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    HONEY BEE HEALTH STATUS IN BULGARIA AND MACEDONIA, EVALUATION OF RISK
    (Institute of Knowledge Management, Skopje, 2017-03-31)
    Valtchovski Rumen
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    Hristovska Galina
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    Valchovski Petar
    Abstract: Today it is well known that beekeeping occupies one of the most important stakes in global agriculture.Besides the obvious contribution to the receipt of bee products (honey, pollen, wax, propolis and etc.), bees are one of the most effective natural pollinators of a wide variety of wild flora and crops. This activity led to thepreservation of biodiversity and improving the balance of the ecosystem. In the world as a whole the total economicvalue of pollination and bee products from beehives worth over 150 million euros a year (Gallai et al., 2008). To besuccessful this activity is particularly important to their health status. The mysterious mass extinction of bees, for which destroyed about a third of bee colonies every year since 2006, may be due in addition to the well-known Paenobacillus larvae and P. alvei, nosema, also of not a harmless part of viruses. Recent studies show an increase in mortality in winter, the 2016 and 2017, the and led to concerns about the continuation of the downward trend of bee population. We made a risk analysis of economically significant diseases in bees in Bulgaria and Macedonia. We believe that such analyzes have been made ahead of time for them this presents various issues related to the changed picture of the health of bees.
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    Stats of fisheries and disease control in the Republic of North Macedonia in the erly 21th centery
    (Balkan Scientific Association of Agricultural Economists, 2022-07-30)
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    This review aims to show the current state of fisheries in the Republic of Northern Macedonia as well as the strategy for the prevention and control of World Organization for Animal Health (OIE) notifiable diseases in aquaculture in order to comply with European legislation. At the moment, Macedonia produces about 1,700 tons, which dominates the production of trout with 63% and carp with about 29% of the total production. This sector has had a constant. Positive growth in recent years, attracting new investors, especially in cold-water aquaculture. Although the consummation of 5 kg per capita of fish is modest, domestic production does not satisfy their needs, so the market is supplemented with fish imports. One of the conditions for this sector to continue to grow at such a pace is to limit losses in aquaculture production. The most serious limitation that serious producers deal with is the appearance of infectious diseases.
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    Occurrence of Infectious hematopoietic necrosis, Koi herpesvirus disease, and Viral hemorrhagic septicemia in North Macedonia between 2015-2023
    (Macedonian Veterinary Review, 2025-03-15)
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    Zagorka Popova Hristovska
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    Viral hemorrhagic septicemia (VHS), Infectious hematopoietic necrosis (IHN), and Koi herpesvirus disease (KHVD) are listed diseases by the European Commission that pose significant threats to the global aquaculture industry, resulting in substantial economic losses and impacting fish health and welfare. Due to their rapid spread potential, it is crucial for member states to implement measures preventing their transmission to disease-free areas. In this study, we aimed to assess the presence or absence of these viruses in fish aquaculture facilities in North Macedonia. During 9 years of surveillance from 2015 to 2023, 1,527 samples were tested for VHS and IHN, and 2,760 samples were tested for KHVD from aquaculture sites across North Macedonia using molecular diagnostic techniques. Our results indicated the absence of VHS and KHVD in all tested samples. However, the number of IHN-affected farms increased from two in 2018 to 33 by 2023, persisting across multiple sites. Despite the absence of VHS and KHVD, the ongoing presence and increasing incidence of IHN highlight the need to assess the effectiveness of existing biosecurity measures and disease management practices in the region. Ongoing surveillance and stringent biosecurity measures are essential for controlling IHN and preventing the introduction of other viral pathogens. Strengthening these measures is vital to ensure the long-term sustainability of the aquaculture industry in North Macedonia.
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    Performances of oxygenation devices on trout farms in Republic of Serbia, Republic of North Macedonia, and Bosnia and Herzegovina
    (Centre for Evaluation in Education and Science (CEON/CEES), 2025)
    Čanak, Stevan
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    Radosavljević, Vladimir
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    Gligorević, Kosta
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    <jats:p>Dissolved oxygen is universally recognized as the most decisive limiting factor in intensive trout aquaculture, exerting profound effects not only on production efficiency but also on fish welfare, physiological stability, immune competence, and the capacity to resist disease. When oxygen levels fall below optimal thresholds, trout are exposed to chronic stress, impaired metabolic processes, reduced feed intake, and a heightened susceptibility to pathogens, all of which compromise both productivity and sustainability. To counter these risks, water oxygenation—defined as the technological process of enriching aquaculture water with pure oxygen—has become an indispensable intervention for modern trout farming. In Republic of Serbia and Republic of North Macedonia, oxygenation systems have been continuously applied for more than two decades, while in Bosnia and Herzegovina their use has been established for over ten years, collectively enabling production intensification, seasonal stability, and improved economic outcomes. This study explores the diversity of oxygenation technologies currently employed, ranging from diffuser systems and low‑head oxygenators to pressurized tanks and devices with rotating mechanical components. Particular emphasis is placed on their operational principles, efficiency parameters, and exploitation characteristics, with attention to how these technologies are adapted to the specific environmental conditions and management practices of rainbow trout farms in the region.</jats:p>
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    EPIDEMIOLOGY OF INFECTIOUS HEMATOPOIETIC NECROSIS, VIRAL HEMORRHAGIC SEPTICEMIA, AND KOI HERPES VIROSIS IN AQUACULTURED FISH IN THE REPUBLIC OF NORTH MACEDONIA
    (Ss. CYRIL AND METHODIUS UNIVERSITY IN SKOPJE, FACULTY OF VETERINARY MEDICINE – SKOPJE, 2025-07-14)
    This doctoral dissertation investigates the epidemiology of three significant viral diseases affecting cultured fish in the Republic of North Macedonia: Infectious Hematopoietic Necrosis (IHN), Viral Hemorrhagic Septicemia (VHS), and Koi Herpesvirus Disease (KHVD). The research was conducted from 2020 to 2024, analyzing samples from 57 trout farms and 36 carp farms. Advanced molecular techniques (RT-qPCR), genetic typing of isolates, histopathological analyses, and statistical models for assessing risk factors were employed. The analyses revealed that VHS and KHVD were not detected in any of the tested samples, confirming the country's disease-free status regarding these infections. In contrast, a concerning trend was noted regarding the spread of IHN among trout farms. The number of farms infected with IHN increased from two cases in 2018 to 36 in 2024. Genetic analysis revealed that all IHN isolates belong to the MAKIHNV1 genotype, exhibiting minimal genetic variation of less than 1.5%. This indicates a common infection source or limited viral evolution within the studied population. Histopathological examinations of IHN-positive fish revealed significant damage to the gill lamellae, kidney tissue degeneration, and inflammatory changes in the liver, confirming the virus's detrimental impact on fish health. Statistical analysis identified that the most significant risk factor for the spread of IHN was the purchase of live fish, particularly when farms relied on a single supplier. The frequency of purchases and the number of suppliers also significantly influenced the infection rate. Although biosecurity measures such as equipment and vehicle disinfection showed no significant impact on infection reduction, access control and quarantine implementation proved critical in preventing disease spread. These findings confirm the disease-free status for VHS and KHVD in the country but highlight an urgent need to strengthen biosecurity measures and monitoring to control IHN. The research emphasizes the importance of strategic management approaches in fish farms, including strict quarantine protocols upon fish introduction and enhanced biosecurity systems. This study provides vital epidemiological information on viral diseases in aquaculture and offers guidelines for reducing the risk of IHN in fish farming operations.
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    Genetic characterisation of Echinococcus granulosus sensu lato infecting ruminants in the Republic of North Macedonia
    (2025-12)
    Rashikj, LJubica
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    Zagorka Popova Hristovska
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    Betim Xhekaj