Mitevska, Elida
Preferred name
Mitevska, Elida
Official Name
Mitevska, Elida
Main Affiliation
Email
elida.mitevska@medf.ukim.edu.mk
10 results
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Item type:Publication, MORPHO-HISTOLOGICAL CHARACTERISTICS OF BRUISES WITH DIFFERENT AGE -qualitative study(Macedonian Association of Anatomists, 2022); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Evaluation of sperm quality in young men in North Macedonia(Институт за јавно здравје на Република Македонија = Institute of public health of Republic of Macedonia, 2023); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, MORPHOHISTOLOGICAL CHARACTERISTICS OF BRUISES WITH DIFFERENT AGE-QUANTITATIVE STUDY(Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 2022); ; ; Bruises as common skin lesions are important in forensic expert reports in determining the time of death in many cases of domestic violence or child abuse. The aim of this study was to present the differences between bruises with different time of origin by evaluation of their histological characteristics.The study included 60 human skin samples divided into control andexperimental groups A, B, C, D. Group A included skin samples with < 1day old bruises, group B included skin samples with 1-3 days old bruises, group C included skin samples with 3-7 days old bruises, and group D included skin samples with 7-14 days old bruises. Paraffin sections of the skin stained with Hematoxylin-eosin, Giemsa, Perl’s Prussian Blue and Masson-Goldner methods were evaluated by light microscopy and photodocumented,using stereological method for cell count and photodocumented.Histologicalanalysis in group A showed presence of dilated fibrous septa in dermis and hypodermis with extravasated erythrocytes and intense infiltration with neutrophils. In group B there was initial infiltration by macrophages. Group C also showed presence of macrophages, but itwas significantly more expressed than in group B (p<0.001). Group D showed a significant presence of macrophages when compared to groups B and C (p<0.001).Infiltration with neutrophils occurred shortly after the initiation of the bruise, while after few days of initiation, usually 1-3, there was macrophage infiltration. Presence of macrophages significantly increased as bruises age. These histological characteristics speak in favor of healing the bruises. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, METHODS USED IN DETERMINING THE AGE OF BRUISES(SHMSHM - AAMD, 2015); ; ; ; Bruises are common skin lesions that can be found in persons died a violent death and therefore have a forensic significance. In practice there are cases of violent death when it is difficult to determine with certainty the age of the bruises. But according to the biological processes associated with the reparation of the bruises, we could get reliable information about their age. Common methods for determining the age of the bruises, such as observation, colorimetry, spectrophotometry and patho-histological analysis are presented in this review paper. Determination of the age of bruises according to their color is a method used by most experts in the initial analysis. The observation of the yellow color, not brown or orange, points out a bruise that is not fresh. However, the possibility of visual observation is limited by the morphology and physiology of the human eye. Spectrophotometric analysis of data can offer useful and objective information. Histological analysis is suitable as a method only in post-mortem examinations. In this field of work there is a lack of published information on the histological analysis about the aging of bruises, which restricts the information for more precise determination of their age - Some of the metrics are blocked by yourconsent settings
Item type:Publication, HISTOLOGICAL STRUCTURE OF THE THYROID GLAND IN APOLIPOPROTEIN E DEFICIENT FEMALE MICE AFTER LEVOTHYROXINE APPLICATION(Macedonian Academy of Sciences and Arts, 2014); ; ; The aim of this study was to determine the histological characteristics of the thyroid gland in ApoE KO-/-mice following the application of levothyroxine (l-thyroxine). A total of 12 female ApoE KO-/- mice were divided into two groups. A control group of mice received distilled water and the experimental group received l-thyroxine dissolved in drinking water at a daily dose of 2 µg/ml, over 12 weeks. The paraffin sections were processed with the usual hae- matoxilin-eosin technique of staining. Qualitative histological analysis demonstrated: the presence of large distended follicles in the peripheral areas of the gland; fulfillment of the lumen of follicles with an ample amount of colloid; complete absence of resorptive vacuoles in the colloid; a flattened follicullar epithelium. Morpho- metric assessment showed a significant increase in the diameters of follicles in the peripheral areas of the gland and a significant decrease in the height of the follicullar epithelium (p < 0,001). Our results demonstrated that l-thyroxine causes characteristic morphological changes in the struc ture of the thyroid gland in the direction of the occurrence of hyperthyroidism. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Stereological study of the Wistar rats` thymus after application of high therapeutic doses of dexamethasone and medroxyprogesterone acetate(Macedonian Association of Anatomists and Morphologists, 2017); Aim: The aim of our study was to evaluate the eventual immunosuppressive effect of high therapeutic dose of medroxyprogesterone acetate (MPA), to determine morphological and stereological thymic tissue characteristics and to compare them with the morphological characteristics induced by the high therapeutic dexamethasone (Dex) dose. Material and Methods: A total of 36 female Wistar rats were divided into three groups. The control group of rats were administered physiological solution, whereas the experimental groups received Dex at a dose of 3 mg/kg bw and MPA at a dose of 150 mg/kg bw. Drugs were administered intramuscularly every day, in a period of 7 days. Thymus paraffin sections were stained according to the following methods: hematoxylin-eosin, periodic acid-Schiff and elastica-van Gieson. The stereological measurements were performed by using the Weibel’s multipurpose test system (M-42). Results: Histological analysis in the drug-treated rats showed disrupted thymic cytoarchitecture, lymphocyte density reduction which in some areas was to a degree of lymphocyte depletion, presence of Hassal’s corpuscles in thymic lobules medulla and abundant presence of interstitial connective tissue. Stereological analysis showed a significant reduction in the thymic parenchyma volume density, which was due to the more pronounced decrease in the thymic lobules cortex volume density and a significant increase of the interstitial connective tissue volume density. Conclusion: Our results showed that the high therapeutic dose of MPA provoked a marked reduction in immunocompetent lymphoid tissue, which led in changing the proportion of thymic structural components. These resulted in an immunosuppressive effect. Almost identical changes were caused by the high therapeutic dose of Dex, but thymic parenchyma was significantly more sensitive substrate for Dex than for MPA. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, CHANGES OF SPLEEN IN WISTAR RATS EXPOSED TO THERAPEUTIC DOSES OF DEXAMETHASONE AND MEDROXYPROGESTERONE ACETATE EVALUATED BY STEREOLOGICAL PARAMETERS(Macedonian Academy of Sciences and Arts, 2015); ; The aim of our investigation was to evaluate the immunosuppressive effect of medroxyprogesterone acetate (MPA) determining the volume densities of the structural components of the spleen. The volume densities of the same structural components of spleen were determined after administration of dexamethasone too, in order to see whether the morphological changes induced by MPA are in the same line with the changes caused by dexamethasone. 60 female Wistar rats were divided into 5 groups. The control group of rats was administered phy siological solution. The remaining, 4 experimental groups were administered: dexamethasone at a therapeutic daily dose of 0.6 mg/kg bw and maximal therapeutic dose of 3 mg/kg bw, and MPA at a therapeutic dose of 30 mg/kg bw and maximal therapeutic dose of 150 mg/kg bw. The drugs were applied intramuscularly for 7 days. Spleen paraffin sections were stained according to the methods: hematoxylin-eosin, Masson and Elastica van-Gieson. Stereological measurements were performed by using the Weibl’s multipurpose test system (M-42). The histological analyses of the structural components of the spleen in rats treated with dexametha sone and MPA have shown reduction of the white pulp and the marginal zone and an apparent decrease of the cellular density of the lymphocyte component of the pulp. The stereological analysis of the spleen showed significant decrease of the splenic pulp volume density and significant increase of the connective tissue volume density. Reducing the presence of splenic pulp was mainly due to the decrease in the volume density of all structural components of the white pulp. Changes were obser ved in all drug treated groups of rats. Our results have shown that the MPA provoked changes suggested atrophy of the spleen lymphoid tissue. Although the atrophic changes of the spleen were significant after the application of both dexamethasone and MPA, the white pulp was significantly more sensitive substrate for dexametha sone than for the MPA - Some of the metrics are blocked by yourconsent settings
Item type:Publication, The depth of the stromal invasion of cutaneous squamous cell carcinoma in correlation with tumor size and tumor differentiation(Macedonian Association of Pathology, 2016-09); ; ; ; Objective: The aim of this study was to determine the correlations between stromal invasion and the grade of differentiation as well as tumor’s size in squamous cell carcinoma of the skin (SCC). Material and Methods: Surgically resected skin specimens from 30 patients with cutaneous SCC, were included in the study. The hematoxylin-eosin stained histological sections containing the tumor tissue and the surrounding normal skin prepared from routinely processed paraffin blocks were analyzed by light microscopy. In each analyzed SCC, the degree of histological differentiation (G) and the postoperative tumor status (pT) of the neoplasm according to TNM classification (AJCC) were determined. The depth of stromal invasion in each case was measured on low power field (x40) using morphometry software. The distance from the basement membrane of the epidermis to the deepest invasive neoplastic focus of the tumor and the obtained values are presented in absolute numbers expressed in micrometers. Results: The SCC in 21 (70%) cases was classified as pT1 and in 9 (30%) cases as pT2 category tumor. Twelve tumors (40%) were classified as well (G1), 13 (43.3%) as moderately (G2), and 5 (16.7%) as poorly (G3) differentiated tumors. The depth of stromal invasion was ranging from 1561.2 μm to 13000.1 μm. A statistically significant difference was found between the depth of invasion in tumors belonging to different pT category (Mann-Whitney U test, p=0.003034 for pT1 and pT2), and different grade (Kruskal-Wallis test, p=0.00008 for G1, G2, G3). Conclusions: The depth of stromal invasion was higher in larger SCCs with a maximal diameter greater than 2 cm (pT2) and in poorly differentiated (G3) tumors. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Histological Characteristics of Bruises with Different Age(ID Design 2012/Scienfitic Foundation SPIROSKI, 2017-12-15); ; Biljana JaneskaBACKGROUND:In forensics bruises as injuries take an important part in the interpretation of the causes of death. Since activating the inflammatory response of the body in their formation, histological analysis of the bruised tissue can provide data on the determination the time when the injury occurred.AIM:The aim of this study is to compare the histological features of 1-day and 5-days old bruises.MATERIAL AND METHODS:Bruised human skin samples, 1-day old in group A and 5-day-old in group B, obtained at autopsy from individuals who died from a violent death, were analyzedin this study. The qualitativemicroscopic analysis was performedon serial paraffin sections of tissues stained with Hematoxylin-eosin and Pearls Prussian Blue method, using a light microscope connected to a digital camera.RESULTS:Qualitative histological analysis of the studied group A presented with fresh bruises, less than 24 hours old, showed ruptured smaller vessels and extravasated red blood cells intheconnectivetissue of the skin, with subsequent expansion and infiltration of fibrous septa of the skin. In the area of bleeding an initial infiltration by macrophages was observed. In the studied group B, presented with bruises 3-7 days old, histological analysis showed a marked presence of hemosiderin-ladenmacrophages and presence of hematoidin granules in the area of bleeding, as well as ruptured small blood vessels and red blood cells extravasation in the dilated fibrous septa. CONCLUSION: A detailed analysis of tissue changes in bruises every day from the initiation until their recovery, a detailed description of the histological finding can be given, which will be supported in the precise determination of the age of the injuries themselves - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Histophysiology of the spleen after application of medroxyprogesterone acetate(1998); ; ; ; The aim of our study was to determine the activity of medroxiprogesterone acetate (MPA) on the histophysiology of the biggest lymphatical organ, the spleen. The experiment was performed using 24 female Wister rats. The first, control group of 12 rats received physiological solution daily, whereas the other group, MPA in a dose of 15.0 mg/kg bw intramuscularly in a period of 30 days. The histological analysis of the spleen showed an obvious reduction of the lymph follicles which were in an involuntary phase with inactive germinal centers. Destructive changes in tissue were registered in the close distance of which a more intensive development of connective tissue was noticed into which collagen fibres predominate. The stereological analysis showed decrease of volume density of the white pulp from 13,42 ± 2,12% (medium value ± standard deviation) to 7,14 ± 1,92% (p < 0,001). The volume density of the marginal zone increased from 36,91 ± 4,44% to 52,38 ±15,14% (p< 0,001) and the volume density of the red pulp dropped from 49,69 ± 5,18% to 38,1 ± 7,15% (p< 0,001).