Kostovska, Nevena
Preferred name
Kostovska, Nevena
Official Name
Kostovska, Nevena
Main Affiliation
Email
nevena.kostovska@medf.ukim.edu.mk
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Item type:Publication, METHODS USED IN DETERMINING THE AGE OF BRUISES(SHMSHM - AAMD, 2015); ; ; ; Bruises are common skin lesions that can be found in persons died a violent death and therefore have a forensic significance. In practice there are cases of violent death when it is difficult to determine with certainty the age of the bruises. But according to the biological processes associated with the reparation of the bruises, we could get reliable information about their age. Common methods for determining the age of the bruises, such as observation, colorimetry, spectrophotometry and patho-histological analysis are presented in this review paper. Determination of the age of bruises according to their color is a method used by most experts in the initial analysis. The observation of the yellow color, not brown or orange, points out a bruise that is not fresh. However, the possibility of visual observation is limited by the morphology and physiology of the human eye. Spectrophotometric analysis of data can offer useful and objective information. Histological analysis is suitable as a method only in post-mortem examinations. In this field of work there is a lack of published information on the histological analysis about the aging of bruises, which restricts the information for more precise determination of their age - Some of the metrics are blocked by yourconsent settings
Item type:Publication, HISTOLOGICAL STRUCTURE OF THE THYROID GLAND IN APOLIPOPROTEIN E DEFICIENT FEMALE MICE AFTER LEVOTHYROXINE APPLICATION(Macedonian Academy of Sciences and Arts, 2014); ; ; The aim of this study was to determine the histological characteristics of the thyroid gland in ApoE KO-/-mice following the application of levothyroxine (l-thyroxine). A total of 12 female ApoE KO-/- mice were divided into two groups. A control group of mice received distilled water and the experimental group received l-thyroxine dissolved in drinking water at a daily dose of 2 µg/ml, over 12 weeks. The paraffin sections were processed with the usual hae- matoxilin-eosin technique of staining. Qualitative histological analysis demonstrated: the presence of large distended follicles in the peripheral areas of the gland; fulfillment of the lumen of follicles with an ample amount of colloid; complete absence of resorptive vacuoles in the colloid; a flattened follicullar epithelium. Morpho- metric assessment showed a significant increase in the diameters of follicles in the peripheral areas of the gland and a significant decrease in the height of the follicullar epithelium (p < 0,001). Our results demonstrated that l-thyroxine causes characteristic morphological changes in the struc ture of the thyroid gland in the direction of the occurrence of hyperthyroidism. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, CHANGES OF SPLEEN IN WISTAR RATS EXPOSED TO THERAPEUTIC DOSES OF DEXAMETHASONE AND MEDROXYPROGESTERONE ACETATE EVALUATED BY STEREOLOGICAL PARAMETERS(Macedonian Academy of Sciences and Arts, 2015); ; The aim of our investigation was to evaluate the immunosuppressive effect of medroxyprogesterone acetate (MPA) determining the volume densities of the structural components of the spleen. The volume densities of the same structural components of spleen were determined after administration of dexamethasone too, in order to see whether the morphological changes induced by MPA are in the same line with the changes caused by dexamethasone. 60 female Wistar rats were divided into 5 groups. The control group of rats was administered phy siological solution. The remaining, 4 experimental groups were administered: dexamethasone at a therapeutic daily dose of 0.6 mg/kg bw and maximal therapeutic dose of 3 mg/kg bw, and MPA at a therapeutic dose of 30 mg/kg bw and maximal therapeutic dose of 150 mg/kg bw. The drugs were applied intramuscularly for 7 days. Spleen paraffin sections were stained according to the methods: hematoxylin-eosin, Masson and Elastica van-Gieson. Stereological measurements were performed by using the Weibl’s multipurpose test system (M-42). The histological analyses of the structural components of the spleen in rats treated with dexametha sone and MPA have shown reduction of the white pulp and the marginal zone and an apparent decrease of the cellular density of the lymphocyte component of the pulp. The stereological analysis of the spleen showed significant decrease of the splenic pulp volume density and significant increase of the connective tissue volume density. Reducing the presence of splenic pulp was mainly due to the decrease in the volume density of all structural components of the white pulp. Changes were obser ved in all drug treated groups of rats. Our results have shown that the MPA provoked changes suggested atrophy of the spleen lymphoid tissue. Although the atrophic changes of the spleen were significant after the application of both dexamethasone and MPA, the white pulp was significantly more sensitive substrate for dexametha sone than for the MPA