HPLC method validation and application for organic acid analysis in wine after solid-phase extraction

Journal
Macedonian Journal of Chemistry and Chemical Engineering
Date Issued
2016-12-27
Author(s)
Ivanova-Petropulos, Violeta
Tašev, Krste
DOI
10.20450/mjcce.2016.1073
Abstract
<jats:p><p>A solid-phase extraction method followed by reverse phase high-performance liquid chromatography (RP-HPLC) was optimized and validated for the quantitative determination of tartaric, malic, shikimic, lactic, citric and succinic acids in wine. Solid-phase extraction was carried out with C18 cartridges and extraction recoveries for all acids ranging from 98.3 to 103% were obtained. HPLC separation was performed with isocratic elution on a LiChrosorb RP-18 column (250 × 4.6 mm I.D., 5 µm) protected with the appropriate guard column. The mobile phase was a 5 mM solution of H<sub>3</sub>PO<sub>4</sub> with pH 2.1 at a flow rate of 1 ml/min. Detection of the organic acids was performed at 210 nm. The developed method was validated by checking its linearity, limit of detection (LOD), limit of quantification (LOQ), precision and recovery. The method was applied to the analysis of organic acids in Macedonian red and white wines.</p></jats:p>
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K. Tašev, M. Stefova, V. Ivanova-Petropulos, HPLC method validation and application for organic acid analysis in wine after solid-phase extraction, Macedonian Journal of Chemistry and Chemical Engineering, 35, (2), 225–233 (2016).
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