Faculty of Medicine

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    PREVALENCE OF AUTOANTIBODIES AGAINST THE PLATELET GLYCOPROTEIN COMPLEXES GP IIb/IIIa, GP Ib/IX AND GP Ia/IIa IN PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA
    (Македонско лекарско друштво = Macedonian Medical Association, 2017)
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    Stojanovic A
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    Efremov D
    Chronic lymphocytic leukemia (CLL) is a malignant disorder characterized by the frequent occurrence of autoimmune hemolytic anemia (AIHA) and autoimmune idiopathic thrombocytopenic purpura (ITP). In this study we analyzed the presence of auto-Abs against the platelet GP complexes GP IIb/IIIa, GP Ib/IX and GP Ia/IIa in 55 consecutive CLL patients using a new antigen specific assay. All patients were simultaneously tested for anti-RBC auto-Abs with the direct antiglobulin test. Antiplatelet auto-Abs against one or more GP complexes was detected in 25 patients (45%). Anti-RBC auto-Abs were detected in 18 patients (33%), 6 of them (11%) had evidence of AIHA at the time the sample was taken. The obtained data show that platelet specific Abs develops frequently in patients with CLL, even more often than anti-RBC auto-Abs. The presence of platelet specific auto-Abs together with thrombocytopenia and normal Hb levels in 16% of patients indicated that ITP may be more common cause of thrombocytopenia in CLL than previously anticipated.
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    BCR-ABL transcripts are not expressed in essential thrombocytemia.
    (2001)
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    Crcareva A
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    Siljanovski N
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    Molecular monitoring and BCR-ABL gene mutations in Imatinib resistant CML patients in our centre.
    (2019)
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    Plasevska-Karanfilska D
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    Angelovic R
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    Angelkovic E
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    LONG-TERM FOLLOW-UP OF ADULT PATIENTS WITH IDIOPATHIC THROMBOCYTOPENIC PURPURA AFTER SPLENECTOMY
    (2011-09)
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    Trpkovska-Terzieva S
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    Latifi A
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    Background: Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disease characterized by isolated thrombocytopenia and the absence of any underlying cause for thrombocytopenia. Corticosteroids are the standard first line treatment for patients with symptomatic disease, but in many cases, steroid tapering or withdrawal is followed by a decrease of platelet count and the need for additional treatment. Splenectomy is still the standard salvage therapy in cases refractory to corticosteroid therapy. Aim: The aim of this study was to evaluate the long-term outcome of splenectomized patients with ITP. Materials and Methods: We retrospectively analyzed medical records of 38 patients with ITP that underwent splenectomy after first-line steroid treatment. All patients were followed for at least one year. Results: According to the results at the time of the last control, 28 patients had complete response (CR), but from those 28 patients only 22(58%) were without therapy and 6(22%) were receiving prednisone, azathioprine or both. These results indicate that only 22/38(58%) of patients had long-lasting CR without therapy, 12 patients (31%) were receiving therapy and 4 patients (11%) had partial response without therapy. Conclusion: In conclusion, splenectomy may be considered as safe and effective treatment for patients with ITP who failed to respond to firs-line treatment with corticosteroids.
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    Evaluation of telomerase activity in patients with chronic B lymphocytic leukemia versus age matched controls. Correlation between telomerase activity and bone marrow infiltration
    (Македонска академија на науките и уметностите, Одделение за биолошки и медицински науки = Macedonian Academy of Sciences and Arts, Section of Biological and Medical Sciences, 2007)
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    Stojanovic A
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    Telomerase is a ribonucleoproteic enzyme associated with cellular immortality and malignancy. This enzyme, besides the catalytic subunit bearing reverse transctiptase activity, contains an RNA template complementary to TTAGGG telomeric repeats, thus permitting de novo synthesis of telomeric DNA onto chromosomal telomeric ends. Increased telomerase activity has been reported in Chronic Lymphocytic Leukemia (CLL) by many authors. In order to investigate the telomerase activity in patients with CLL and its correlation to commonly used morphologic prognostic markers, 38 frozen blood lymphocyte samples from patients with CLL and 47 age-matched controls were investigated for telomerase activity using the Telomerase PCR ELISA-plus kit from Roche. Trepanobiopsies from the same patients were analysed for the type of bone marrow infiltration as well. Analysis showed highly variable Relative Telomerase Activity (RTA) in B-CLL patients, ranging from comparable or even lower than the mean RTA of controls (in Binet A stage patients) to manifold increase in the majority of patients with advanced stage disease. The sex and age of the patients showed no influence on RTA in CLL patients, in contrast to the control group, where the age influenced telomerase activity. We found a positive correlation between the RTA and disease stages (Binet), as well as between RTA and the type of BM infiltration.