Please use this identifier to cite or link to this item:
http://hdl.handle.net/20.500.12188/15067
DC Field | Value | Language |
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dc.contributor.author | Gjorgieva, Darinka | en_US |
dc.contributor.author | Kadifkova Panovska, Tatjana | en_US |
dc.contributor.author | Ruskovska, Tatjana | en_US |
dc.contributor.author | Bačeva, Katerina | en_US |
dc.contributor.author | Stafilov, Trajče | en_US |
dc.date.accessioned | 2021-10-04T06:34:44Z | - |
dc.date.available | 2021-10-04T06:34:44Z | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0971-5894 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12188/15067 | - |
dc.description.abstract | The present study aimed to analyze the biological effects induced by bioaccumulation of metals in common bean (Phaseolus vulgaris L.). Effects of mineral nutrient imbalance, total antioxidants level and DNA damage induced by accumulation of heavy metals, were investigated in bean seedlings treated with two selected metal concentrations for 7 days. Metal content is analyzed by inductively coupled plasma - atomic emission spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay is used and Random Amplified Polymorphic DNA (RAPD) method was applied for investigation of DNA damages. The increasing metal concentration in the treatment medium changed synchronously metal content in samples, and decreased total antioxidant activity in all samples with exception only for samples treated with Ni and Cd. The obtained "DNA fingerprints" demonstrated that the increasing metal concentrations induced changes in RAPD profiles (disappearance and/or appearance of bands in comparison with untreated control samples). The highest number of missing bands was observed in samples treated with zinc (total 4 bands) and nickel (total 4 bands) at both concentrations. These results suggested that mineral nutrient imbalance is involved in changes of antioxidant levels and DNA damages of the seedlings, which may help to understand the mechanism of metal toxicity in plants. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.ispartof | Physiology and molecular biology of plants : an international journal of functional plant biology | en_US |
dc.title | Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals | en_US |
dc.identifier.doi | 10.1007/s12298-013-0196-0 | - |
dc.identifier.url | http://link.springer.com/content/pdf/10.1007/s12298-013-0196-0.pdf | - |
dc.identifier.url | http://link.springer.com/article/10.1007/s12298-013-0196-0/fulltext.html | - |
dc.identifier.url | http://link.springer.com/content/pdf/10.1007/s12298-013-0196-0 | - |
dc.identifier.volume | 19 | - |
dc.identifier.issue | 4 | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | Faculty of Natural Sciences and Mathematics: Journal Articles |
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