Faculty of Medicine

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Access type: UnknownAuthor: search.filters.author.Nikodijevic, Dijana

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    Validation of the ELISA Method for Quantitative Detection of TNF-α in Patients with Intracerebral Hemorrhage
    (Scientific Foundation SPIROSKI, 2017-09)
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    Aleksovski, Boris
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    Stojanov, Dragan
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    Mihajlovska-Rendevska, Ana
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    Aleksovski, Vasko
    <jats:p>AIM: We aimed to investigate the sensitivity, reproducibility and validity of the commercial ELISA kits for quantitative detection of TNF-α and their potential application for screening purposes in patients with ICH.METHODS: Analysis of six independent standard series, evaluation of the deviation of the TNF-α concentration in patients with ICH, standard addition and visual analysis of whole UV-Vis spectra were carefully performed.RESULTS: Low standard deviations of the absorbance were detected for every standard, as well as in the samples of healthy controls and patients with ICH. The standard addition series have also confirmed high sensitivity and reproducibility of the assay, with a congruent shift of the standard curves with the concentration of TNF-α for the added plasma. The visual analyses of the gained spectra have revealed the absence of any matrix effects from the addition of the human plasma in the reconstituted standards.CONCLUSION: The commercial ELISA kits can be used in the clinical practice for screening purposes of the plasma TNF-α levels in patients with ICH.</jats:p>
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    Cysteinyl leukotrienes and hematoma-correlation study
    (League against Epilepsy of Macedonia, 2016)
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    Nikodijevic, Dijana
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    Baneva, Emilija
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    Spectrum of functional neurological disorders
    (2018)
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    Medjiti, Fatmir
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    Mitrevska-Velkov, Jasmina
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    Intracerebral Hematoma, Perihemorrhagic Edema and Urinary Excreted Cysteinyl Leukotrienes Correlation Study
    (Walter de Gruyter GmbH, 2014-08-01)
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    Nikodijevic, Dijana
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    <jats:title>Abstract</jats:title><jats:p><jats:bold>Introduction.</jats:bold>Several mechanisms in formation of perihemorrhagic edema are activated after contact of brain tissue-extravasated blood in intracerebral hemorrhage. Cysteinyl leukotrienes (cysLT) (C4, D4, E4) are included in this process as significant edema factors and they determine the neurological deficit and outcome. The study aim was a 5-day follow-up (admission/3 day/5 day) of urinary cysLT, hematoma volume, edema volume values and their correlation in patients after spontaneous, primary supratentorial intracerebral hemorrhage.</jats:p><jats:p><jats:bold>Methods.</jats:bold>An enzyme immunoassay was used for urinary cysLT measured in 62 patients and 80 healthy controls. Hematoma and edema volumes were visualized and measured by computed tomography and mathematically calculated with a special spheroid shape formula (V=AxBxC/2).</jats:p><jats:p><jats:bold>Results.</jats:bold>CysLT of hemorrhagic patients (1842.20±1413.2, 1181.54±906.2, 982.30±774.2pg/ml/mg creatinine) were significantly excreted (p<0.01). Brain edema (12.86±13.5, 22.38±21.1, 28.45±29.4cm<jats:sup>3</jats:sup>) was significantly increased (p<0.01). Hematoma volume values (13.05±14.5, 13.13±14.7, 12.99±14.7cm<jats:sup>3</jats:sup>) were not significant (p>0.05). A high correlation (multiple regression) between cysLT, hematoma and edema was found on the 3<jats:sup>rd</jats:sup>day (R=0.6) and a moderate correlation at admission (R=0.3) and on the 5<jats:sup>th</jats:sup>day (R=0.3).</jats:p><jats:p><jats:bold>Conclusion.</jats:bold>In our 5-day follow-up study a significant cysLT brain synthesis and significant brain edema progression versus constant hematoma volume values in hemorrhagic patients was found. A high correlation between cysLT, hematoma and edema volume was found on the 3<jats:sup>rd</jats:sup>day, a moderate correlation on admission and on the 5<jats:sup>th</jats:sup>day, which means that high cysLT and hematoma values were associated with high/moderate edema values.</jats:p>
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    Refractory Epilepsy-MRI, EEG and CT scan, a Correlative Clinical Study
    (ID Design 2012/DOOEL Skopje, 2016-03-15)
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    Refractory epilepsies (RE), as well as, the surgically correctable syndromes, are of great interest, since they affect the very young population of children and adolescents. The early diagnosis and treatment are very important in preventing the psychosocial disability. Therefore MRI and EEG are highly sensitive methods in the diagnosis and localization of epileptogenic focus, but also in pre-surgical evaluation of these patients. The aim of our study is to correlate the imaging findings of EEG, MRI and CT scan in refractory symptomatic epilepsies, and to determine their specificity in detecting the epileptogenic focus.