oxLDL) is believed to be central to the atherosclerotic cascade. Oxidative modification of LDL induces immunogenic epitopes in the LDL molecule, and the presence of antibodies against oxidized LDL (anti-oxLDL) has been demonstrated in human sera. Anti-oxLDL titer not only can predict a presence of atherosclerotic CAD but may also be a marker of plaque instability. The primary aim of this study was measurement of serum anti ox-LDL concentrations in CAD patients confirmed with coronary angiography. The secondary aim of the study was to evaluate if there is an association between anti-ox-LDL concentration and conventional risk factors for CAD. Ninety patients with coronary artery disease and 90 controls were included in the study. Patients were selected according to the positive result of coronary angiography. Total cholesterol, triglycerides, HDL-c and LDL-c were determined on Roche C311 Cobas Analyzer and OxLDLD and anti-oxLDL were determined with sandwich ELISA technique. We find significant difference between CAD patients and controls regarding OxLDL (p< 0.001). Serum anti OxLDL antibodies were correlated with LDL-c and we found significant correlation (p<0.05) in the CAD group. The regression model showed that the variables Age, BMI, DBP, SBP, Total cholesterol, HDL-c LDL-c and TG explained 30.39% of the variance from the variable anti-ox-LDL. Our results show that serum levels of ox LDL are associated with presence of CAD and with the lipid parameters. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, The Role of Interleukin-1 and Interleukin-6 in the Development of Acute Myocardial Infarction(2023-08-26); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Association of the polymorphism rs3918242 of the matrix metalloproteinase-9 gene with coronary artery disease in a younger population(Macedonian Academy of Sciences and Arts, 2023-06); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, HOMOCYSTEINEMIA AND GENES MUTATION IN CORONARY ARTERY DISEASE(Croatian Laboratory Associoation, 2019-10); ; ; ; Zumberi, Nagip - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Determination of asymptomatic endothelial dysfunction in coronary artery disease in patients with seropositive rheumatoid arthritis with asymmetric dimethylarginine (adma) and myocardial perfusion scintigraphy(Sievert Association, 2021-09); ; To compare the values and accuracy of the test of Asymmetric dimethylarginine (ADMA) assessed with myocardial perfusion scintigraphy (MPS), acute phase reactant, C - reactive protein (CRP) and disease activity index (DAS 28) in early diagnosis of untreated Rheumatoid arthritis (RA). To determine whether ADMA changes depend on the disease evolution. ADMA is used as an indicator for endothelial dysfunction.