Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/14971
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dc.contributor.authorPetkovska, Anaen_US
dc.contributor.authorBabunovska, Hristinaen_US
dc.contributor.authorStefova, Marinaen_US
dc.date.accessioned2021-09-30T05:47:30Z-
dc.date.available2021-09-30T05:47:30Z-
dc.date.issued2011-12-05-
dc.identifier.urihttp://hdl.handle.net/20.500.12188/14971-
dc.description.abstract<jats:p>Quality control of pharmaceuticals requires development of fast, efficient and reliable methods for determination of active compounds as well as known and very often unknown impurities within defined concentration ranges. In this work, a simple and rapid HPLC-UV-DAD method for identification and quantification of pholcodine process related impurities and some degradation products was developed and validated. Pholcodine and its five structural analogues such as morphine, codeine, thebaine, oripavine, and papaverine were separated in less than 10 minutes using reversed phase LiChrospher C-8 column. For optimal chromatographic performance with reproducible retention times, gradient elution with 2% ammonium hydroxide in water and acetonitrile was used. The method was validated by establishing its selectivity, specifity, sensitivity, linearity, intra- and inter-day precision and robustness. All tested parameters confirmed that the method is suitable for determination of pholcodine and its five impurities in pharmaceutical drug samples. The results obtained from real sample analysis give support to the suitability of the proposed method for the purpose of quality control.</jats:p>en_US
dc.publisherSociety of Chemists and Technologists of Macedoniaen_US
dc.relation.ispartofMacedonian Journal of Chemistry and Chemical Engineeringen_US
dc.titleFast and selective HPLC-DAD method for determination of pholcodine and related substancesen_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
crisitem.author.deptFaculty of Natural Sciences and Mathematics-
Appears in Collections:Faculty of Natural Sciences and Mathematics: Journal Articles
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XIII_1764.pdfA. Petkovska, H. Babunovska, M. Stefova, Fast and selective HPLC-DAD method for determination of pholcodine and related substances, Macedonian Journal of Chemistry and Chemical Engineering, 30(2), 139–149 (2011).1.44 MBAdobe PDFView/Open
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