Grdanoska, Tanja
Preferred name
Grdanoska, Tanja
Official Name
Grdanoska, Tanja
Main Affiliation
Email
tanja.grdanoska@medf.ukim.edu.mk
11 results
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Item type:Publication, Chlamydia pneumoniae and helicobacter pylori serology - importance in patients with coronary heart disease(Academy of Medical Sciences of Bosnia and Herzegovina, 2012); ; Chronic infections in CHD are due to one or both of the organisms Chlamydia pneumoniae and Helicobacter pylori. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Connection between Chlamydia trachomatis and carcinoma(Medical Facilty, Ss Cyril and Methodius University, Skopje, R Macedonia, 2019); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cranberry, a potential alternative treatment for urinary tract infections(Macedonian Association of Anatomists, 2023); ; ; ; Radomir JovchevskiVaccinium macrocarpon is perennial plant traditionally used as an herbal medicine in treatment and prevention of UTIs. Although the mechanisms of action are not jet fully understood it is presumed that they involve interferation with bacterial adhesion and changes in bacterial morphology mainly attributed to the plants proanthocyanidins. C ranberry extracts (CE) standardized for different concentrations of proanthocyanidinnes (PACs), CE in combination with antibiotics (norfloxacin and vancomy cin) and antibiotics alone (only antibiotics) were investigated for their effect on different strains of uropathogenic E.coli, S. saprophyticus and E. faecalis . As a source of CE we used commercial herbal supplements containing only Vaccinium macrocarpon extract (37.5 mg PACs) or CE in combination with D - manoza (25, 3 mg PACs). We used bacterial strains isolated from out patients with UTI s reffered for routine urine examination at the Institute of microbiology and parasitology. Sensitivity of the pathogen s to CE (as monoagent or combined in herbal mix) was evaluated with disc diffusion method. Our results showed stronger effect of CE on the growth of E.coli compared to G ram - positive strain s . S. saprophyticus strains were more susceptible to the extract/herbal mixes compared to the enterococci which predominantly presented as recalcitrant to the inhibitory activity of cranberry/herbal mixes. The sample size of this study was small to draw definite conclusions but our results illuminate avenues for future re search of the potential of cranberry as an alternative treatment in patients with UTIs. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Distribution of Clostridioides difficile ribotypes isolated from patients in North Macedonia - Update(Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 2023); ; As one of the major hospital pathogens Clostridioides difficile strains are constantly a subject of typing. PCR ribotyping is the standard molecular typing method for this bacteriumin Europe.The aim of this study wasto determine the distribution of C.difficileribotypes isolated from patients in North Macedonia.Eighty isolatesof C.difficile, isolated from the same number of patients`fecalsamples being sent to the Institute of Microbiology and Parasitology in Skopjein the periodfrom 2016to 2020in order to diagnose C.difficileinfection, were included.PCR ribotyping was performed by using primers and protocolsdescribed by Bidet. The final determination of the ribotypes was done by using the software BioNumerics.We determinedthe presence of 20 ribotypes. The most commonribotype was 001/072 represented with 32 (40%) isolates,followed by 014/020 represented with 10 (12,5%) isolates and ribotypes 002, 017 and 027 represented by 5 (6,2%) isolates each. All other ribotypes were represented by less than 4 isolates. Allribotype 001/072isolatesoriginated from patients of the “Mother Teresa”Clinical Center. Unlike in most of the European countries where the hypervirulent ribotype 027 is the dominant one, for a prolonged period of time001/072 has beenthe dominantC. difficileribotype isolated from patients in our country.Considering that most of the isolates of this ribotype hadoriginated from Surgicaland Internal DiseasesClinics in the “Mother Teresa” Clinical Center, we might assume that it is the endemic C. difficile strain there. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antimicrobial resistance in Gram-positive bacteria isolated from blood culture(Macedonian Association of Anatomists and Morphologists, 2024-10); ; ; ; Blood cultures are essential in diagnosing the existence of bacteria in the blood and they continue to be the gold standard for the diagnosis of septicemia. From Gram-positive bacteria, Staphylococcus aureus and Enterococcus spp. are the most common bacteria causing sepsis and septic shock. They are the leading causes of hospital-acquired infections globally. Aim of the paper was to identify Staphylococcus aureus and Enterococcus spp. from hemoculture and to determine their susceptibility to antibiotics. Blood samples from hospitalized patients admitted for routine laboratory testing at the Institute of Microbiology from January 2023 to December 2023 were included in the study. Blood cultures bottles were incubated in automated Bact/Alert system. Isolated colonies were identified using automated VITEK 2 Compact system. MSSA and MRSA were isolated in 48.2% and 51.8% respectively. All isolates 100% were resistant to penicillin. Intermediate sensitivity to levofloxacin 92%, 60% and ciprofloxacin 96%, 64% was observed in MSSA and MRSA strains. Resistance to gentamicin of 63% was seen in MSSA isolates. Non-VRE and VRE were isolated in 67% and 33% respectively. Non-VRE strains had higher resistance towards gentamicin 77%, streptomycin 66%. VRE strains were resistant to gentamicin 93%, ampicillin 85%. Our findings give analysis of Gram-positive bacteria isolated from hemoculture and their resistance pattern. The findings give clinicians an insight for the right treatment option. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Етиологија на инфекциите на долните дишни патишта во детската возраст и значењето на директниот микроскопски препарат во нивната дијагностика(Македонско лекарско друштво = Macedonian Medical Association, 2003); ; ; ; Spasenovski, TrajceЦел на испитувањето е да е направи анализа на етиологијата на инфекции на долните дишни патишта (ДДП) кај деца според две различни микробиолошки испитувања: културелно и серолошко; да се компарираат микробиолошките наоди според етиолошкиот агенс (бактериски, вирусни, мешани) со наодите во директиот микроскопски препарат. Беа испитани трахеални аспирати (ТА) и крв од вкупно 118 деца со инфекции на ДДП од Клиниката за детски болести (ноември 2001-март 2002). ТА се испитуваа културелно и микроскопски (епителни клетки и леукоцити). Наодите беа групирани во 3 микроскопски категории: I (>25 ЛЕ, висока гнојност, <10 еп. кл. незначителна контаминација); II (>25 еп. кл. значителна контаминација; <10 ЛЕ ниска гнојност) и III (<10 ЛЕ, ниска гнојност, , <10 еп. кл. незначителна контаминација). Во серумот со Pneumo-slide тест се одредуваа ИгМ антитела на 4 атипични бактерии и 5 вируси. Со позитивен микробиолошки наод беа 109 деца. Докажани беа 243 микроорганизми (58 култивабилни бактерии, 29 атипични бактерии и 156 вируси) со најчеста застапеност на: S. pneumonia (29), M pneumonia (21), H influenzaе (17), C. burneti (7), ентеробактерии (8), Инфлуенца вирус Б (82) и Инфлуенца вирус А (74). Дваесет (18,3%) деца имаа бактериска инфекција, 38 (34,8%) вирусна а 51 (46,7%) мешана инфекција (бактериско/вирусна). Според директните микроскопски препарати, 46 (38,9%) ТА беа во I микроскопска категорија, 31 (26,2%) во II и 41 (34,7%) во III. Микроскопското испитување покажа дека бројот на децата со вирусни инфекции е поголем, 64 (58,7%) од оние со мешани 25 (23,0%). Бактериолошката (културелна+директен микроскопски препарат) и серолошката дијагностика е значајна за детекција на вистински етиолошки агенси на инфекции на ДДП (бактериски, вирусни), за одредување квалитет на примероците за обработка, за примена на соодветна антимикробна терапија. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, ANTIMICROBIAL RESISTANCE IN GRAM NEGATIVE BACILLI ISOLATED FROM BLOOD CULTURE(University Ss. Cyril and Methodius in Skopje, 2023); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Seminal Fluid Microbiota and Male Infertility: An Emerging Frontier(Macedonian Association of Anatomists and Morphologists, 2024-10); ; ; ; Infertility is a multifaceted issue affecting millions globally, with male factors contributing to approximately 50% of cases. Traditionally, male infertility has been linked to genetic, hormonal, and environmental factors. However, emerging research highlights that disruptions or imbalances of the microbiota residing in the male reproductive tract, including the prostate, seminal vesicles, and urethra have significant implications for male reproductive health. Male urogenital system, particularly the urethra is home to a wide variety of microorganisms with rather high quantity. The composition of male genital microbiome can vary significantly between individuals and while the core microbiome is consised of a few dominant species a high variability is noted in minor species. It is predominantly composed of bacteria, with species from the genera Lactobacillus, Staphylococcus, Corynebacterium, and Propionibacterium being commonly found. Differentiating between pathogenic bacteria and the usual resident microflora is a difficulty in clinical practice, especially when it comes to male infertility. Furthermore, even though it is often benign, an imbalance in the commensal microbiota might encourage inflammation or foster an atmosphere that is favorable for pathogenic infections. Therefore, it is crucial to comprehend and maintain this equilibrium in order to create successful plans for the treatment and prevention of infertility. Traditional diagnostic approaches on male infertility focus on hormonal levels, sperm analysis, sperm culture and genetic factors, but unlike conventional culture methods which might find it difficult to distinguish between pathogenic and benign microorganisms because of genotypic and phenotypic overlaps and low pathogen presentation, developments in molecular methods for microbiota analysis, offer the possibility of more precise diagnosis and treatment of urogenital infections and imbalances. Modulating the genital microbiome could offer new treatment strategies for male infertility and related conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Attrition in Everyday Dental Clinical Practice(Macedonian Academy of Sciences and Arts/De Gruyter, 2024-12); ; Introduction Attrition, its prevention and therapy is a complex problem, with a multifactorial etiology. The aim of this paper is to examine the treatment of attrition in everyday dental clinical practice with the help of soft inserts. Material and method In this study, 30 patients were included, divided into two groups of 15 patients, the study and the control group. The examined patients had first degree of attrition diagnosed according to the Bardsley index, after which the condition was noted in four intervals: the initial situation, after 2, 4 and 6 months from the first visit. The research data were processed in Statistica for Windows 7.0 and SPSS version 20, and the same were presented graphically. Results In the study group with I degree of attrition, for p>0.05, no statistically significant difference was determined in the range of the Bardsley index between the four measurement times (Friedman Test: N=15; Chi-Square=7,200; df =3; p=0.0658). In the control group, for p<0.05, a significant difference was determined in the range of the Bardsley index between the four measured times (Friedman Test: N=15; Chi-Square=15.180; df=3; p=0.0017) with a significantly high value after 6 months. Discussion From the results obtained when comparing the study group in patients with I degree of attrition and the control group, a negative change from the parafunction bruxism was observed in 20% of the test group and 47% of the control group. The results of our research indicated that in patients with the first degree of attrition from the study group, soft dental inserts give significantly better results than untreated patients in the control group. This correlates with the research of Khayat N. et al. Conclusions From the results of the subjects in the group with I degree of attrition, we can conclude that the soft inserts has great efficiency as a means of management of this parafunction and therefore its application in daily therapy is recommended. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Distribution of Clostridium Difficile Ribotypes in Macedonian Patients and their Antimicrobial Susceptibility(Scientific Foundation Spiroski, 2019); ; BACKGROUND:Clostridium difficileis a major nosocomial pathogen. In Europe, this bacterium is mostly characterised by PCR ribotyping. Most of the Clostridium difficileinfections (CDI) are treated with vancomycin or metronidazole, although prolonged antibiotic use is considered as one ofthe main risk factors for CDI.AIM: This study aimedto detect the presence of various C. difficileribotypes in hospitalised patients and to investigate their toxigenicity and antibiotic susceptibility.MATERIAL AND METHODS:All stool samples obtained from each patient were inoculated on Columbia blood agar and cycloserine cefoxitine fructose agar (CCFA) for isolation of C. difficile. Glutamate dehydrogenase and toxins A and B were investigated by immunochromatographic tests. Final confirmation of the isolates was performed by Vitek 2 and MALDI-TOF. A total of 21 isolates were collected for further investigation. PCR ribotyping was performed as described by Janezic and Rupnik. PCR ribotype profiles were analysed using software (Bionumerics, Applied Maths).Antibiotic susceptibility was determined by E-tests for metronidazole, vancomycin, tetracycline, clindamycin, erythromycin, imipenem, ciprofloxacin and moxifloxacin.RESULTS:About 48% of C. difficileisolates belonged to ribotype 001/072. So, this ribotype was the most common ribotype in this study. The remaining 52% of C. difficileisolates consisted of 10 different ribotypes: 017, SLO 160, SLO 187, SLO 120, 255/258, 014/020, 046, 002, 070 and 027. Furthermore, 20 (95.2 %) out of 21 isolates of C. difficilewere toxigenic. Toxins A and B were detected simultaneously in 90.5 % of C. difficileisolates. Two isolates from the ribotype 017 were toxin B positive only. Treatments with any of the following antimicrobials: clindamycin, erythromycin, ciprofloxacin and moxifloxacin (as well as many other antibiotics), could be a risk factor for CDI due to the high resistance of the strains in this study. About 90% of the strains from the most common ribotype 001/072 have MICs for clindamycin and erythromycin >256 μg/ml. CONCLUSION: All strains isolated are highly resistant to ciprofloxacin. All strains were susceptible to vancomycin (median MIC was 0.63 μg/ml) and metronidazole (median MIC was 0.084 μg/ml), so these two antimicrobials remain optimal treatmentoption for CDI