Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/1628
Title: Assay of urinary excretion of polyphenols after ingestion of a cup of mountain tea (Sideritis scardica) measured by HPLC-DAD-ESI-MS/MS
Authors: Petreska Stanoeva, Jasmina
Stefova, Marina 
Keywords: Sideritis scardica, polyphenols, flavonoids, phenolic acids, metabolites, urinary excretion, hypolaetin, isoscutellarein
Issue Date: 6-Nov-2013
Publisher: American Chemical Society (ACS)
Journal: Journal of agricultural and food chemistry
Abstract: Flavonoids and phenolic acid metabolites excreted in human urine after ingestion of Sideritis scardica decoction with characterized polyphenolic composition were studied. A feeding study was carried out with 10 human volunteers, and urine samples were collected for 24 h after ingestion of the Sidertis decoction. Polyphenol metabolites were identified and quantified in urine samples by HPLC with tandem mass spectrometric detection. Thirty-one different metabolites of hypolaetin, methylhypolaetin, isoscutellarein, methylisoscutellarein, and apigenin and 32 phenolic acid metabolites were detected and quantified using a method validated for this purpose. The urinary excretion of polyphenol metabolites corresponded to 5% (n/n) of the intake of polyphenols from the Sideritis decoction. Flavonoid metabolites were dominant in urine samples with 87-94% of total polyphenolic metabolites content. The most abundant metabolites were methylhypolaetin and methylisoscutellarein glucuronides. Urinary excretion of isoscutellarein (35.61%) was 10 times higher than that of hypolaetin (3.67%). Apigenin also showed high urinary excretion (32.46%).
URI: http://hdl.handle.net/20.500.12188/1628
DOI: 10.1021/jf403052w
Appears in Collections:Faculty of Natural Sciences and Mathematics: Journal Articles

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